Hilty, C. and S. Bowen, Applications of dynamic nuclear polarization to the study of reactions and reagents in organic and biomolecular chemistry. Organic & Biomolecular Chemistry, 2010. 8(15): p. 3361-3365.
Nuclear Magnetic Resonance (NMR) is an important spectroscopic tool for the identification and structural characterization of molecules in chemistry and biochemistry. The most significant limitation of NMR compared to other spectroscopies is its relatively low sensitivity, which thus often requires long measurement times or large amounts of sample. A way of increasing sensitivity of single scan NMR spectra by several orders of magnitude is through hyperpolarization of nuclear spins. Dynamic nuclear polarization allows hyperpolarization of most spins in small molecules encountered in chemistry and biochemistry. NMR spectra of small amounts of samples from natural source, or from chemical synthesis can readily be acquired. Perhaps more interestingly, the availability of the entire hyperpolarized NMR signal in one single scan allows the measurement of transient processes in real time, if applied together with a stopped-flow technique. Through observation of chemical shift, different reactant and product species can be distinguished, and kinetics and mechanisms, for example in enzyme catalyzed reactions, can be elucidated. Real-time hyperpolarization-enhanced NMR is uniquely amenable to correlating atomic positions not only through space, but also over time between reactant and product species. Such correlations carry mechanistic information about a reaction, and can prove reaction pathways. Applications of this technique are emerging in different areas of chemistry concerned with rapid reactions, including not only enzymatic processes, but also chemical catalysis and protein folding.
Analysis of sensitivity enhancement by dynamic nuclear polarization in solid-state NMR: a case study of functionalized mesoporous materials
From The DNP-NMR Blog:
Analysis of sensitivity enhancement by dynamic nuclear polarization in solid-state NMR: a case study of functionalized mesoporous materials
Kobayashi, T., et al., Analysis of sensitivity enhancement by dynamic nuclear polarization in solid-state NMR: a case study of functionalized mesoporous materials. Phys Chem Chem Phys, 2013. 15(15): p. 5553-62.
http://www.ncbi.nlm.nih.gov/pubmed/23459985
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04-17-2013 08:15 PM
Analysis of sensitivity enhancement by dynamic nuclear polarization in solid-state NMR: a case study of functionalized mesoporous materials
From the The DNP-NMR Blog:
Analysis of sensitivity enhancement by dynamic nuclear polarization in solid-state NMR: a case study of functionalized mesoporous materials
Kobayashi, T., et al., Analysis of sensitivity enhancement by dynamic nuclear polarization in solid-state NMR: a case study of functionalized mesoporous materials. Phys Chem Chem Phys, 2013. 15(15): p. 5553-62.
http://www.ncbi.nlm.nih.gov/pubmed/23459985
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04-15-2013 08:52 AM
Dynamic Nuclear Polarization Study of Inhibitor Binding to the M218-60 Proton Transporter from Influenza A
From the The DNP-NMR Blog:
Dynamic Nuclear Polarization Study of Inhibitor Binding to the M218-60 Proton Transporter from Influenza A
Andreas, L.B., et al., Dynamic Nuclear Polarization Study of Inhibitor Binding to the M218-60 Proton Transporter from Influenza A. Biochemistry, 2013.
http://www.ncbi.nlm.nih.gov/pubmed/23480101
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04-15-2013 08:52 AM
Rapid Injection NMR Reveals ?3 ‘?-Allyl’ CuIII Intermediates in Addition Reactions of Organocuprate Reagents
Rapid Injection NMR Reveals ?3 ‘?-Allyl’ CuIII Intermediates in Addition Reactions of Organocuprate Reagents
Steven H. Bertz, Richard A. Hardin, Michael D. Murphy, Craig A. Ogle, Joshua D. Richter and Andy A. Thomas
http://pubs.acs.org/appl/literatum/publisher/achs/journals/content/jacsat/0/jacsat.ahead-of-print/ja3022363/aop/images/medium/ja-2012-022363_0012.gif
Journal of the American Chemical Society
DOI: 10.1021/ja3022363
http://feeds.feedburner.com/~ff/acs/jacsat?d=yIl2AUoC8zA
http://feeds.feedburner.com/~r/acs/jacsat/~4/FjoEID-4z0g
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05-25-2012 07:14 PM
[NMR900 blog] Cover article in Organic & Biomolecular Chemistry
Cover article in Organic & Biomolecular Chemistry
J. Guimond-Tremblay, M.-C. Gagnon, J.-A. Pineault-Maltais, V. Turcotte, M. Auger, J.-F. Paquin, "Synthesis and properties of monofluorinated dimyristoylphosphatidylcholine derivatives: Potential fluorinated probes for the study of membrane topology," Organic and Biomolecular Chemistry 10 (2012) 1145-1148. (Cover Article) http://dx.doi.org/10.1039/c2ob06570c https://blogger.googleusercontent.com/tracker/8663203727601106205-5483999220580812850?l=nmr900.blogspot.com
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