BioNMR
NMR aggregator & online community since 2003
BioNMR    
Learn or help to learn NMR - get free NMR books!
 

Go Back   BioNMR > NMR community > News from NMR blogs
Advanced Search
Home Forums Wiki NMR feeds Downloads Register Today's Posts



Jobs Groups Conferences Literature Pulse sequences Software forums Programs Sample preps Web resources BioNMR issues


Webservers
NMR processing:
MDD
NMR assignment:
Backbone:
Autoassign
MARS
UNIO Match
PINE
Side-chains:
UNIO ATNOS-Ascan
NOEs:
UNIO ATNOS-Candid
UNIO Candid
ASDP
Structure from NMR restraints:
Ab initio:
GeNMR
Cyana
XPLOR-NIH
ASDP
UNIO ATNOS-Candid
UNIO Candid
Fragment-based:
BMRB CS-Rosetta
Rosetta-NMR (Robetta)
Template-based:
GeNMR
I-TASSER
Refinement:
Amber
Structure from chemical shifts:
Fragment-based:
WeNMR CS-Rosetta
BMRB CS-Rosetta
Homology-based:
CS23D
Simshift
Torsion angles from chemical shifts:
Preditor
TALOS
Promega- Proline
Secondary structure from chemical shifts:
CSI (via RCI server)
TALOS
MICS caps, β-turns
d2D
PECAN
Flexibility from chemical shifts:
RCI
Interactions from chemical shifts:
HADDOCK
Chemical shifts re-referencing:
Shiftcor
UNIO Shiftinspector
LACS
CheckShift
RefDB
NMR model quality:
NOEs, other restraints:
PROSESS
PSVS
RPF scores
iCing
Chemical shifts:
PROSESS
CheShift2
Vasco
iCing
RDCs:
DC
Anisofit
Pseudocontact shifts:
Anisofit
Protein geomtery:
Resolution-by-Proxy
PROSESS
What-If
iCing
PSVS
MolProbity
SAVES2 or SAVES4
Vadar
Prosa
ProQ
MetaMQAPII
PSQS
Eval123D
STAN
Ramachandran Plot
Rampage
ERRAT
Verify_3D
Harmony
Quality Control Check
NMR spectrum prediction:
FANDAS
MestReS
V-NMR
Flexibility from structure:
Backbone S2
Methyl S2
B-factor
Molecular dynamics:
Gromacs
Amber
Antechamber
Chemical shifts prediction:
From structure:
Shiftx2
Sparta+
Camshift
CH3shift- Methyl
ArShift- Aromatic
ShiftS
Proshift
PPM
CheShift-2- Cα
From sequence:
Shifty
Camcoil
Poulsen_rc_CS
Disordered proteins:
MAXOCC
Format conversion & validation:
CCPN
From NMR-STAR 3.1
Validate NMR-STAR 3.1
NMR sample preparation:
Protein disorder:
DisMeta
Protein solubility:
camLILA
ccSOL
Camfold
camGroEL
Zyggregator
Isotope labeling:
UPLABEL
Solid-state NMR:
sedNMR


Reply
 
Thread Tools Search this Thread Rate Thread Display Modes
  #1  
Old 08-21-2010, 08:15 PM
nmrlearner's Avatar
Senior Member
 
Join Date: Jan 2005
Posts: 23,777
Points: 193,617, Level: 100
Points: 193,617, Level: 100 Points: 193,617, Level: 100 Points: 193,617, Level: 100
Level up: 0%, 0 Points needed
Level up: 0% Level up: 0% Level up: 0%
Activity: 50.7%
Activity: 50.7% Activity: 50.7% Activity: 50.7%
Last Achievements
Award-Showcase
NMR Credits: 0
NMR Points: 193,617
Downloads: 0
Uploads: 0
Default Hahn Echo for 11B Background Suppression in Solids

Hahn Echo for 11B Background Suppression in Solids

Solids NMR probes often contain boron rich parts near the coil in which the sample resides. Boron nitride, in particular, is a material very commonly used. This can be very problematic if one wishes to collect 11B NMR data, in that a strong background signal may be observed. Even though these parts are not directly inside the coil with the sample, they do experience a small amount of rf from the coil and the coil does detect a 11B NMR signal from them. One simple way to avoid this problem is to use a Hahn echo to observe the 11B spectrum. Unlike the sample inside the coil, which experiences the 90° and 180° pulses required for the Hahn echo, the boron rich parts outside of the coil experience pulses of much smaller flip angles and therefore the echo signal from them is much reduced. This is illustrated in the figure below, which shows 11B NMR spectra collected in a 4 mm MAS probe without magic angle spinning. In the lower trace a simple one-pulse measurement was made with high power 1H decoupling. The spectrum contains a very large background signal and it is very difficult to extract any useful information from the data. The spectrum in the upper trace was collected with a Hahn echo with high power 1H decoupling during the acquisition. The background signal is completely removed revealing a beautiful, information-rich line shape for the central transition resulting from the second order quadrupolar and chemical shielding anisotropy interactions.

Thank you to Joseph Weiss of David Bryce's laboratory for providing the sample and collecting the data. See more of Joseph's beautiful 11B spectra here:

Joseph W.E. Weiss and David L. Bryce J. Phys. Chem. 114 (2010) ASAP.
http://dx.doi.org/10.1021/jp101416k




Source: University of Ottawa NMR Facility Blog
Reply With Quote


Did you find this post helpful? Yes | No

Reply
Similar Threads
Thread Thread Starter Forum Replies Last Post
[KPWU blog] [PyMOL] gradient background color
gradient background color Starting at *PyMOL 1.4, *single color for the background is no longer the only choice. By using 3 PyMOL commands, the gradient background color can be easily shown. The only thing users have to know is that command “ray” doesn’t support this gradient color yet. So if you type “ray” in the pymol terminal , http://stats.wordpress.com/b.gif?host=kpwu.wordpress.com&blog=76132&post=619&subd=kpwu&ref=&feed=1 Go to KPWU blog to read complete post.
nmrlearner News from NMR blogs 0 11-15-2011 11:36 PM
[U. of Ottawa NMR Facility Blog] Background Suppression in Liquids
Background Suppression in Liquids High resolution NMR probes for liquids may contain parts near the coil consiting of the nuclei being observed. The parts give rise to background signals which can severely affect the NMR data. When observing11B, there is a background signal from boron containing parts near the coil and also the borosilicate glass in the NMR tube containing the sample. Cory and Ritchey* introduced a very simple, clever method to suppress background signals in 1988. Their method uses a composite pulse, consisting of a 90° and two 180° pulses with appropriate phase...
nmrlearner News from NMR blogs 0 08-21-2010 08:15 PM
[Stan NMR blog] Field noise effects on NMR signals: Hahn echoes and CPMG
Field noise effects on NMR signals: Hahn echoes and CPMG An article about the effects of field noise on spin echoes and CPMG decays. More...
nmrlearner News from NMR blogs 0 08-21-2010 06:14 PM
[NMRpipe Yahoo group] Re: problem with fast 3D in echo anti echo mode on Bruker
Re: problem with fast 3D in echo anti echo mode on Bruker The NMRPipe gradient-shuffling scheme will often result in a spectrum which requires a 90-degree phase correction in the gradient enhanced dimension. In this More...
nmrlearner News from other NMR forums 0 08-18-2010 11:15 AM
[NMRpipe Yahoo group] Re: problem with fast 3D in echo anti echo mode on Bruker
Re: problem with fast 3D in echo anti echo mode on Bruker Helene Check your second 13C-1H plane; bet all the signal is there. Paul -- Paul R. Gooley, PhD Department of Biochemistry and Molecular Biology and Bio21 More...
nmrlearner News from other NMR forums 0 08-18-2010 11:15 AM
[NMRpipe Yahoo group] problem with fast 3D in echo anti echo mode on Bruker
problem with fast 3D in echo anti echo mode on Bruker Hi I am trying to process a HNCA 3D experiments acquired with the standard bruker pulse schemes of fast experiments (Brutscher et al) which are recorded with More...
nmrlearner News from other NMR forums 0 08-18-2010 11:15 AM



Posting Rules
You may not post new threads
You may not post replies
You may not post attachments
You may not edit your posts

BB code is On
Smilies are On
[IMG] code is On
HTML code is Off
Trackbacks are Off
Pingbacks are Off
Refbacks are Off



BioNMR advertisements to pay for website hosting and domain registration. Nobody does it for us.



Powered by vBulletin® Version 3.7.3
Copyright ©2000 - 2024, Jelsoft Enterprises Ltd.
Copyright, BioNMR.com, 2003-2013
Search Engine Friendly URLs by vBSEO 3.6.0

All times are GMT. The time now is 10:58 AM.


Map