Mathies, G., et al., Efficient Dynamic Nuclear Polarization at 800 MHz/527 GHz with Trityl-Nitroxide Biradicals. Angew Chem Int Ed Engl, 2015: p. n/a-n/a.
Cross-effect (CE) dynamic nuclear polarization (DNP) is a rapidly developing technique that enhances the signal intensities in magic-angle spinning (MAS) NMR spectra. We report CE DNP experiments at 211, 600, and 800 MHz using a new series of biradical polarizing agents referred to as TEMTriPols, in which a nitroxide (TEMPO) and a trityl radical are chemically tethered. The TEMTriPol molecule with the optimal performance yields a record 1 H NMR signal enhancement of 65 at 800 MHz at a concentration of 10 mM in a glycerol/water solvent matrix. The CE DNP enhancement for the TEMTriPol biradicals does not decrease as the magnetic field is increased in the manner usually observed for bis-nitroxides. Instead, the relatively strong exchange interaction between the trityl and nitroxide moieties determines the magnetic field at which the optimum enhancement is observed.
Cysteine-Specific Labeling of Proteins with a Nitroxide Biradical for Dynamic Nuclear Polarization NMR
From The DNP-NMR Blog:
Cysteine-Specific Labeling of Proteins with a Nitroxide Biradical for Dynamic Nuclear Polarization NMR
Voinov, M.A., et al., Cysteine-Specific Labeling of Proteins with a Nitroxide Biradical for Dynamic Nuclear Polarization NMR. J Phys Chem B, 2015. 119(32): p. 10180-90.
http://www.ncbi.nlm.nih.gov/pubmed/26230514
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08-24-2015 06:42 PM
[NMR paper] Cysteine-Specific Labeling of Proteins with a Nitroxide Biradical for Dynamic Nuclear Polarization NMR.
Cysteine-Specific Labeling of Proteins with a Nitroxide Biradical for Dynamic Nuclear Polarization NMR.
Related Articles Cysteine-Specific Labeling of Proteins with a Nitroxide Biradical for Dynamic Nuclear Polarization NMR.
J Phys Chem B. 2015 Jul 31;
Authors: Voinov MA, Good DB, Ward ME, Milikisiyants S, Marek A, Caporini MA, Rosay M, Munro RA, Ljumovic M, Brown LS, Ladizhansky V, Smirnov AI
Abstract
Dynamic nuclear polarization (DNP) enhances the signal in solid-state NMR of proteins by transferring polarization from...
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08-02-2015 07:10 AM
Self-assembled trityl radical capsules--implications for dynamic nuclear polarization
From The DNP-NMR Blog:
Self-assembled trityl radical capsules--implications for dynamic nuclear polarization
Marin-Montesinos, I., et al., Self-assembled trityl radical capsules--implications for dynamic nuclear polarization. Phys Chem Chem Phys, 2015. 17(8): p. 5785-94.
http://www.ncbi.nlm.nih.gov/pubmed/25626422
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04-29-2015 03:49 PM
High field dynamic nuclear polarization NMR with surfactant sheltered biradicals
From The DNP-NMR Blog:
High field dynamic nuclear polarization NMR with surfactant sheltered biradicals
Kiesewetter, M.K., et al., High field dynamic nuclear polarization NMR with surfactant sheltered biradicals. J Phys Chem B, 2014. 118(7): p. 1825-30.
http://www.ncbi.nlm.nih.gov/pubmed/24506193
Large Molecular Weight Nitroxide Biradicals Providing Efficient Dynamic Nuclear Polarization at Temperatures up to 200 K
From The DNP-NMR Blog:
Large Molecular Weight Nitroxide Biradicals Providing Efficient Dynamic Nuclear Polarization at Temperatures up to 200 K
Zagdoun, A., et al., Large Molecular Weight Nitroxide Biradicals Providing Efficient Dynamic Nuclear Polarization at Temperatures up to 200 K. J. Am. Chem. Soc., 2013. 135(34): p. 12790-12797.
http://dx.doi.org/10.1021/ja405813t
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09-25-2013 11:34 PM
Temperature dependence of high field 13C dynamic nuclear polarization processes with trityl radicals below 35 Kelvin
From The DNP-NMR Blog:
Temperature dependence of high field 13C dynamic nuclear polarization processes with trityl radicals below 35 Kelvin
Walker, S.A., et al., Temperature dependence of high field 13C dynamic nuclear polarization processes with trityl radicals below 35 Kelvin. Phys. Chem. Chem. Phys., 2013.
http://dx.doi.org/10.1039/C3CP51628H
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09-06-2013 06:52 PM
Large Molecular Weight Nitroxide Biradicals Providing Effi-cient Dynamic Nuclear Polarization at Temperatures up to 200 Kelvin
From The DNP-NMR Blog:
Large Molecular Weight Nitroxide Biradicals Providing Effi-cient Dynamic Nuclear Polarization at Temperatures up to 200 Kelvin
Zagdoun, A., et al., Large Molecular Weight Nitroxide Biradicals Providing Efficient Dynamic Nuclear Polarization at Temperatures up to 200 K. J Am Chem Soc, 2013. 135(34): p. 12790-7.
http://www.ncbi.nlm.nih.gov/pubmed/23961876
Efficient Dynamic Nuclear Polarization at 800 MHz/527 GHz with Trityl-Nitroxide Biradicals
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