The cryogenic temperatures at which dynamic nuclear polarization (DNP) solid-state NMR experiments need to be carried out cause line-broadening, an effect that is especially detrimental for crowded protein spectra. By increasing the magnetic field strength from 600 to 800 MHz, the resolution of DNP spectra of type III secretion needles (T3SS) could be improved by 22 %, indicating that inhomogeneous broadening is not the dominant effect that limits the resolution of T3SS needles under DNP conditions. The outstanding spectral resolution of this system under DNP conditions can be attributed to its low overall flexibility.
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High resolution observed in 800Â*MHz DNP spectra of extremely rigid type III secretion needles
High resolution observed in 800Â*MHz DNP spectra of extremely rigid type III secretion needles
Abstract
The cryogenic temperatures at which dynamic nuclear polarization (DNP) solid-state NMR experiments need to be carried out cause line-broadening, an effect that is especially detrimental for crowded protein spectra. By increasing the magnetic field strength from 600 to 800Â*MHz, the resolution of DNP spectra of type III secretion needles (T3SS) could be improved by 22Â*%, indicating that inhomogeneous broadening is not the dominant effect that limits...
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06-30-2016 02:26 AM
[NMR paper] NMR Characterization of the Type III Secretion System Tip Chaperone Protein PcrG of Pseudomonas aeruginosa.
NMR Characterization of the Type III Secretion System Tip Chaperone Protein PcrG of Pseudomonas aeruginosa.
Related Articles NMR Characterization of the Type III Secretion System Tip Chaperone Protein PcrG of Pseudomonas aeruginosa.
Biochemistry. 2015 Oct 9;
Authors: Chaudhury S, Nordhues BA, Kaur K, Zhang N, De Guzman RN
Abstract
Lung infection with Pseudomonas aeruginosa is the leading cause of death among cystic fibrosis patients. To initiate infection, P. aeruginosa assembles a protein nanomachine, the type III secretion...
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10-10-2015 06:47 PM
[NMR paper] High-resolution structure of the Shigella type-III secretion needle by solid-state NMR and cryo-electron microscopy.
High-resolution structure of the Shigella type-III secretion needle by solid-state NMR and cryo-electron microscopy.
High-resolution structure of the Shigella type-III secretion needle by solid-state NMR and cryo-electron microscopy.
Nat Commun. 2014;5:4976
Authors: Demers JP, Habenstein B, Loquet A, Kumar Vasa S, Giller K, Becker S, Baker D, Lange A, Sgourakis NG
Abstract
We introduce a general hybrid approach for determining the structures of supramolecular assemblies. Cryo-electron microscopy (cryo-EM) data define the overall...
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09-30-2014 02:18 PM
[NMR paper] NMR Model of PrgI-SipD Interaction and its Implications in the Needle-Tip Assembly of the Salmonella Type III Secretion System.
NMR Model of PrgI-SipD Interaction and its Implications in the Needle-Tip Assembly of the Salmonella Type III Secretion System.
Related Articles NMR Model of PrgI-SipD Interaction and its Implications in the Needle-Tip Assembly of the Salmonella Type III Secretion System.
J Mol Biol. 2014 Jun 18;
Authors: Rathinavelan T, Lara-Tejero M, Lefebre M, Chatterjee S, McShan AC, Guo DC, Tang C, Galan JE, De Guzman RN
Abstract
Salmonella and other pathogenic bacteria use the type III secretion system (T3SS) to inject virulence proteins...
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06-22-2014 12:24 PM
[NMR paper] Biomass production of site selective 13C/15N nucleotides using wild type and a transketolase E. coli mutant for labeling RNA for high resolution NMR.
From Mendeley Biomolecular NMR group:
Biomass production of site selective 13C/15N nucleotides using wild type and a transketolase E. coli mutant for labeling RNA for high resolution NMR.
Journal of biomolecular NMR (2012). Volume: 52, Issue: 2. Pages: 103-14. Chandar S Thakur, Yiling Luo, Bin Chen, Nadukkudy V Eldho, T Kwaku Dayie et al.
Characterization of the structure and dynamics of nucleic acids by NMR benefits significantly from position specifically labeled nucleotides. Here an E. coli strain deficient in the transketolase gene (tktA) and grown on glucose that is labeled at...
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10-17-2013 12:49 PM
[NMR paper] Biomass production of site selective 13C/15N nucleotides using wild type and a transketolase E. coli mutant for labeling RNA for high resolution NMR.
From Mendeley Biomolecular NMR group:
Biomass production of site selective 13C/15N nucleotides using wild type and a transketolase E. coli mutant for labeling RNA for high resolution NMR.
Journal of biomolecular NMR (2012). Volume: 52, Issue: 2. Pages: 103-14. Chandar S Thakur, Yiling Luo, Bin Chen, Nadukkudy V Eldho, T Kwaku Dayie et al.
Characterization of the structure and dynamics of nucleic acids by NMR benefits significantly from position specifically labeled nucleotides. Here an E. coli strain deficient in the transketolase gene (tktA) and grown on glucose that is labeled at...
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08-24-2012 08:01 PM
TSAR: a program for automatic resonance assignment using 2D cross-sections of high dimensionality, high-resolution spectra
TSAR: a program for automatic resonance assignment using 2D cross-sections of high dimensionality, high-resolution spectra
Abstract While NMR studies of proteins typically aim at structure, dynamics or interactions, resonance assignments represent in almost all cases the initial step of the analysis. With increasing complexity of the NMR spectra, for example due to decreasing extent of ordered structure, this task often becomes both difficult and time-consuming, and the recording of high-dimensional data with high-resolution may be essential. Random sampling of the evolution time space,...
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07-20-2012 11:13 PM
The NMR Structure of FliK, the Trigger for the Switch of Substrate Specificity in the Flagellar Type III Secretion Apparatus.
The NMR Structure of FliK, the Trigger for the Switch of Substrate Specificity in the Flagellar Type III Secretion Apparatus.
The NMR Structure of FliK, the Trigger for the Switch of Substrate Specificity in the Flagellar Type III Secretion Apparatus.
J Mol Biol. 2011 Apr 12;
Authors: Mizuno S, Amida H, Kobayashi N, Aizawa SI, Tate SI
The flagellar cytoplasmic protein FliK controls hook elongation by two successive events: by determining hook length and by stopping the supply of hook protein. These two distinct roles are assigned to different...
High resolution observed in 800 MHz DNP spectra of extremely rigid type III secretion needles #DNPNMR
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