Enzymes are used as environmentally friendly catalysts in many industrial applications, and are frequently immobilized in a matrix to improve their chemical stability for long-term storage and reusability. Recently, it was shown that an atomic-level description of proteins immobilized in a biosilica matrix can be attained by examining their magic-angle spinning (MAS) NMR spectra. However, even though MAS NMR is an excellent tool for determining structure, it is severely hampered by sensitivity. In this work we provide the proof of principle that NMR characterization of biosilica-entrapped enzymes could be assisted by high-field dynamic nuclear polarization (DNP).
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Dissolution dynamic nuclear polarization–enhanced magnetic resonance spectroscopy and imaging: Chemical and biochemical reactions in nonequilibrium conditions #DNPNMR
From The DNP-NMR Blog:
Dissolution dynamic nuclear polarization–enhanced magnetic resonance spectroscopy and imaging: Chemical and biochemical reactions in nonequilibrium conditions #DNPNMR
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Lee, Y., Dissolution dynamic nuclear polarization–enhanced magnetic resonance spectroscopy and imaging: Chemical and biochemical reactions in nonequilibrium conditions. Applied Spectroscopy Reviews, 2015. 51(3): p. 210-226.
https://doi.org/10.1080/05704928.2015.1116078
Dynamic Nuclear Polarization Enhanced MAS NMR Spectroscopy for Structural Analysis of HIV-1 Protein Assemblies #DNPNMR
From The DNP-NMR Blog:
Dynamic Nuclear Polarization Enhanced MAS NMR Spectroscopy for Structural Analysis of HIV-1 Protein Assemblies #DNPNMR
Gupta, R., et al., Dynamic Nuclear Polarization Enhanced MAS NMR Spectroscopy for Structural Analysis of HIV-1 Protein Assemblies. J Phys Chem B, 2016. 120(2): p. 329-39.
http://www.ncbi.nlm.nih.gov/pubmed/26709853
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05-25-2016 02:33 PM
Dynamic Nuclear Polarization enhanced NMR at 187GHz/284MHz using an Extended Interaction Klystron amplifier #DNPNMR
From The DNP-NMR Blog:
Dynamic Nuclear Polarization enhanced NMR at 187GHz/284MHz using an Extended Interaction Klystron amplifier #DNPNMR
Kemp, T.F., et al., Dynamic Nuclear Polarization enhanced NMR at 187GHz/284MHz using an Extended Interaction Klystron amplifier. J Magn Reson, 2016. 265: p. 77-82.
http://www.ncbi.nlm.nih.gov/pubmed/26867091
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05-18-2016 09:53 PM
[NMR paper] Biosilica-Entrapped Enzymes Studied by Using Dynamic Nuclear-Polarization-Enhanced High-Field NMR Spectroscopy.
Biosilica-Entrapped Enzymes Studied by Using Dynamic Nuclear-Polarization-Enhanced High-Field NMR Spectroscopy.
Biosilica-Entrapped Enzymes Studied by Using Dynamic Nuclear-Polarization-Enhanced High-Field NMR Spectroscopy.
Chemphyschem. 2015 Aug 12;
Authors: Ravera E, Michaelis VK, Ong TC, Keeler EG, Martelli T, Fragai M, Griffin RG, Luchinat C
Abstract
Enzymes are used as environmentally friendly catalysts in many industrial applications, and are frequently immobilized in a matrix to improve their chemical stability for...
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08-13-2015 02:00 PM
[NMR paper] NMR-based structural biology enhanced by dynamic nuclear polarization at high magnetic field.
NMR-based structural biology enhanced by dynamic nuclear polarization at high magnetic field.
http://www.bionmr.com//www.ncbi.nlm.nih.gov/corehtml/query/egifs/http:--production.springer.de-OnlineResources-Logos-springerlink.gif Related Articles NMR-based structural biology enhanced by dynamic nuclear polarization at high magnetic field.
J Biomol NMR. 2014 Nov;60(2-3):157-68
Authors: Koers EJ, van der Cruijsen EA, Rosay M, Weingarth M, Prokofyev A, Sauvée C, Ouari O, van der Zwan J, Pongs O, Tordo P, Maas WE, Baldus M
Abstract
...
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06-24-2015 01:08 PM
NMR-based structural biology enhanced by dynamic nuclear polarization at high magnetic field
From The DNP-NMR Blog:
NMR-based structural biology enhanced by dynamic nuclear polarization at high magnetic field
Koers, E.J., et al., NMR-based structural biology enhanced by dynamic nuclear polarization at high magnetic field. J Biomol NMR, 2014. 60(2-3): p. 157-68.
http://www.ncbi.nlm.nih.gov/pubmed/25284462
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11-19-2014 04:32 PM
NMR-based structural biology enhanced by dynamic nuclear polarization at high magnetic field
NMR-based structural biology enhanced by dynamic nuclear polarization at high magnetic field
Abstract
Dynamic nuclear polarization (DNP) has become a powerful method to enhance spectroscopic sensitivity in the context of magnetic resonance imaging and nuclear magnetic resonance spectroscopy. We show that, compared to DNP at lower field (400Â*MHz/263Â*GHz), high field DNP (800Â*MHz/527Â*GHz) can significantly enhance spectral resolution and allows exploitation of the paramagnetic relaxation properties of DNP polarizing agents as direct structural...
Biosilica-Entrapped Enzymes Studied by Using Dynamic Nuclear-Polarization-Enhanced High-Field NMR Spectroscopy #DNPNMR
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