This is not an article about DNP-NMR spectroscopy, however, it deals with the measurements of temperatures in solid-state NMR experiments using KBR and referencing its chemical shift to 13C of adamantane in unstable magnetic fields.
Purusottam, R.N., G. Bodenhausen, and P. Tekely, Determination of sample temperature in unstable static fields by combining solid-state (79)Br and (13)C NMR. J Magn Reson, 2014. 246(0): p. 69-71.
Monitoring the isotropic chemical shifts to calibrate the sample temperature presupposes a perfect stability of the static magnetic field. It can be difficult to satisfy this requirement in solid-state NMR measurements. This paper describes a simple way to recover the accurate temperature dependence of the (79)Br resonance after subtracting changes of resonance frequency due to variations of the static field, monitored by the (13)C resonance.
[NMR paper] Resonance assignments of a membrane protein in phospholipid bilayers by combining multiple strategies of oriented sample solid-state NMR.
Resonance assignments of a membrane protein in phospholipid bilayers by combining multiple strategies of oriented sample solid-state NMR.
Related Articles Resonance assignments of a membrane protein in phospholipid bilayers by combining multiple strategies of oriented sample solid-state NMR.
J Biomol NMR. 2013 Dec 20;
Authors: Lu GJ, Opella SJ
Abstract
Oriented sample solid-state NMR spectroscopy can be used to determine the three-dimensional structures of membrane proteins in magnetically or mechanically aligned lipid bilayers. The...
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[NMR paper] Determination of structural topology of a membrane protein in lipid bilayers using polarization optimized experiments (POE) for static and MAS solid state NMR spectroscopy.
Determination of structural topology of a membrane protein in lipid bilayers using polarization optimized experiments (POE) for static and MAS solid state NMR spectroscopy.
Determination of structural topology of a membrane protein in lipid bilayers using polarization optimized experiments (POE) for static and MAS solid state NMR spectroscopy.
J Biomol NMR. 2013 Aug 21;
Authors: Mote KR, Gopinath T, Veglia G
Abstract
The low sensitivity inherent to both the static and magic angle spinning techniques of solid-state NMR (ssNMR) spectroscopy...
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08-23-2013 01:07 AM
Investigation of the Interface in Silica-Encapsulated Liposomes by Combining Solid State NMR and First Principles Calculations
Investigation of the Interface in Silica-Encapsulated Liposomes by Combining Solid State NMR and First Principles Calculations
Nicolas Folliet, Claire Roiland, Sylvie Be?gu, Anne Aubert, Tzonka Mineva, Annick Goursot, Kaliaperumal Selvaraj, Luminita Duma, Frederik Tielens, Francesco Mauri, Guillaume Laurent, Christian Bonhomme, Christel Gervais, Florence Babonneau and Thierry Azai?s
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Journal of the American Chemical Society
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Cryogenic temperature effects and resolution upon slow cooling of protein preparations in solid state NMR
Cryogenic temperature effects and resolution upon slow cooling of protein preparations in solid state NMR
Abstract X-ray crystallography using synchrotron radiation and the technique of dynamic nuclear polarization (DNP) in nuclear magnetic resonance (NMR) require samples to be kept at temperatures below 100 K. Protein dynamics are poorly understood below the freezing point of water and down to liquid nitrogen temperatures. Therefore, we investigate the α-spectrin SH3 domain by magic angle spinning (MAS) solid state NMR (ssNMR) at various temperatures while cooling slowly. Cooling down...
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08-13-2011 02:47 AM
[NMR paper] Low temperature solid-state NMR experiments of half-integer quadrupolar nuclides: cav
Low temperature solid-state NMR experiments of half-integer quadrupolar nuclides: caveats and data analysis.
Related Articles Low temperature solid-state NMR experiments of half-integer quadrupolar nuclides: caveats and data analysis.
J Magn Reson. 2004 May;168(1):66-74
Authors: Lipton AS, Heck RW, Sears JA, Ellis PD
Solid-state NMR spectroscopy of half-integer quadrupolar nuclides has received a lot of interest recently with the advent of new methodologies and higher magnetic fields. We present here the extension of our previous low...
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11-24-2010 09:51 PM
[NMR paper] Solid-state (13)C NMR reveals effects of temperature and hydration on elastin.
Solid-state (13)C NMR reveals effects of temperature and hydration on elastin.
Related Articles Solid-state (13)C NMR reveals effects of temperature and hydration on elastin.
Biophys J. 2002 Feb;82(2):1086-95
Authors: Perry A, Stypa MP, Tenn BK, Kumashiro KK
Elastin is the principal protein component of the elastic fiber in vertebrate tissue. The waters of hydration in the elastic fiber are believed to play a critical role in the structure and function of this largely hydrophobic, amorphous protein. (13)C CPMAS NMR spectra are acquired for...
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[NMR paper] Solid-state NMR studies of proteins: the view from static 2H NMR experiments.
Solid-state NMR studies of proteins: the view from static 2H NMR experiments.
Related Articles Solid-state NMR studies of proteins: the view from static 2H NMR experiments.
Biochem Cell Biol. 1998;76(2-3):411-22
Authors: Siminovitch DJ
The application of solid-state 2H NMR spectroscopy to the study of protein and peptide structure and dynamics is reviewed. The advantages of solid-state NMR for the study of proteins are considered, and the particular advantages of solid-state 2H NMR are summarized. Examples of work on the integral membrane...
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[NMR thesis] Solid state NMR at high magnetic fields using multiple pulse techniques
Solid state NMR at high magnetic fields using multiple pulse techniques
Carson, Douglas Glenn (1981) Solid state NMR at high magnetic fields using multiple pulse techniques. Dissertation (Ph.D.), California Institute of Technology. http://resolver.caltech.edu/CaltechETD:etd-10102006-095702
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Determination of sample temperature in unstable static fields by combining solid-state 79Br and 13C NMR
Linear Mode
