Communication: Dissolution DNP reveals a long-lived deuterium spin state imbalance in methyl groups #DNPNMR

		BioNMR > NMR community > News from NMR blogs
Communication: Dissolution DNP reveals a long-lived deuterium spin state imbalance in methyl groups #DNPNMR

Webservers

NMR processing:

MDD

NMR assignment:

Backbone:

Side-chains:

NOEs:

Structure from NMR restraints:

Ab initio:

Fragment-based:

Rosetta-NMR (Robetta)

Template-based:

GeNMR

I-TASSER

Refinement:

Amber

Structure from chemical shifts:

Fragment-based:

Homology-based:

Torsion angles from chemical shifts:

Preditor

TALOS

Promega- Proline

Secondary structure from chemical shifts:

CSI (via RCI server)

TALOS

MICS caps, β-turns

d2D

PECAN

Flexibility from chemical shifts:

RCI

Interactions from chemical shifts:

HADDOCK

Chemical shifts re-referencing:

NMR model quality:

NOEs, other restraints:

Chemical shifts:

RDCs:

Pseudocontact shifts:

Protein geomtery:

SAVES2 or SAVES4

Quality Control Check

NMR spectrum prediction:

FANDAS

MestReS

V-NMR

Flexibility from structure:

Backbone S2

Methyl S2

B-factor

Molecular dynamics:

Gromacs

Amber

Antechamber

Chemical shifts prediction:

From structure:

Shiftx2

Sparta+

Camshift

CH3shift- Methyl

ArShift- Aromatic

ShiftS

Proshift

PPM

CheShift-2- Cα

From sequence:

Shifty

Camcoil

Poulsen_rc_CS

Disordered proteins:

MAXOCC

Format conversion & validation:

CCPN

From NMR-STAR 3.1

Validate NMR-STAR 3.1

NMR sample preparation:

Protein disorder:

DisMeta

Protein solubility:

Isotope labeling:

Solid-state NMR:

Thread Tools

Search this Thread

Rate Thread

Display Modes

08-31-2017, 12:14 AM

nmrlearner

Senior Member

Join Date: Jan 2005

Posts: 23,777

Points: 193,617, Level: 100

Level up: 0%, 0 Points needed

Activity: 50.7%

Last Achievements

Award-Showcase

NMR Credits: 0

NMR Points: 193,617

Downloads: 0

Uploads: 0

Communication: Dissolution DNP reveals a long-lived deuterium spin state imbalance in methyl groups #DNPNMR

From The DNP-NMR Blog:

Communication: Dissolution DNP reveals a long-lived deuterium spin state imbalance in methyl groups #DNPNMR

p.p1 {margin: 0.0px 0.0px 0.0px 36.0px; text-indent: -36.0px; font: 12.0px Helvetica}
Jhajharia, A., et al., Communication: Dissolution DNP reveals a long-lived deuterium spin state imbalance in methyl groups. J Chem Phys, 2017. 146(4): p. 041101.

https://www.ncbi.nlm.nih.gov/pubmed/28147551

We report the generation and observation of long-lived spin states in deuterated methyl groups by dissolution DNP. These states are based on population imbalances between manifolds of spin states corresponding to irreducible representations of the C3v point group and feature strongly dampened quadrupolar relaxation. Their lifetime depends on the activation energies of methyl group rotation. With dissolution DNP, we can reduce the deuterium relaxation rate by a factor up to 20, thereby extending the experimentally available time window. The intrinsic limitation of NMR spectroscopy of quadrupolar spins by short relaxation times can thus be alleviated.
p.p1 {margin: 0.0px 0.0px 0.0px 0.0px; font: 12.0px Helvetica} p.p1 {margin: 0.0px 0.0px 0.0px 0.0px; font: 12.0px Helvetica}

Go to The DNP-NMR Blog for more info.

Did you find this post helpful?

Similar Threads
Thread	Thread Starter	Forum	Replies	Last Post
Magnetic resonance imaging of (1)H long lived states derived from parahydrogen induced polarization in a clinical system #DNPNMR From The DNP-NMR Blog: Magnetic resonance imaging of (1)H long lived states derived from parahydrogen induced polarization in a clinical system #DNPNMR Graafen, D., et al., Magnetic resonance imaging of (1)H long lived states derived from parahydrogen induced polarization in a clinical system. J Magn Reson, 2016. 262: p. 68-72. http://www.ncbi.nlm.nih.gov/pubmed/26754064	nmrlearner	News from NMR blogs	0	05-27-2016 06:18 PM
Long-lived spin States for low-field hyperpolarized gas MRI From The DNP-NMR Blog: Long-lived spin States for low-field hyperpolarized gas MRI Kovtunov, K.V., et al., Long-lived spin States for low-field hyperpolarized gas MRI. Chemistry, 2014. 20(45): p. 14629-32. http://www.ncbi.nlm.nih.gov/pubmed/25263795	nmrlearner	News from NMR blogs	0	02-13-2015 05:31 PM
Long-lived spin States for low-field hyperpolarized gas MRI From The DNP-NMR Blog: Long-lived spin States for low-field hyperpolarized gas MRI Kovtunov, K.V., et al., Long-lived spin States for low-field hyperpolarized gas MRI. Chemistry, 2014. 20(45): p. 14629-32. http://www.ncbi.nlm.nih.gov/pubmed/25263795	nmrlearner	News from NMR blogs	0	02-04-2015 09:57 PM
[NMR paper] Specific (13)C labeling of leucine, valine and isoleucine methyl groups for unambiguous detection of long-range restraints in protein solid-state NMR studies. Specific (13)C labeling of leucine, valine and isoleucine methyl groups for unambiguous detection of long-range restraints in protein solid-state NMR studies. Specific (13)C labeling of leucine, valine and isoleucine methyl groups for unambiguous detection of long-range restraints in protein solid-state NMR studies. J Magn Reson. 2015 Jan 6;252C:10-19 Authors: Fasshuber HK, Demers JP, Chevelkov V, Giller K, Becker S, Lange A Abstract Here we present an isotopic labeling strategy to easily obtain unambiguous long-range...	nmrlearner	Journal club	0	01-28-2015 05:28 PM
Specific 13C labeling of leucine, valine and isoleucine methyl groups for unambiguous detection of long-range restraints in protein solid-state NMR studies Specific 13C labeling of leucine, valine and isoleucine methyl groups for unambiguous detection of long-range restraints in protein solid-state NMR studies Publication date: Available online 6 January 2015 Source:Journal of Magnetic Resonance</br> Author(s): Hannes Klaus Fasshuber , Jean-Philippe Demers , Veniamin Chevelkov , Karin Giller , Stefan Becker , Adam Lange</br> Here we present an isotopic labeling strategy to easily obtain unambiguous long-range distance restraints in protein solid-state NMR studies. The method is based on the inclusion of two...	nmrlearner	Journal club	0	01-07-2015 11:26 AM
Long-Lived States of Magnetically Equivalent Spins Populated by Dissolution-DNP and Revealed by Enzymatic Reactions From The DNP-NMR Blog: Long-Lived States of Magnetically Equivalent Spins Populated by Dissolution-DNP and Revealed by Enzymatic Reactions Bornet, A., et al., Long-Lived States of Magnetically Equivalent Spins Populated by Dissolution-DNP and Revealed by Enzymatic Reactions. Chemistry, 2014. 20(51): p. 17113-8. http://www.ncbi.nlm.nih.gov/pubmed/25346515	nmrlearner	News from NMR blogs	0	12-17-2014 09:43 PM
Long-lived spin state of a tripeptide in stretched hydrogel Long-lived spin state of a tripeptide in stretched hydrogel Abstract The longitudinal (T 1), transverse (T 2), and singlet state (T s) relaxation times of the geminal backbone protons (CH2) of l-Leu-Gly-Gly were studied by NMR spectroscopy at 9.4 T in a bovine hide gelatin gel composed in D2O at 25Â*Â°C. Gelatin granules were dissolved in a hot solution of the tripeptide and then the solution was allowed to gel inside a flexible silicone tubing. With increases in gelatin content, the T...	nmrlearner	Journal club	0	06-19-2014 10:21 PM
Long-lived nuclear spin states in methyl groups and quantum-rotor-induced polarization From The DNP-NMR Blog: Long-lived nuclear spin states in methyl groups and quantum-rotor-induced polarization Meier, B., et al., Long-lived nuclear spin states in methyl groups and quantum-rotor-induced polarization. J Am Chem Soc, 2013. 135(50): p. 18746-9. http://www.ncbi.nlm.nih.gov/pubmed/24252212	nmrlearner	News from NMR blogs	0	02-13-2014 01:42 AM

« Previous Thread | Next Thread »

Posting Rules
You may not post new threads You may not post replies You may not post attachments You may not edit your posts BB code is On Smilies are On [IMG] code is On HTML code is Off Trackbacks are Off Pingbacks are Off Refbacks are Off