[1-(13)C]pyruvate is the most widely used hyperpolarized metabolic magnetic resonance imaging agent. Using a custom-built 7.0 T polarizer operating at 1.0 K and trityl radical-doped [1-(13)C]pyruvic acid, unextrapolated solution-state (13)C polarization greater than 60% was measured after dissolution and rapid transfer to a spectrometer magnet, demonstrating the signal enhancement attainable using optimized hardware. Slower rates of polarization under these conditions can be largely overcome with higher radical concentrations.
[NMR paper] Biosilica-Entrapped Enzymes Studied by Using Dynamic Nuclear-Polarization-Enhanced High-Field NMR Spectroscopy.
Biosilica-Entrapped Enzymes Studied by Using Dynamic Nuclear-Polarization-Enhanced High-Field NMR Spectroscopy.
Biosilica-Entrapped Enzymes Studied by Using Dynamic Nuclear-Polarization-Enhanced High-Field NMR Spectroscopy.
Chemphyschem. 2015 Aug 12;
Authors: Ravera E, Michaelis VK, Ong TC, Keeler EG, Martelli T, Fragai M, Griffin RG, Luchinat C
Abstract
Enzymes are used as environmentally friendly catalysts in many industrial applications, and are frequently immobilized in a matrix to improve their chemical stability for...
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08-13-2015 02:00 PM
[NMR paper] NMR-based structural biology enhanced by dynamic nuclear polarization at high magnetic field.
NMR-based structural biology enhanced by dynamic nuclear polarization at high magnetic field.
http://www.bionmr.com//www.ncbi.nlm.nih.gov/corehtml/query/egifs/http:--production.springer.de-OnlineResources-Logos-springerlink.gif Related Articles NMR-based structural biology enhanced by dynamic nuclear polarization at high magnetic field.
J Biomol NMR. 2014 Nov;60(2-3):157-68
Authors: Koers EJ, van der Cruijsen EA, Rosay M, Weingarth M, Prokofyev A, Sauvée C, Ouari O, van der Zwan J, Pongs O, Tordo P, Maas WE, Baldus M
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06-24-2015 01:08 PM
The role of the glassy dynamics and thermal mixing in the dynamic nuclear polarization and relaxation mechanisms of pyruvic acid
From The DNP-NMR Blog:
The role of the glassy dynamics and thermal mixing in the dynamic nuclear polarization and relaxation mechanisms of pyruvic acid
Filibian, M., et al., The role of the glassy dynamics and thermal mixing in the dynamic nuclear polarization and relaxation mechanisms of pyruvic acid. Phys Chem Chem Phys, 2014. 16(48): p. 27025-36.
http://www.ncbi.nlm.nih.gov/pubmed/25382595
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03-27-2015 11:59 PM
NMR-based structural biology enhanced by dynamic nuclear polarization at high magnetic field
From The DNP-NMR Blog:
NMR-based structural biology enhanced by dynamic nuclear polarization at high magnetic field
Koers, E.J., et al., NMR-based structural biology enhanced by dynamic nuclear polarization at high magnetic field. J Biomol NMR, 2014. 60(2-3): p. 157-68.
http://www.ncbi.nlm.nih.gov/pubmed/25284462
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11-19-2014 04:32 PM
NMR-based structural biology enhanced by dynamic nuclear polarization at high magnetic field
NMR-based structural biology enhanced by dynamic nuclear polarization at high magnetic field
Abstract
Dynamic nuclear polarization (DNP) has become a powerful method to enhance spectroscopic sensitivity in the context of magnetic resonance imaging and nuclear magnetic resonance spectroscopy. We show that, compared to DNP at lower field (400Â*MHz/263Â*GHz), high field DNP (800Â*MHz/527Â*GHz) can significantly enhance spectral resolution and allows exploitation of the paramagnetic relaxation properties of DNP polarizing agents as direct structural...
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10-05-2014 09:01 AM
High field dynamic nuclear polarization NMR with surfactant sheltered biradicals
From The DNP-NMR Blog:
High field dynamic nuclear polarization NMR with surfactant sheltered biradicals
Kiesewetter, M.K., et al., High field dynamic nuclear polarization NMR with surfactant sheltered biradicals. J Phys Chem B, 2014. 118(7): p. 1825-30.
http://www.ncbi.nlm.nih.gov/pubmed/24506193
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05-14-2014 05:14 PM
Topical Developments in High-Field Dynamic Nuclear Polarization
From The DNP-NMR Blog:
Topical Developments in High-Field Dynamic Nuclear Polarization
Michaelis, V.K., et al., Topical Developments in High-Field Dynamic Nuclear Polarization. Israel Journal of Chemistry, 2014. 54(1-2): p. 207-221.
http://dx.doi.org/10.1002/ijch.201300126
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04-05-2014 01:35 AM
High-Field (13)C Dynamic Nuclear Polarization with a Radical Mixture
From the The DNP-NMR Blog:
High-Field (13)C Dynamic Nuclear Polarization with a Radical Mixture
Michaelis, V.K., et al., High-Field (13)C Dynamic Nuclear Polarization with a Radical Mixture. J Am Chem Soc, 2013.
http://www.ncbi.nlm.nih.gov/pubmed/23373472
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High-field dissolution dynamic nuclear polarization of [1-(13)C]pyruvic acid
Linear Mode
