Direct enzyme-substrate affinity determination by real-time hyperpolarized (13)C-MRS

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Direct enzyme-substrate affinity determination by real-time hyperpolarized (13)C-MRS

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NMR processing:

MDD

NMR assignment:

Backbone:

Side-chains:

NOEs:

Structure from NMR restraints:

Ab initio:

Fragment-based:

Rosetta-NMR (Robetta)

Template-based:

GeNMR

I-TASSER

Refinement:

Amber

Structure from chemical shifts:

Fragment-based:

Homology-based:

Torsion angles from chemical shifts:

Preditor

TALOS

Promega- Proline

Secondary structure from chemical shifts:

CSI (via RCI server)

TALOS

MICS caps, β-turns

d2D

PECAN

Flexibility from chemical shifts:

RCI

Interactions from chemical shifts:

HADDOCK

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Methyl S2

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Chemical shifts prediction:

From structure:

Shiftx2

Sparta+

Camshift

CH3shift- Methyl

ArShift- Aromatic

ShiftS

Proshift

PPM

CheShift-2- Cα

From sequence:

Shifty

Camcoil

Poulsen_rc_CS

Disordered proteins:

MAXOCC

Format conversion & validation:

CCPN

From NMR-STAR 3.1

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NMR sample preparation:

Protein disorder:

DisMeta

Protein solubility:

Isotope labeling:

Solid-state NMR:

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10-29-2014, 03:51 PM

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Direct enzyme-substrate affinity determination by real-time hyperpolarized (13)C-MRS

From The DNP-NMR Blog:

Direct enzyme-substrate affinity determination by real-time hyperpolarized (13)C-MRS

Friesen-Waldner, L.J., et al., Direct enzyme-substrate affinity determination by real-time hyperpolarized (13)C-MRS. Chem Commun (Camb), 2014. 50(89): p. 13801-4.

http://www.ncbi.nlm.nih.gov/pubmed/25253534

A specialized kinetic analysis of real-time hyperpolarized [1,1,2,2-D4, 1-(13)C]choline (13)C-magnetic resonance spectroscopy enabled the determination of initial rates of metabolic enzyme activity (choline oxidase), enzyme-substrate affinity (Km), and inhibition. In a clinical MRI scanner, metabolite levels lower than 16 muM were detected at a temporal resolution of 1 s.

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