This is great work showing off the capabilities of DNP to study 19F in biological samples.
Lu, Manman, Mingzhang Wang, Ivan V. Sergeyev, Caitlin M Quinn, Jochem Struppe, Melanie Rosay, Werner Maas, Angela M. Gronenborn, and Tatyana Polenova. “19F Dynamic Nuclear Polarization at Fast Magic Angle Spinning for NMR of HIV-1 Capsid Protein Assemblies.” Journal of the American Chemical Society, March 15, 2019.
We report remarkably high, up to 100-fold, signal enhancements in 19F dynamic nuclear polarization (DNP) magic angle spinning (MAS) spectra at 14.1 T on HIV-1 CA capsid protein assemblies. These enhancements correspond to absolute sensitivity ratios of 12-29 and are of similar magnitude as seen for 1H signals in the same samples. At MAS frequencies above 20 kHz, it was possible to record 2D 19F-13C HETCOR spectra, which contain long-range intra- and intermolecular correlations. Such correlations provide unique distance restraints, inaccessible in conventional experiments without DNP for protein structure determination. Furthermore, systematic quantification of the DNP enhancements as a function of biradical concentration, MAS frequency, temperature, and microwave power is reported. Our work establishes the power of DNP-enhanced 19F MAS NMR spectroscopy for structural characterization of HIV-1 CA assemblies and this approach is anticipated to be applicable to a wide range of large biomolecular systems.
Dynamic Nuclear Polarization-Enhanced Biomolecular NMR Spectroscopy at High Magnetic Field with Fast Magic-Angle Spinning #DNPNMR
From The DNP-NMR Blog:
Dynamic Nuclear Polarization-Enhanced Biomolecular NMR Spectroscopy at High Magnetic Field with Fast Magic-Angle Spinning #DNPNMR
Jaudzems, Kristaps, Andrea Bertarello, Sachin R. Chaudhari, Andrea Pica, Diane Cala-De Paepe, Emeline Barbet-Massin, Andrew J. Pell, et al. “Dynamic Nuclear Polarization-Enhanced Biomolecular NMR Spectroscopy at High Magnetic Field with Fast Magic-Angle Spinning.” Angewandte Chemie 0 (2018).
https://doi.org/10.1002/ange.201801016.
[NMR paper] Dynamic nuclear polarization enhanced biomolecular NMR spectroscopy at high magnetic field with fast magic-angle spinning.
Dynamic nuclear polarization enhanced biomolecular NMR spectroscopy at high magnetic field with fast magic-angle spinning.
Dynamic nuclear polarization enhanced biomolecular NMR spectroscopy at high magnetic field with fast magic-angle spinning.
Angew Chem Int Ed Engl. 2018 Mar 22;:
Authors: Jaudzems K, Bertarello A, Chaudhari SR, Pica A, Cala-De Paepe D, Barbet-Massin E, Pell AJ, Akopjana I, Kotelovica S, Gajan D, Ouari O, Tars K, Pintacuda G, Lesage A
Abstract
Dynamic nuclear polarization (DNP) represents a powerful way to...
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03-23-2018 11:18 AM
Instrumentation for cryogenic magic angle spinning dynamic nuclear polarization using 90L of liquid nitrogen per day #DNPNMR
From The DNP-NMR Blog:
Instrumentation for cryogenic magic angle spinning dynamic nuclear polarization using 90L of liquid nitrogen per day #DNPNMR
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Albert, B.J., et al., Instrumentation for cryogenic magic angle spinning dynamic nuclear polarization using 90L of liquid nitrogen per day. J. Magn. Reson., 2017. 283(Supplement C): p. 71-78.
https://doi.org/10.1016/j.jmr.2017.08.014
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12-20-2017 10:12 PM
Dynamic nuclear polarization at 40 kHz magic angle spinning #DNPNMR
From The DNP-NMR Blog:
Dynamic nuclear polarization at 40 kHz magic angle spinning #DNPNMR
Chaudhari, S.R., et al., Dynamic nuclear polarization at 40 kHz magic angle spinning. Phys Chem Chem Phys, 2016. 18(15): p. 10616-22.
http://www.ncbi.nlm.nih.gov/pubmed/27035630
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News from NMR blogs
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08-23-2016 01:02 AM
Instrumentation for solid-state dynamic nuclear polarization with magic angle spinning NMR
From The DNP-NMR Blog:
Instrumentation for solid-state dynamic nuclear polarization with magic angle spinning NMR
Rosay, M., M. Blank, and F. Engelke, Instrumentation for solid-state dynamic nuclear polarization with magic angle spinning NMR. J Magn Reson, 2016. 264: p. 88-98.
http://www.ncbi.nlm.nih.gov/pubmed/26920834
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News from NMR blogs
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04-18-2016 03:17 PM
Theoretical Aspects of Magic Angle Spinning - Dynamic Nuclear Polarization
From The DNP-NMR Blog:
Theoretical Aspects of Magic Angle Spinning - Dynamic Nuclear Polarization
Mentink-Vigier, F., et al., Theoretical Aspects of Magic Angle Spinning - Dynamic Nuclear Polarization. J. Magn. Reson., 2015.
http://www.sciencedirect.com/science/article/pii/S1090780715001500
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News from NMR blogs
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07-27-2015 10:41 PM
[NMR paper] Magic Angle Spinning NMR Reveals Sequence-Dependent Structural Plasticity, Dynamics, and the Spacer Peptide 1 Conformation in HIV-1 Capsid Protein Assemblies.
Magic Angle Spinning NMR Reveals Sequence-Dependent Structural Plasticity, Dynamics, and the Spacer Peptide 1 Conformation in HIV-1 Capsid Protein Assemblies.
Magic Angle Spinning NMR Reveals Sequence-Dependent Structural Plasticity, Dynamics, and the Spacer Peptide 1 Conformation in HIV-1 Capsid Protein Assemblies.
J Am Chem Soc. 2013 Oct 28;
Authors: Han Y, Hou G, Suiter CL, Ahn J, Byeon IJ, Lipton AS, Burton SD, Hung I, Gor'kov PL, Gan Z, Brey WW, Rice D, Gronenborn AM, Polenova TE
Abstract
A key stage in HIV-1 maturation towards...
19F Dynamic Nuclear Polarization at Fast Magic Angle Spinning for NMR of HIV-1 Capsid Protein Assemblies #DNPNMR
Linear Mode
