Dissolution Dynamic Nuclear Polarization Methodology and Instrumentation #DNPNMR

		BioNMR > NMR community > News from NMR blogs
Dissolution Dynamic Nuclear Polarization Methodology and Instrumentation #DNPNMR

Webservers

NMR processing:

MDD

NMR assignment:

Backbone:

Side-chains:

NOEs:

Structure from NMR restraints:

Ab initio:

Fragment-based:

Rosetta-NMR (Robetta)

Template-based:

GeNMR

I-TASSER

Refinement:

Amber

Structure from chemical shifts:

Fragment-based:

Homology-based:

Torsion angles from chemical shifts:

Preditor

TALOS

Promega- Proline

Secondary structure from chemical shifts:

CSI (via RCI server)

TALOS

MICS caps, β-turns

d2D

PECAN

Flexibility from chemical shifts:

RCI

Interactions from chemical shifts:

HADDOCK

Chemical shifts re-referencing:

NMR model quality:

NOEs, other restraints:

Chemical shifts:

RDCs:

Pseudocontact shifts:

Protein geomtery:

SAVES2 or SAVES4

Quality Control Check

NMR spectrum prediction:

FANDAS

MestReS

V-NMR

Flexibility from structure:

Backbone S2

Methyl S2

B-factor

Molecular dynamics:

Gromacs

Amber

Antechamber

Chemical shifts prediction:

From structure:

Shiftx2

Sparta+

Camshift

CH3shift- Methyl

ArShift- Aromatic

ShiftS

Proshift

PPM

CheShift-2- Cα

From sequence:

Shifty

Camcoil

Poulsen_rc_CS

Disordered proteins:

MAXOCC

Format conversion & validation:

CCPN

From NMR-STAR 3.1

Validate NMR-STAR 3.1

NMR sample preparation:

Protein disorder:

DisMeta

Protein solubility:

Isotope labeling:

Solid-state NMR:

Thread Tools

Search this Thread

Rate Thread

Display Modes

06-16-2020, 01:36 AM

nmrlearner

Senior Member

Join Date: Jan 2005

Posts: 23,734

Points: 193,617, Level: 100

Level up: 0%, 0 Points needed

Activity: 50.7%

Last Achievements

Award-Showcase

NMR Credits: 0

NMR Points: 193,617

Downloads: 0

Uploads: 0

Dissolution Dynamic Nuclear Polarization Methodology and Instrumentation #DNPNMR

From The DNP-NMR Blog:

Dissolution Dynamic Nuclear Polarization Methodology and Instrumentation #DNPNMR

Kurzbach, Dennis, and Sami Jannin. “Dissolution Dynamic Nuclear Polarization Methodology and Instrumentation,” 7:16, 2018.

https://onlinelibrary.wiley.com/doi/...590.emrstm1563.

Dissolution dynamic nuclear polarization (d-DNP) denotes a method to enhance signals in nuclear magnetic resonance (NMR) spectroscopy by hyperpolarization of nuclear spins at cryogenic temperature in the solid state prior to a rapid dissolution, transfer of a sample to a conventional NMR spectrometer, and detection at ambient temperatures in the liquid state. The purpose of this chapter is to review the general methodology behind d-DNP, and instrumentational aspects going from basic tasks, such as sample preparation, over operational aspects, such as the use of crosspolarization techniques, magnetic tunnels, and the removal of polarization agents, to future perspectives, such as the long-distance transport of hyperpolarized substrates.

Go to The DNP-NMR Blog for more info.

Did you find this post helpful?

Similar Threads
Thread	Thread Starter	Forum	Replies	Last Post
Dipolar Order Mediated 1H -> 13C Cross-Polarization for Dissolution-Dynamic Nuclear Polarization #DNPNMR From The DNP-NMR Blog: Dipolar Order Mediated 1H -> 13C Cross-Polarization for Dissolution-Dynamic Nuclear Polarization #DNPNMR Elliott, Stuart J., Samuel F. Cousin, Quentin Chappuis, Olivier Cala, Morgan Ceillier, Aurélien Bornet, and Sami Jannin. “Dipolar Order Mediated 1H -> 13C Cross-Polarization for Dissolution-Dynamic Nuclear Polarization.” Preprint. Hyperpolarization/Pulse-sequence development, February 20, 2020. https://doi.org/10.5194/mr-2020-4	nmrlearner	News from NMR blogs	0	04-29-2020 04:16 PM
NMR Spectroscopy Unchained: Attaining the Highest Signal Enhancements in Dissolution Dynamic Nuclear Polarization #DNPNMR From The DNP-NMR Blog: NMR Spectroscopy Unchained: Attaining the Highest Signal Enhancements in Dissolution Dynamic Nuclear Polarization #DNPNMR Niedbalski, Peter, Andhika Kiswandhi, Christopher Parish, Qing Wang, Fatemeh Khashami, and Lloyd Lumata. “NMR Spectroscopy Unchained: Attaining the Highest Signal Enhancements in Dissolution Dynamic Nuclear Polarization.” The Journal of Physical Chemistry Letters 9, no. 18 (September 20, 2018): 5481–89. https://doi.org/10.1021/acs.jpclett.8b01687.	nmrlearner	News from NMR blogs	0	11-25-2018 06:02 AM
Instrumentation for cryogenic magic angle spinning dynamic nuclear polarization using 90L of liquid nitrogen per day #DNPNMR From The DNP-NMR Blog: Instrumentation for cryogenic magic angle spinning dynamic nuclear polarization using 90L of liquid nitrogen per day #DNPNMR p.p1 {margin: 0.0px 0.0px 0.0px 36.0px; text-indent: -36.0px; font: 12.0px Helvetica} Albert, B.J., et al., Instrumentation for cryogenic magic angle spinning dynamic nuclear polarization using 90L of liquid nitrogen per day. J. Magn. Reson., 2017. 283(Supplement C): p. 71-78. https://doi.org/10.1016/j.jmr.2017.08.014	nmrlearner	News from NMR blogs	0	12-20-2017 10:12 PM
Construction and 13 C hyperpolarization efficiency of a 180 GHz dissolution dynamic nuclear polarization system #DNPNMR From The DNP-NMR Blog: Construction and 13 C hyperpolarization efficiency of a 180 GHz dissolution dynamic nuclear polarization system #DNPNMR p.p1 {margin: 0.0px 0.0px 0.0px 36.0px; text-indent: -36.0px; font: 12.0px Helvetica} Kiswandhi, A., et al., Construction and 13 C hyperpolarization efficiency of a 180 GHz dissolution dynamic nuclear polarization system. Magn Reson Chem, 2017. 55(9): p. 828-836. https://www.ncbi.nlm.nih.gov/pubmed/28407455	nmrlearner	News from NMR blogs	0	10-14-2017 02:04 AM
Hyperpolarization of nitrogen-15 nuclei by cross polarization and dissolution dynamic nuclear polarization From The DNP-NMR Blog: Hyperpolarization of nitrogen-15 nuclei by cross polarization and dissolution dynamic nuclear polarization p.p1 {margin: 0.0px 0.0px 0.0px 36.0px; text-indent: -36.0px; font: 12.0px Helvetica} Milani, J., et al., Hyperpolarization of nitrogen-15 nuclei by cross polarization and dissolution dynamic nuclear polarization. Review of Scientific Instruments, 2017. 88(1): p. 015109. http://dx.doi.org/10.1063/1.4973777	nmrlearner	News from NMR blogs	0	09-04-2017 03:11 PM
Dissolution dynamic nuclear polarization–enhanced magnetic resonance spectroscopy and imaging: Chemical and biochemical reactions in nonequilibrium conditions #DNPNMR From The DNP-NMR Blog: Dissolution dynamic nuclear polarization–enhanced magnetic resonance spectroscopy and imaging: Chemical and biochemical reactions in nonequilibrium conditions #DNPNMR p.p1 {margin: 0.0px 0.0px 0.0px 36.0px; text-indent: -36.0px; font: 12.0px Helvetica} Lee, Y., Dissolution dynamic nuclear polarization–enhanced magnetic resonance spectroscopy and imaging: Chemical and biochemical reactions in nonequilibrium conditions. Applied Spectroscopy Reviews, 2015. 51(3): p. 210-226. https://doi.org/10.1080/05704928.2015.1116078	nmrlearner	News from NMR blogs	0	12-30-2016 04:53 PM
On The Potential of Dynamic Nuclear Polarization Enhanced Diamonds in Solid-State and Dissolution 13 C NMR Spectroscopy #DNPNMR From The DNP-NMR Blog: On The Potential of Dynamic Nuclear Polarization Enhanced Diamonds in Solid-State and Dissolution 13 C NMR Spectroscopy #DNPNMR Bretschneider, C.O., et al., On The Potential of Dynamic Nuclear Polarization Enhanced Diamonds in Solid-State and Dissolution 13 C NMR Spectroscopy. ChemPhysChem, 2016: p. n/a-n/a. http://www.ncbi.nlm.nih.gov/pubmed/27416769	nmrlearner	News from NMR blogs	0	08-26-2016 06:56 PM
Cross Polarization for Dissolution Dynamic Nuclear Polarization Experiments at Readily Accessible Temperatures 1.2* From The DNP-NMR Blog: Cross Polarization for Dissolution Dynamic Nuclear Polarization Experiments at Readily Accessible Temperatures 1.2*	nmrlearner	News from NMR blogs	0	11-21-2013 01:14 AM

« Previous Thread | Next Thread »

Posting Rules
You may not post new threads You may not post replies You may not post attachments You may not edit your posts BB code is On Smilies are On [IMG] code is On HTML code is Off Trackbacks are Off Pingbacks are Off Refbacks are Off