Paioni, Alessandra Lucini, Marie A M Renault, and Marc Baldus. “DNP and Cellular Solid-State NMR,” 7:12, 2018.
https://onlinelibrary.wiley.com/doi/...590.emrstm1561.
Solid-state nuclear magnetic resonance (ssNMR) can provide structural information at the most detailed level and, at the same time, is applicable in highly heterogeneous and complex molecular environments, largely irrespective of solubility or crystallinity. Revolutionary developments in the field of dynamic nuclear polarization (DNP) have greatly enhanced ssNMR sensitivity. In this article, we discuss ssNMR concepts and applications that make use of these advancements and enable the study of complex biomolecular and even cellular systems at unprecedented structural resolution and molecular detail.
[NMR paper] Studying the assembly of the BAM complex in native membranes by cellular solid-state NMR spectroscopy.
Studying the assembly of the BAM complex in native membranes by cellular solid-state NMR spectroscopy.
Studying the assembly of the BAM complex in native membranes by cellular solid-state NMR spectroscopy.
J Struct Biol. 2017 Nov 29;:
Authors: Pinto C, Mance D, Julien M, Daniels M, Weingarth M, Baldus M
Abstract
Significant progress has been made in obtaining a structural insight into the assembly of the ?-barrel assembly machinery complex (BAM). These crystallography and electron microscopy studies used detergent as a membrane...
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12-05-2017 07:35 PM
[NMR paper] Efficient cellular solid-state NMR of membrane proteins by targeted protein labeling.
Efficient cellular solid-state NMR of membrane proteins by targeted protein labeling.
Related Articles Efficient cellular solid-state NMR of membrane proteins by targeted protein labeling.
J Biomol NMR. 2015 May 9;
Authors: Baker LA, Daniëls M, van der Cruijsen EA, Folkers GE, Baldus M
Abstract
Solid-state NMR spectroscopy (ssNMR) has made significant progress towards the study of membrane proteins in their native cellular membranes. However, reduced spectroscopic sensitivity and high background signal levels can complicate these...
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05-10-2015 07:49 PM
Efficient cellular solid-state NMR of membrane proteins by targeted protein labeling
Efficient cellular solid-state NMR of membrane proteins by targeted protein labeling
Abstract
Solid-state NMR spectroscopy (ssNMR) has made significant progress towards the study of membrane proteins in their native cellular membranes. However, reduced spectroscopic sensitivity and high background signal levels can complicate these experiments. Here, we describe a method for ssNMR to specifically label a single protein by repressing endogenous protein expression with rifampicin. Our results demonstrate that treatment of E. coli with rifampicin during...
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05-08-2015 09:18 PM
Cellular solid-state NMR investigation of a membrane protein using dynamic nuclear polarization
From The DNP-NMR Blog:
Cellular solid-state NMR investigation of a membrane protein using dynamic nuclear polarization
Yamamoto, K., et al., Cellular solid-state NMR investigation of a membrane protein using dynamic nuclear polarization. Biochimica et Biophysica Acta (BBA) - Biomembranes, 2015. 1848(1, Part B): p. 342-349.
http://dx.doi.org/10.1016/j.bbamem.2014.07.008
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05-04-2015 04:19 PM
[NMR paper] Cellular Solid-State NMR Investigation of a Membrane Protein Using Dynamic Nuclear Polarization.
Cellular Solid-State NMR Investigation of a Membrane Protein Using Dynamic Nuclear Polarization.
Related Articles Cellular Solid-State NMR Investigation of a Membrane Protein Using Dynamic Nuclear Polarization.
Biochim Biophys Acta. 2014 Jul 10;
Authors: Yamamoto K, Caporini MA, Im SC, Waskell L, Ramamoorthy A
Abstract
While an increasing number of structural biology studies successfully demonstrate the power of high-resolution structures and dynamics of membrane proteins in fully understanding their function, there is...
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07-16-2014 10:46 AM
Cellular Solid-State NMR Investigation of a Membrane Protein Using Dynamic Nuclear Polarization
Cellular Solid-State NMR Investigation of a Membrane Protein Using Dynamic Nuclear Polarization
Publication date: Available online 11 July 2014
Source:Biochimica et Biophysica Acta (BBA) - Biomembranes</br>
Author(s): Kazutoshi Yamamoto , Marc A. Caporini , Sang-Choul Im , Lucy Waskell , Ayyalusamy Ramamoorthy</br>
While an increasing number of structural biology studies successfully demonstrate the power of high-resolution structures and dynamics of membrane proteins in fully understanding their function, there is considerable interest in developing NMR...
DNP and Cellular Solid-state NMR #DNPNMR
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