Longitudinal exchange: an alternative strategy towards quantification of dynamics parameters in ZZ exchange spectroscopy
Longitudinal exchange: an alternative strategy towards quantification of dynamics parameters in ZZ exchange spectroscopy
Abstract Longitudinal exchange experiments facilitate the quantification of the rates of interconversion between the exchanging species, along with their longitudinal relaxation rates, by analyzing the time-dependence of direct correlation and exchange cross peaks. Here we present a simple and robust alternative to this strategy, which is based on the combination of two complementary experiments, one with and one without resolving exchange cross peaks. We show that...
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09-30-2011 08:01 PM
TROSY-selected ZZ-exchange experiment for characterizing slow chemical exchange in large proteins
TROSY-selected ZZ-exchange experiment for characterizing slow chemical exchange in large proteins
Abstract A TROSY-selected ZZ-exchange experiment is described for measuring slow chemical exchange rates by monitoring the TROSY component of 15N longitudinal magnetization. Application of the proposed pulse sequence to the cadherin 8 N-terminal extracelluar domain demonstrates that enhanced sensitivity is obtained, compared to a previously described TROSY-detected ZZ-exchange sequence (Sahu et al. J Am Chem Soc 129: 13232â??13237, 2007), by preserving the TROSY effect during the mixing...
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01-09-2011 12:46 PM
[NMR paper] A model of reversible inhibitors in the gastric H+/K+-ATPase binding site determined
A model of reversible inhibitors in the gastric H+/K+-ATPase binding site determined by rotational echo double resonance NMR.
Related Articles A model of reversible inhibitors in the gastric H+/K+-ATPase binding site determined by rotational echo double resonance NMR.
J Biol Chem. 2001 Nov 16;276(46):43197-204
Authors: Watts JA, Watts A, Middleton DA
Several close analogues of the noncovalent H(+)/K(+)-ATPase inhibitor SCH28080 (2-methyl-3-cyanomethyl-8-(phenylmethoxy)imidazopyridine) have been screened for activity and examined in the...
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11-19-2010 08:44 PM
[NMR paper] Reversible induction of ATP synthesis by DNA damage and repair in Escherichia coli. I
Reversible induction of ATP synthesis by DNA damage and repair in Escherichia coli. In vivo NMR studies.
Related Articles Reversible induction of ATP synthesis by DNA damage and repair in Escherichia coli. In vivo NMR studies.
J Biol Chem. 1998 Nov 13;273(46):30232-8
Authors: Dahan-Grobgeld E, Livneh Z, Maretzek AF, Polak-Charcon S, Eichenbaum Z, Degani H
Early metabolic events in Escherichia coli exposed to nalidixic acid, a topoisomerase II inhibitor and an inducer of the SOS system, were investigated by in vivo NMR spectroscopy, a technique...
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11-17-2010 11:15 PM
[NMR paper] Temperature-reversible eruptions of vesicles in model membranes studied by NMR.
Temperature-reversible eruptions of vesicles in model membranes studied by NMR.
http://www.ncbi.nlm.nih.gov/corehtml/query/egifs/http:--linkinghub.elsevier.com-ihub-images-cellhub.gif http://www.ncbi.nlm.nih.gov/corehtml/query/egifs/http:--www.pubmedcentral.nih.gov-corehtml-pmc-pmcgifs-pubmed-pmc.gif Related Articles Temperature-reversible eruptions of vesicles in model membranes studied by NMR.
Biophys J. 1992 May;61(5):1413-26
Authors: Nezil FA, Bayerl S, Bloom M
Deuterium (2H) and phosphorus (31P) nuclear magnetic resonance (NMR) and...
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08-21-2010 11:41 PM
[NMR paper] Reversible unfolding of cytochrome c upon interaction with cardiolipin bilayers. 2. E
Reversible unfolding of cytochrome c upon interaction with cardiolipin bilayers. 2. Evidence from phosphorus-31 NMR measurements.
Related Articles Reversible unfolding of cytochrome c upon interaction with cardiolipin bilayers. 2. Evidence from phosphorus-31 NMR measurements.
Biochemistry. 1991 Apr 23;30(16):3880-5
Authors: Spooner PJ, Watts A
31P NMR measurements were conducted to determine the structural and chemical environment of beef heart cardiolipin when bound to cytochrome c. 31P NMR line shapes infer that the majority of lipid remains...
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08-21-2010 11:16 PM
[NMR paper] Reversible unfolding of cytochrome c upon interaction with cardiolipin bilayers. 1. E
Reversible unfolding of cytochrome c upon interaction with cardiolipin bilayers. 1. Evidence from deuterium NMR measurements.
Related Articles Reversible unfolding of cytochrome c upon interaction with cardiolipin bilayers. 1. Evidence from deuterium NMR measurements.
Biochemistry. 1991 Apr 23;30(16):3871-9
Authors: Spooner PJ, Watts A
Deuterium NMR has been used to investigate the structure and dynamic state of cytochrome c complexed with bilayers of cardiolipin. Reductive methylation was employed to prepare lysyl cytochrome c, and deuterium...
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Elucidation of the structure and intermolecular interactions of a reversible cyclic-p
Elucidation of the structure and intermolecular interactions of a reversible cyclic-peptide inhibitor of the proteasome by NMR spectroscopy and molecular modeling.
http://www.ncbi.nlm.nih.gov/corehtml/query/egifs/http:--www3.interscience.wiley.com-aboutus-images-wiley_interscience_pubmed_logo_120x27.gif Related Articles Elucidation of the structure and intermolecular interactions of a reversible cyclic-peptide inhibitor of the proteasome by NMR spectroscopy and molecular modeling.
Angew Chem Int Ed Engl. 2010 May 25;49(23):3934-8
Authors: Stauch B, Simon...
Zero-Field NMR Enhancedby Parahydrogen in Reversible Exchange
Linear Mode
