PURPOSE: There is great potential for real-time investigation of metabolism with MRS and hyperpolarized (HP) (13) C agents. Unfortunately, HP technology has high associated costs and efficiency limitations that may constrain in vivo studies involving many animals. To improve the throughput of preclinical investigations, we evaluate the feasibility of performing HP MRS on multiple animals simultaneously. METHODS: Simulations helped assess the viability of a dual-coil strategy for spatially localized multivolume MRS. A dual-mouse system was assembled and characterized with bench- and scanner-based experiments. Enzyme phantoms mixed with HP [1-(13) C] pyruvate emulated real-time metabolism and offered a controlled mechanism for evaluating system performance. Finally, a normal mouse and a mouse bearing a subcutaneous xenograft of colon cancer were simultaneously scanned in vivo using an agent containing HP [1-(13) C] pyruvate. RESULTS: Geometric separation/rotation, active decoupling, and use of low input impedance preamplifiers permitted an encode-by-channel approach for spatially localized MRS. A precalibrated shim allowed straightforward metabolite differentiation in enzyme phantom and in vivo experiments at 7 Tesla, with performance similar to conventional acquisitions. CONCLUSION: The initial feasibility of multi-animal HP (13) C MRS was established. Throughput scales with the number of simultaneously scanned animals, demonstrating the potential for significant improvements in study efficiency.
Systematic T1 improvement for hyperpolarized (129)xenon
From The DNP-NMR Blog:
Systematic T1 improvement for hyperpolarized (129)xenon
Repetto M, Babcock E, Blumler P, Heil W, Karpuk S, Tullney K. Systematic T1 improvement for hyperpolarized (129)xenon. J Magn Reson. 2015;252(0):163-9.
http://www.ncbi.nlm.nih.gov/pubmed/25702572
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05-19-2015 09:10 AM
Simultaneous hyperpolarized (13)C-pyruvate MRI and (18)F-FDG-PET in cancer (hyperPET): feasibility of a new imaging concept using a clinical PET/MRI scanner
From The DNP-NMR Blog:
Simultaneous hyperpolarized (13)C-pyruvate MRI and (18)F-FDG-PET in cancer (hyperPET): feasibility of a new imaging concept using a clinical PET/MRI scanner
Gutte, H., et al., Simultaneous hyperpolarized (13)C-pyruvate MRI and (18)F-FDG-PET in cancer (hyperPET): feasibility of a new imaging concept using a clinical PET/MRI scanner. American Journal of Nuclear Medicine and Molecular Imaging, 2015. 5(1): p. 38-45.
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4299777/
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05-14-2015 04:52 AM
The Feasibility of Formation and Kinetics of NMR Signal Amplification by Reversible Exchange (SABRE) at High Magnetic Field (9.4 T)
From The DNP-NMR Blog:
The Feasibility of Formation and Kinetics of NMR Signal Amplification by Reversible Exchange (SABRE) at High Magnetic Field (9.4 T)
Barskiy, D.A., et al., The feasibility of formation and kinetics of NMR signal amplification by reversible exchange (SABRE) at high magnetic field (9.4 T). J Am Chem Soc, 2014. 136(9): p. 3322-5.
http://www.ncbi.nlm.nih.gov/pubmed/24528143
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05-19-2014 09:25 PM
Hyperpolarized H2O MR angiography
From The DNP-NMR Blog:
Hyperpolarized H2O MR angiography
Ardenkjaer-Larsen, J.H., et al., Hyperpolarized H2 O MR angiography. Magn Reson Med, 2014. 71(1): p. 50-6.
http://www.ncbi.nlm.nih.gov/pubmed/24243653
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03-28-2014 04:59 PM
Cardiovascular Applications of Hyperpolarized MRI
From The DNP-NMR Blog:
Cardiovascular Applications of Hyperpolarized MRI
Tyler, D., Cardiovascular Applications of Hyperpolarized MRI. Curr Cardiovasc Imaging Rep, 2011. 4(2): p. 108-115.
http://dx.doi.org/10.1007/s12410-011-9066-8
Many applications of MRI are limited by an inherently low sensitivity. Previous attempts to overcome this insensitivity have focused on the use of MRI systems with stronger magnetic fields. However, the gains that can be achieved in this way are relatively small and increasing the magnetic field invariably leads to greater technical...
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07-15-2013 07:17 PM
[NMR paper] EC-SPE-stripline-NMR analysis of reactive products: a feasibility study.
EC-SPE-stripline-NMR analysis of reactive products: a feasibility study.
Related Articles EC-SPE-stripline-NMR analysis of reactive products: a feasibility study.
Anal Bioanal Chem. 2013 Jun 30;
Authors: Falck D, Oosthoek-de Vries AJ, Kolkman A, Lingeman H, Honing M, Wijmenga SS, Kentgens AP, Niessen WM
Abstract
Flow-through electrochemical conversion (EC) of drug-like molecules was hyphenated to miniaturized nuclear magnetic resonance spectroscopy (NMR) via on-line solid-phase extraction (SPE). After EC of the prominent p38?...
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07-03-2013 01:46 PM
Perspectives of hyperpolarized noble gas MRI beyond 3He
From the The DNP-NMR Blog:
Perspectives of hyperpolarized noble gas MRI beyond 3He
Lilburn, D.M.L., G.E. Pavlovskaya, and T. Meersmann, Perspectives of hyperpolarized noble gas MRI beyond 3He. J. Magn. Reson., 2013. 229(0): p. 173-186.
http://www.sciencedirect.com/science/article/pii/S1090780712003588
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04-15-2013 08:52 AM
The feasibility of parameterizing four-state equilibria using relaxation dispersion measurements
The feasibility of parameterizing four-state equilibria using relaxation dispersion measurements
Abstract Coupled equilibria play important roles in controlling information flow in biochemical systems, including allosteric molecules and multidomain proteins. In the simplest case, two equilibria are coupled to produce four interconverting states. In this study, we assessed the feasibility of determining the degree of coupling between two equilibria in a four-state system via relaxation dispersion measurements. A major bottleneck in this effort is the lack of efficient approaches to...
Feasibility of multianimal hyperpolarized (13) C MRS
Linear Mode
