DAC-board based X-band EPR spectrometer with arbitrary waveform control

		BioNMR > NMR community > News from NMR blogs
DAC-board based X-band EPR spectrometer with arbitrary waveform control

Webservers

NMR processing:

MDD

NMR assignment:

Backbone:

Side-chains:

NOEs:

Structure from NMR restraints:

Ab initio:

Fragment-based:

Rosetta-NMR (Robetta)

Template-based:

GeNMR

I-TASSER

Refinement:

Amber

Structure from chemical shifts:

Fragment-based:

Homology-based:

Torsion angles from chemical shifts:

Preditor

TALOS

Promega- Proline

Secondary structure from chemical shifts:

CSI (via RCI server)

TALOS

MICS caps, β-turns

d2D

PECAN

Flexibility from chemical shifts:

RCI

Interactions from chemical shifts:

HADDOCK

Chemical shifts re-referencing:

NMR model quality:

NOEs, other restraints:

Chemical shifts:

RDCs:

Pseudocontact shifts:

Protein geomtery:

SAVES2 or SAVES4

Quality Control Check

NMR spectrum prediction:

FANDAS

MestReS

V-NMR

Flexibility from structure:

Backbone S2

Methyl S2

B-factor

Molecular dynamics:

Gromacs

Amber

Antechamber

Chemical shifts prediction:

From structure:

Shiftx2

Sparta+

Camshift

CH3shift- Methyl

ArShift- Aromatic

ShiftS

Proshift

PPM

CheShift-2- Cα

From sequence:

Shifty

Camcoil

Poulsen_rc_CS

Disordered proteins:

MAXOCC

Format conversion & validation:

CCPN

From NMR-STAR 3.1

Validate NMR-STAR 3.1

NMR sample preparation:

Protein disorder:

DisMeta

Protein solubility:

Isotope labeling:

Solid-state NMR:

Thread Tools

Search this Thread

Rate Thread

Display Modes

11-21-2013, 01:14 AM

nmrlearner

Senior Member

Join Date: Jan 2005

Posts: 23,732

Points: 193,617, Level: 100

Level up: 0%, 0 Points needed

Activity: 50.7%

Last Achievements

Award-Showcase

NMR Credits: 0

NMR Points: 193,617

Downloads: 0

Uploads: 0

DAC-board based X-band EPR spectrometer with arbitrary waveform control

From The DNP-NMR Blog:

DAC-board based X-band EPR spectrometer with arbitrary waveform control

Some instrumentation that is very interesting.

Kaufmann, T., et al., DAC-board based X-band EPR spectrometer with arbitrary waveform control. J Magn Reson, 2013. 235(0): p. 95-108.

http://www.ncbi.nlm.nih.gov/pubmed/23999530

<div style="text-align: justify;">We present arbitrary control over a homogenous spin system, demonstrated on a simple, home-built, electron paramagnetic resonance (EPR) spectrometer operating at 8-10 GHz (X-band) and controlled by a 1 GHz arbitrary waveform generator (AWG) with 42 dB (i.e. 14-bit) of dynamic range. Such a spectrometer can be relatively easily built from a single DAC (digital to analog converter) board with a modest number of stock components and offers powerful capabilities for automated digital calibration and correction routines that allow it to generate shaped X-band pulses with precise amplitude and phase control. It can precisely tailor the excitation profiles "seen" by the spins in the microwave resonator, based on feedback calibration with experimental input. We demonstrate the capability to generate a variety of pulse shapes, including rectangular, triangular, Gaussian, sinc, and adiabatic rapid passage waveforms. We then show how one can precisely compensate for the distortion and broadening caused by transmission into the microwave cavity in order to optimize corrected waveforms that are distinctly different from the initial, uncorrected waveforms. Specifically, we exploit a narrow EPR signal whose width is finer than the features of any distortions in order to map out the response to a short pulse, which, in turn, yields the precise transfer function of the spectrometer system. This transfer function is found to be consistent for all pulse shapes in the linear response regime. In addition to allowing precise waveform shaping capabilities, the spectrometer presented here offers complete digital control and calibration of the spectrometer that allows one to phase cycle the pulse phase with 0.007 degrees resolution and to specify the inter-pulse delays and pulse durations to

Did you find this post helpful?

Similar Threads
Thread	Thread Starter	Forum	Replies	Last Post
[NMR paper] Holistic Control of Herbal Teas and Tinctures Based on Sage (Salvia officinalis L.) for Compounds with Beneficial and Adverse Effects using NMR Spectroscopy. From Mendeley Biomolecular NMR group: Holistic Control of Herbal Teas and Tinctures Based on Sage (Salvia officinalis L.) for Compounds with Beneficial and Adverse Effects using NMR Spectroscopy. Analytical chemistry insights (2012). Pages: 1-12. Stephan G Walch, Dirk W Lachenmeier, Thomas Kuballa, Wolf Stühlinger, Yulia B Monakhova et al. A methodology that utilizes (1)H-NMR spectroscopy has been developed to simultaneously analyze toxic terpenes (thujone and camphor), major polyphenolic compounds, the total antioxidant capacity (ORAC) and the Folin-Ciocalteu (FC) index in foods...	nmrlearner	Journal club	0	10-17-2013 12:49 PM
Journal Highlight: NMR quadrupole liouvillians for arbitrary spin: Exact symbolic expressions and perturbation solutions Journal Highlight: NMR quadrupole liouvillians for arbitrary spin: Exact symbolic expressions and perturbation solutions http://www.spectroscopynow.com/common/images/thumbnails/13dc0c363d3.jpgThe NMR of quadrupolar nuclei with half-integer spins, which is described by treating the quadrupole interaction with second-order perturbation theory, has been elaborated on by calculating the transitions directly without the requirement of knowing the operators and wavefunctions. Read the rest at Spectroscopynow.com	nmrlearner	General	0	04-01-2013 04:23 PM
[NMR paper] Holistic Control of Herbal Teas and Tinctures Based on Sage (Salvia officinalis L.) for Compounds with Beneficial and Adverse Effects using NMR Spectroscopy. From Mendeley Biomolecular NMR group: Holistic Control of Herbal Teas and Tinctures Based on Sage (Salvia officinalis L.) for Compounds with Beneficial and Adverse Effects using NMR Spectroscopy. Analytical chemistry insights (2012). Pages: 1-12. Stephan G Walch, Dirk W Lachenmeier, Thomas Kuballa, Wolf Stühlinger, Yulia B Monakhova et al. A methodology that utilizes (1)H-NMR spectroscopy has been developed to simultaneously analyze toxic terpenes (thujone and camphor), major polyphenolic compounds, the total antioxidant capacity (ORAC) and the Folin-Ciocalteu (FC) index in foods...	nmrlearner	Journal club	0	08-24-2012 08:01 PM
Anin situelectrochemical cell for Q- and W-band EPR spectroscopy Anin situelectrochemical cell for Q- and W-band EPR spectroscopy Publication year: 2011 Source: Journal of Magnetic Resonance, Available online 22 September 2011</br> Paul R.Murray, DavidCollison, SimonDaff, NicolaAustin, Ruth*Edge, ...</br> A simple design for anin situ, three-electrode spectroelectrochemical cell is reported that can be used in commercial Q- and W-band (ca. 34 and 94 GHz, respectively) electron paramagnetic resonance (EPR) spectrometers, using standard sample tubing (1.0 and 0.5 mm inner diameter, respectively) and within variable temperature cryostat systems....	nmrlearner	Journal club	0	09-24-2011 06:04 AM
[Question from NMRWiki Q&A forum] Varian Gemini 300 Transmitter Board Indicator Lights Varian Gemini 300 Transmitter Board Indicator Lights I am having problems with a Varian Gemini 300 console. During the troubleshooting process, I noticed that the number of green lights activated on all of the transmitter boards has changed. In the schematic manual, these lights are labeled as XMTR, LOCK, OFF, and L0. However, I cannot find any more information as to what exactly these lights indicate. Can anyone help? Thanks in advance. Check if somebody has answered this question on NMRWiki QA forum	nmrlearner	News from other NMR forums	0	07-22-2011 10:39 PM
[NMR thesis] Band 3 structure and function : [superscript]35 C1 NMR and topographical investigatio Band 3 structure and function : 35 C1 NMR and topographical investigations Kanes, Katherine J. (1989) Band 3 structure and function : 35 C1 NMR and topographical investigations. Dissertation (Ph.D.), California Institute of Technology. http://resolver.caltech.edu/CaltechETD:etd-05302007-081241 More...	nmrlearner	NMR theses	0	08-27-2010 01:45 AM
[NMR paper] Source of transport site asymmetry in the band 3 anion exchange protein determined by Source of transport site asymmetry in the band 3 anion exchange protein determined by NMR measurements of external Cl- affinity. http://www.ncbi.nlm.nih.gov/corehtml/query/egifs/http:--pubs.acs.org-images-acspubs.jpg Related Articles Source of transport site asymmetry in the band 3 anion exchange protein determined by NMR measurements of external Cl- affinity. Biochemistry. 1996 Dec 3;35(48):15228-35 Authors: Liu D, Kennedy SD, Knauf PA Flux measurements indicate that a far greater number of unloaded band 3 anion transport sites face the...	nmrlearner	Journal club	0	08-22-2010 02:20 PM
Request a new message board here! Hello everybody! If you want to have a new BioNMR.com message board dedicated to a reasonably large "hot NMR topic" or a large piece of software (like CNS or NMRView), please post your request in a reply to this post. Thanks, Mark	administrator	BioNMR.com development	0	09-26-2006 06:46 PM

« Previous Thread | Next Thread »

Posting Rules
You may not post new threads You may not post replies You may not post attachments You may not edit your posts BB code is On Smilies are On [IMG] code is On HTML code is Off Trackbacks are Off Pingbacks are Off Refbacks are Off