Dynamic nuclear polarization (DNP) can enhance NMR sensitivity by orders of magnitude by transferring spin polarization from electron paramagnetic resonance (EPR) to NMR. However, paramagnetic DNP polarizing agents can have deleterious effects on NMR signals. Electron spin decoupling can mitigate these paramagnetic relaxation effects. We demonstrate electron decoupling experiments in conjunction with DNP and magic-angle-spinning NMR spectroscopy. Following a DNP and spin diffusion period, the microwave irradiation frequency is quickly tuned on-resonance with electrons on the DNP polarizing agent. The electron decoupling performance shows a strong dependence on the microwave frequency and DNP polarization time. Microwave frequency sweeps through the EPR line shape are shown as a time domain strategy to significantly improve electron decoupling. For 13C spins on biomolecules frozen in a glassy matrix, electron decoupling reduces the line widths by 11% (47 Hz) and increases the intensity by 14%.
p.p1 {margin: 0.0px 0.0px 0.0px 0.0px; font: 12.0px Helvetica} p.p1 {margin: 0.0px 0.0px 0.0px 0.0px; font: 12.0px Helvetica}
Effect of electron spectral diffusion on static dynamic nuclear polarization at 7 Tesla #DNPNMR
From The DNP-NMR Blog:
Effect of electron spectral diffusion on static dynamic nuclear polarization at 7 Tesla #DNPNMR
p.p1 {margin: 0.0px 0.0px 0.0px 36.0px; text-indent: -36.0px; font: 12.0px Helvetica}
Leavesley, A., et al., Effect of electron spectral diffusion on static dynamic nuclear polarization at 7 Tesla. Phys. Chem. Chem. Phys., 2017. 19(5): p. 3596-3605.
https://www.ncbi.nlm.nih.gov/pubmed/28094364
nmrlearner
News from NMR blogs
0
06-02-2017 08:33 PM
Temperature dependence of cross-effect dynamic nuclear polarization in rotating solids: advantages of elevated temperatures #DNPNMR
From The DNP-NMR Blog:
Temperature dependence of cross-effect dynamic nuclear polarization in rotating solids: advantages of elevated temperatures #DNPNMR
p.p1 {margin: 0.0px 0.0px 0.0px 36.0px; text-indent: -36.0px; font: 12.0px Helvetica}
Geiger, M.A., et al., Temperature dependence of cross-effect dynamic nuclear polarization in rotating solids: advantages of elevated temperatures. Phys. Chem. Chem. Phys., 2016. 18(44): p. 30696-30704.
https://www.ncbi.nlm.nih.gov/pubmed/27791210
nmrlearner
News from NMR blogs
0
01-25-2017 11:13 PM
Correction: Theory of solid effect and cross effect dynamic nuclear polarization with half-integer high-spin metal polarizing agents in rotating solids #DNPNMR
From The DNP-NMR Blog:
Correction: Theory of solid effect and cross effect dynamic nuclear polarization with half-integer high-spin metal polarizing agents in rotating solids #DNPNMR
p.p1 {margin: 0.0px 0.0px 0.0px 36.0px; text-indent: -36.0px; font: 12.0px Helvetica}
Corzilius, B., Correction: Theory of solid effect and cross effect dynamic nuclear polarization with half-integer high-spin metal polarizing agents in rotating solids. Phys. Chem. Chem. Phys., 2016. 18(42): p. 29643-29643.
http://dx.doi.org/10.1039/C6CP90249A
nmrlearner
News from NMR blogs
0
12-17-2016 07:18 AM
Theory of solid effect and cross effect dynamic nuclear polarization with half-integer high-spin metal polarizing agents in rotating solids #DNPNMR
From The DNP-NMR Blog:
Theory of solid effect and cross effect dynamic nuclear polarization with half-integer high-spin metal polarizing agents in rotating solids #DNPNMR
p.p1 {margin: 0.0px 0.0px 0.0px 36.0px; text-indent: -36.0px; font: 12.0px Helvetica}
Corzilius, B., Theory of solid effect and cross effect dynamic nuclear polarization with half-integer high-spin metal polarizing agents in rotating solids. Phys. Chem. Chem. Phys., 2016. 18(39): p. 27190-27204.
http://dx.doi.org/10.1039/C6CP04621E
nmrlearner
News from NMR blogs
0
11-21-2016 11:02 PM
Effects of the electron polarization on dynamic nuclear polarization in solids
From The DNP-NMR Blog:
Effects of the electron polarization on dynamic nuclear polarization in solids
Hovav, Y., et al., Effects of the electron polarization on dynamic nuclear polarization in solids. Phys Chem Chem Phys, 2015. 17(8): p. 6053-65.
http://www.ncbi.nlm.nih.gov/pubmed/25640165
Fast passage dynamic nuclear polarization on rotating solids
Fast passage dynamic nuclear polarization on rotating solids
November 2012
Publication year: 2012
Source:Journal of Magnetic Resonance, Volume 224</br>
</br>
Magic Angle Spinning (MAS) Dynamic Nuclear Polarization (DNP) has proven to be a very powerful way to improve the signal to noise ratio of NMR experiments on solids. The experiments have in general been interpreted considering the Solid-Effect (SE) and Cross-Effect (CE) DNP mechanisms while ignoring the influence of sample spinning. In this paper, we show experimental data of MAS-DNP enhancements of 1H and 13C in...
nmrlearner
Journal club
0
12-01-2012 06:10 PM
Fast Passage Dynamic Nuclear Polarization on Rotating Solids
Fast Passage Dynamic Nuclear Polarization on Rotating Solids
Publication year: 2012
Source:Journal of Magnetic Resonance</br>
Frederic Mentink-Vigier, Ümit Akbey, Yonatan Hovav, Shimon Vega, Hartmut Oschkinat, Akiva Feintuch</br>
Magic Angle Spinning (MAS) Dynamic Nuclear Polarization (DNP) has proven to be a very powerful way to improve the signal to noise ratio of NMR experiments on solids. The experiments have in general been interpreted considering the Solid-Effect (SE) and Cross-Effect (CE) DNP mechanisms while ignoring the influence of sample spinning. In this...
Electron Decoupling with Dynamic Nuclear Polarization in Rotating Solids #DNPNMR
Linear Mode
