The synthesis, NMR properties and preliminary polarization tests on protonated and perdeuterated forms of alpha-trimethylglutamine (NMe3Gln), alpha-trimethylglutamate (NMe3Glu) and epsilon-trimethyllysine (NMe3Lys) are reported. The (15)N-permethylated, perdeuterated amino acids display very long (15)N-T1 values, ranging between 190 and 330 s, are well polarized by the dynamic nuclear polarization (DNP) procedure, yielding good polarization levels (10%), and appear to be well tolerated by cells and mice. The obtained results make perdeuterated amino acids excellent candidates for innovative DNP (15)N-MRI applications such as perfusion or targeting studies.
[NMR paper] Photo-CIDNP NMR Spectroscopy of Amino Acids and Proteins.
Photo-CIDNP NMR Spectroscopy of Amino Acids and Proteins.
Related Articles Photo-CIDNP NMR Spectroscopy of Amino Acids and Proteins.
Top Curr Chem. 2013 May 14;
Authors: Kuhn LT
Abstract
Photo-chemically induced dynamic nuclear polarization (CIDNP) is a nuclear magnetic resonance (NMR) phenomenon which, among other things, is exploited to extract information on biomolecular structure via probing solvent-accessibilities of tryptophan (Trp), tyrosine (Tyr), and histidine (His) amino acid side chains both in polypeptides and proteins in...
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05-15-2013 03:12 PM
[Question from NMRWiki Q&A forum] 13C quaternary centers in amino acids
13C quaternary centers in amino acids
I've got a sample of about 5mg of an amino acid that is the final product of a a synthesis. Due to the long relaxation time that the carboxylic and the alpha C we only got a 200 varian Mercury instrument and we're unable to obtain those signals. I was wondering if an APT is better than DEPT, because we're only interested in this signals and i've heard the overall pulse sequence is shorter than the DEPT, increasing the number of scans in the same period of time.
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09-01-2011 07:20 AM
[Question from NMRWiki Q&A forum] 13C cuaternary centers in amino acids
13C cuaternary centers in amino acids
I've got a sample of about 5mg of an amino acid that is the final product of a a synthesis. Due to the long relaxation time that the carboxilic and the alpha C we only got a 200 varian Mercury instrument and we're unable to obtain those signals. I was wondering if an APT is better than DEPT, because we're only interested in this signals and i've heart the overall pulse sequence is shorter than the DEPT, increasing the number of scans in the same period of time
Check if somebody has answered this question on NMRWiki QA forum
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08-31-2011 07:12 PM
[KPWU blog] Names of Atoms of Amino acids
Names of Atoms of Amino acids
I really hate the inconsistent nomenclature of atoms of amino acids between different programs/database. I finished all NOESY assignment on Sparky using PDB nomenclature and the Sparky XPLOR constraint plugin (shortcut xf) doesn’t take care of the differences between XPLOR and PDB. Thus I have to find a table showing me the differences of names http://stats.wordpress.com/b.gif?host=kpwu.wordpress.com&blog=76132&post=262&subd=kpwu&ref=&feed=1
Go to KPWU blog to read complete post.
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01-28-2011 04:52 AM
[NMR paper] A simple efficient synthesis of [23,24]-(13)C(2)-labeled bile salts as NMR probes of
A simple efficient synthesis of -(13)C(2)-labeled bile salts as NMR probes of protein-ligand interactions.
Related Articles A simple efficient synthesis of -(13)C(2)-labeled bile salts as NMR probes of protein-ligand interactions.
Bioorg Med Chem Lett. 2002 Feb 11;12(3):433-5
Authors: Tochtrop GP, DeKoster GT, Cistola DP, Covey DF
The synthesis of -(13)C(2)-labeled bile salts is achieved through a steroidal side chain degradation and isotopic regeneration strategy. Three common bile acids were degraded to the corresponding C(22 )aldehyde by an...
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11-24-2010 08:49 PM
[NMR paper] Modulation of intrinsic phi,psi propensities of amino acids by neighbouring residues
Modulation of intrinsic phi,psi propensities of amino acids by neighbouring residues in the coil regions of protein structures: NMR analysis and dissection of a beta-hairpin peptide.
Related Articles Modulation of intrinsic phi,psi propensities of amino acids by neighbouring residues in the coil regions of protein structures: NMR analysis and dissection of a beta-hairpin peptide.
J Mol Biol. 1998 Dec 18;284(5):1597-609
Authors: Griffiths-Jones SR, Sharman GJ, Maynard AJ, Searle MS
Analysis of residues in coil regions of protein structures...
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11-17-2010 11:15 PM
[NMR paper] The solution conformations of amino acids from molecular dynamics simulations of Gly-
The solution conformations of amino acids from molecular dynamics simulations of Gly-X-Gly peptides: comparison with NMR parameters.
Related Articles The solution conformations of amino acids from molecular dynamics simulations of Gly-X-Gly peptides: comparison with NMR parameters.
Biochem Cell Biol. 1998;76(2-3):164-70
Authors: van der Spoel D
The conformations that amino acids can adopt in the random coil state are of fundamental interest in the context of protein folding research and studies of protein-peptide interactions. To date, no...
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11-17-2010 11:06 PM
[NMR paper] Comparison between the phi distribution of the amino acids in the protein database an
Comparison between the phi distribution of the amino acids in the protein database and NMR data indicates that amino acids have various phi propensities in the random coil conformation.
http://www.ncbi.nlm.nih.gov/corehtml/query/egifs/http:--linkinghub.elsevier.com-ihub-images-PubMedLink.gif Related Articles Comparison between the phi distribution of the amino acids in the protein database and NMR data indicates that amino acids have various phi propensities in the random coil conformation.
J Mol Biol. 1995 Nov 24;254(2):322-33
Authors: Serrano L
...
15N-permethylated amino acids as efficient probes for MRI-DNP applications
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