Characterization and optimization of the visualization performance of continuous flow overhauser DNP hyperpolarized water MRI: Inversion recovery approach
Characterization and optimization of the visualization performance of continuous flow overhauser DNP hyperpolarized water MRI: Inversion recovery approach
Terekhov, M., et al., Characterization and optimization of the visualization performance of continuous flow overhauser DNP hyperpolarized water MRI: Inversion recovery approach. Magn Reson Med, 2015: p. n/a-n/a.
PURPOSE: Overhauser dynamic nuclear polarization (DNP) allows the production of liquid hyperpolarized substrate inside the MRI magnet bore as well as its administration in continuous flow mode to acquire MR images with enhanced signal-to-noise ratio. We implemented inversion recovery preparation in order to improve contrast-to-noise ratio and to quantify the overall imaging performance of Overhauser DNP-enhanced MRI. METHOD: The negative enhancement created by DNP in combination with inversion recovery (IR) preparation allows canceling selectively the signal originated from Boltzmann magnetization and visualizing only hyperpolarized fluid. The theoretical model describing gain of MR image intensity produced by steady-state continuous flow DNP hyperpolarized magnetization was established and proved experimentally. RESULTS: A precise quantification of signal originated purely from DNP hyperpolarization was achieved. A temperature effect on longitudinal relaxation had to be taken into account to fit experimental results with numerical prediction. CONCLUSION: Using properly adjusted IR preparation, the complete zeroing of thermal background magnetization was achieved, providing an essential increase of contrast-to-noise ratio of DNP-hyperpolarized water images. To quantify and optimize the steady-state conditions for MRI with continuous flow DNP, an approach similar to that incorporating transient-state thermal magnetization equilibrium in spoiled fast field echo imaging sequences can be used. Magn Reson Med, 2015. (c) 2015 Wiley Periodicals, Inc.
Rationalizing Overhauser DNP of nitroxide radicals in water through MD simulations
From The DNP-NMR Blog:
Rationalizing Overhauser DNP of nitroxide radicals in water through MD simulations
Sezer, D., Rationalizing Overhauser DNP of nitroxide radicals in water through MD simulations. Phys Chem Chem Phys, 2014. 16(3): p. 1022-32.
http://www.ncbi.nlm.nih.gov/pubmed/24284869
nmrlearner
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01-31-2014 06:44 PM
Host-Guest Complexes as Water-Soluble High-Performance DNP Polarizing Agents
From The DNP-NMR Blog:
Host-Guest Complexes as Water-Soluble High-Performance DNP Polarizing Agents
Mao, J., et al., Host-Guest Complexes as Water-Soluble High-Performance DNP Polarizing Agents. J Am Chem Soc, 2013. 135(51): p. 19275-81.
http://www.ncbi.nlm.nih.gov/pubmed/24279469
nmrlearner
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01-10-2014 06:01 PM
[Question from NMRWiki Q&A forum] How to convert inversion recovery data acquired using Spinsight
How to convert inversion recovery data acquired using Spinsight
Hi,
I do not know how to convert a data set acquired using Spinsight into a format that can be read by Topspin. The data set is an inversion recovery data set (set of 1d FIDs with different relaxation (tau) delays so I think this is called a pseudo-2D data set). I know NMRPipe is probably the way to go, but I do not know where I can find any useful scripts or how to go about converting this dataset. Any advice is appreciated.
Mike.
nmrlearner
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11-15-2013 12:34 AM
1H-15N HSQC, edited by a 1H inversion recovery and observed in the antiphase component (IR-HSQC-AP)
Could someone explain the experimental theory/basis behind 1H-15N inversion recovery filtered HSQC experiment observed in antiphase (IR-HSQC-AP)? I am still learning basic NMR theory, but would like to know about this particular experiment, which was used to detect paramagnetically broadened backbone resonances. Thanks.
Abstract
Biogenesis of iron–sulfur cluster proteins is a highly regulated process that requires complex protein machineries. In the cytosolic iron–sulfur protein assembly machinery, two human key proteins—NADPH-dependent diflavin oxidoreductase 1 (Ndor1) and...
[NMR paper] The inter-ligand Overhauser effect: a powerful new NMR approach for mapping structura
The inter-ligand Overhauser effect: a powerful new NMR approach for mapping structural relationships of macromolecular ligands.
Related Articles The inter-ligand Overhauser effect: a powerful new NMR approach for mapping structural relationships of macromolecular ligands.
J Biomol NMR. 1999 Sep;15(1):71-6
Authors: Li D, DeRose EF, London RE
NMR experiments that transfer conformational information from the bound to the uncomplexed state via exchange have been utilized for many years. It is demonstrated here that inter-ligand NOEs ('ILOEs'),...
Characterization and optimization of the visualization performance of continuous flow overhauser DNP hyperpolarized water MRI: Inversion recovery approach
Linear Mode
