Jawla, S., et al., Continuously Tunable 250 GHz Gyrotron with a Double Disk Window for DNP-NMR Spectroscopy. J Infrared Milli Terahz Waves, 2013. 34(1): p. 42-52.
In this paper, we describe the design and experimental results from the rebuild of a 250 GHz gyrotron used for Dynamic Nuclear Polarization enhanced Nuclear Magnetic Resonance spectroscopy on a 380 MHz spectrometer. Tuning bandwidth of approximately 2 GHz is easily achieved at a fixed magnetic field of 9.24 T and a beam current of 95 mA producing an average output power of >10 W over the entire tuning band. This tube incorporates a double disk output sapphire window in order to maximize the transmission at 250.58 GHz. DNP Signal enhancement of >125 is achieved on a 13C-Urea sample using this gyrotron.
αH(i,i+1) NOEs in helical peptide, can this be present continuously...
Hi,
I have observed some interesting NOEs for my peptide (5 kDa) in my 2D NOESY spectrum recorded at 900 MHz. I see NOEs that forms an α-helical pattern between 12-24 residues and N&C-terminus of my peptide is unstructured and does not have any secondary structure. The interesting part is, apart from seeing helical NOE patterns, such as αN(i,i+3)and αβ(i,i+3) for the residues between 12 & 24, I see in the alpha proton chemical shift regions of my 2D NOESY spectrum, a continuous αH(i,i+1) NOEs for the residues where I have helical secondary structural region (12-24 aa). That is, I see NOE...
Vivekanandan Subramanian
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05-06-2013 02:31 PM
Gyrotron DNP NMR, post-doc position - Telejob
Gyrotron DNP NMR, post-doc position
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Vom Mittwoch, 12. September 2012
Arbeitsort Lausanne
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EPFL enjoys a strong position in Dynamic Nuclear Polarization for NMR, because it has
developed original designs using several approaches. A campus-wide collaboration in
particular with the CRPP (fusion research center, specialized in gyrotrons) has allowed us to
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09-22-2012 09:38 PM
[Question from NMRWiki Q&A forum] edasp window for 1H/19F double resonance experiment
edasp window for 1H/19F double resonance experiment
Hello to all, This time I am having problem with doing a double resonance experiment. The final goal is to do 1H/19F-HOESY, but I am confused in the edasp window:http://dl.dropbox.com/u/12535433/04.JPG
I remember I was doing 1H/13C heteronuclear shift correlation experiments before. I think for 13C it uses X-transmitter(!) and x100W amplifier. A normal 1D-19F works fine (H50W-X-XBB).It is a NMR300 and a QNP probe.Thanks is advance,Amin
Check if somebody has answered this question on NMRWiki QA forum
[NMR paper] Structural studies of biomaterials using double-quantum solid-state NMR spectroscopy.
Structural studies of biomaterials using double-quantum solid-state NMR spectroscopy.
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Annu Rev Phys Chem. 2003;54:531-71
Authors: Drobny GP, Long JR, Karlsson T, Shaw W, Popham J, Oyler N, Bower P, Stringer J, Gregory D, Mehta M, Stayton PS
Proteins directly control the nucleation and growth of biominerals, but the details of molecular recognition at the protein-biomineral interface remain poorly understood. The elucidation of recognition...
Tunable paramagnetic relaxation enhancements by [Gd(DPA)3]3â?? for protein structure
Abstract Paramagnetic relaxation enhancements (PRE) present a powerful source of structural information in nuclear magnetic resonance (NMR) studies of proteins and proteinâ??ligand complexes. In contrast to conventional PRE reagents that are covalently attached to the protein, the complex between gadolinium and three dipicolinic acid (DPA) molecules, 3â??, can bind to proteins in a non-covalent yet site-specific manner. This offers straightforward access to PREs that can be scaled by using different ratios of 3â?? to protein, allowing quantitative distance measurements for nuclear spins...
Continuously Tunable 250 GHz Gyrotron with a Double Disk Window for DNP-NMR Spectroscopy
Linear Mode
