Related ArticlesZooming into the Interaction of ?-synuclein with Calcium Ions through Exclusively Heteronuclear NMR Experiments.
Angew Chem Int Ed Engl. 2020 Jul 31;:
Authors: Pontoriero L, Schiavina M, Murrali MG, Pierattelli R, Felli IC
Abstract
Many properties of intrinsically disordered proteins (IDPs) or protein regions (IDRs) are expected to be modulated by the nature of amino acid side chains as well as by local solvent exposure. Here we propose a set of exclusively heteronuclear NMR experiments to investigate these features in different experimental conditions relevant for physiological function. The proposed approach is generally applicable to many IDPs/IDRs whose assignment is available in the BMRB to investigate how their properties are modulated by different, physiologically relevant conditions. The experiments are tested on ?-synuclein. They are then used to investigate how ?-synuclein senses Ca2+ * concentration jumps associated to the transmission of nervous signals. Novel modules in the primary sequence of ?-synuclein optimized for calcium sensing in highly flexible, disordered protein segments are identified.
PMID: 32735376 [PubMed - as supplied by publisher]
[ASAP] Comparative Analysis of the Conformation, Aggregation, Interaction, and Fibril Morphologies of Human a-, ß-, and ?-Synuclein Proteins
Comparative Analysis of the Conformation, Aggregation, Interaction, and Fibril Morphologies of Human a-, ß-, and ?-Synuclein Proteins
https://pubs.acs.org/appl/literatum/publisher/achs/journals/content/bichaw/0/bichaw.ahead-of-print/acs.biochem.8b00343/20180612/images/medium/bi-2018-00343q_0010.gif
Biochemistry
DOI: 10.1021/acs.biochem.8b00343
http://feeds.feedburner.com/~ff/acs/bichaw?d=yIl2AUoC8zA
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06-13-2018 05:09 PM
[NMR paper] NMR Observation of ?-Synuclein Membrane Interaction by Monitoring Acetylation Reactivity of its Lysine Sidechains.
NMR Observation of ?-Synuclein Membrane Interaction by Monitoring Acetylation Reactivity of its Lysine Sidechains.
Related Articles NMR Observation of ?-Synuclein Membrane Interaction by Monitoring Acetylation Reactivity of its Lysine Sidechains.
Biochemistry. 2016 Jul 25;
Authors: Lee JH, Ying J, Bax A
Abstract
The interaction between ?-synuclein (?S) protein and lipid membranes is key to its role in synaptic vesicle homeostasis, and plays a role in initiating fibril formation which is implicated in Parkinson's disease. The...
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07-28-2016 12:07 PM
Design of a hyperpolarized 15N NMR probe that induces a large chemical-shift change upon binding of calcium ions
From The DNP-NMR Blog:
Design of a hyperpolarized 15N NMR probe that induces a large chemical-shift change upon binding of calcium ions
Hata, R., et al., Design of a hyperpolarized 15N NMR probe that induces a large chemical-shift change upon binding of calcium ions. Chemical Communications, 2015. 51(61): p. 12290-12292.
http://dx.doi.org/10.1039/C5CC04597E
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08-07-2015 05:49 PM
[NMR paper] NMR Characterization of the Interaction of the Endonuclease Domain of MutL with Divalent Metal Ions and ATP.
NMR Characterization of the Interaction of the Endonuclease Domain of MutL with Divalent Metal Ions and ATP.
Related Articles NMR Characterization of the Interaction of the Endonuclease Domain of MutL with Divalent Metal Ions and ATP.
PLoS One. 2014;9(6):e98554
Authors: Mizushima R, Kim JY, Suetake I, Tanaka H, Takai T, Kamiya N, Takano Y, Mishima Y, Tajima S, Goto Y, Fukui K, Lee YH
Abstract
MutL is a multi-domain protein comprising an N-terminal ATPase domain (NTD) and C-terminal dimerization domain (CTD), connected with...
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06-06-2014 03:59 PM
[NMR paper] A molecular dynamics simulations-based interpretation of NMR multidimensional heteronuclear spectra of alpha-synuclein/dopamine adducts.
A molecular dynamics simulations-based interpretation of NMR multidimensional heteronuclear spectra of alpha-synuclein/dopamine adducts.
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Biochemistry. 2013 Aug 21;
Authors: Dibenedetto D, Rossetti G, Caliandro R, Carloni P
Abstract
Multidimensional heteronuclear NMR spectroscopy provides valuable structural information on adducts between naturally unfolded proteins and their ligands....
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08-24-2013 04:53 PM
[NMR paper] Site-specific interaction between ?-synuclein and membranes probed by NMR-observed methionine oxidation rates.
Site-specific interaction between ?-synuclein and membranes probed by NMR-observed methionine oxidation rates.
Related Articles Site-specific interaction between ?-synuclein and membranes probed by NMR-observed methionine oxidation rates.
J Am Chem Soc. 2013 Feb 11;
Authors: Maltsev AS, Chen J, Levine RL, Bax A
Abstract
?-Synuclein (aS) is an intrinsically disordered protein that is water soluble but also can bind negatively charged lipid membranes while adopting an ?-helical conformation. Membrane affinity is increased by...
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02-13-2013 12:47 PM
[NMR paper] Copper(I)-?-Synuclein Interaction: Structural Description of Two Independent and Competing Metal Binding Sites.
Copper(I)-?-Synuclein Interaction: Structural Description of Two Independent and Competing Metal Binding Sites.
http://www.ncbi.nlm.nih.gov/corehtml/query/egifs/http:--pubs.acs.org-images-pubmed-acspubs.jpg Related Articles Copper(I)-?-Synuclein Interaction: Structural Description of Two Independent and Competing Metal Binding Sites.
Inorg Chem. 2013 Jan 23;
Authors: Camponeschi F, Valensin D, Tessari I, Bubacco L, Dell'acqua S, Casella L, Monzani E, Gaggelli E, Valensin G
Abstract
The aggregation of ?-synuclein (?S) is a critical step in...
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02-03-2013 10:19 AM
[NMR paper] Phosphate ions in bone: identification of a calcium-organic phosphate complex by 31P
Phosphate ions in bone: identification of a calcium-organic phosphate complex by 31P solid-state NMR spectroscopy at early stages of mineralization.
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Calcif Tissue Int. 2003 May;72(5):610-26
Authors: Wu Y, Ackerman JL, Strawich ES, Rey C, Kim HM, Glimcher MJ
Previous 31P cross-polarization and differential cross-polarization magic angle spinning (CP/MAS and DCP/MAS) solid-state NMR...
Zooming into the Interaction of ?-synuclein with Calcium Ions through Exclusively Heteronuclear NMR Experiments.
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