In this study, the combined forms of Pb in Cladophora rupestris (L.) (C. rupestris) were investigated via X-ray photoelectron spectroscopy (XPS) and nuclear magnetic resonance (NMR), different Pb concentrations (0, 0.5, and 5.0 mg/L), and C. rupestris subcells were explored. Results showed that combined forms of Pb mainly account for Pb-polysaccharides (Pb-OH of carbohydrates) in the cell wall, Pb-protein (Pb-N= and (C-N-)(2)Pb) in the organelle, and Pb-organic acid (Pb-sulfates, (CO)(2)-Pb and...
Pressure control helps NMR analyze protein as it folds - Chemical & Engineering News
Pressure control helps NMR analyze protein as it folds - Chemical & Engineering News
Pressure control helps NMR analyze protein as it folds Chemical & Engineering NewsResearchers have long wanted to better understand the way an unfolded protein chain reaches its final folded structure. But experimental tools needed to probe ...
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01-07-2019 05:49 AM
[NMR paper] Backbone NMR assignments of tryparedoxin, the central protein in the hydroperoxide detoxification cascade of African trypanosomes, in the oxidized and reduced form.
Backbone NMR assignments of tryparedoxin, the central protein in the hydroperoxide detoxification cascade of African trypanosomes, in the oxidized and reduced form.
Related Articles Backbone NMR assignments of tryparedoxin, the central protein in the hydroperoxide detoxification cascade of African trypanosomes, in the oxidized and reduced form.
Biomol NMR Assign. 2017 Jun 01;:
Authors: Wagner A, Diehl E, Krauth-Siegel RL, Hellmich UA
Abstract
Tryparedoxin (Tpx) is a pivotal protein in the redox-metabolism of trypanosomatid...
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06-03-2017 11:49 AM
[NMR paper] DIADECOMP: A new approach to analyze decompositions from projection spectroscopy
DIADECOMP: A new approach to analyze decompositions from projection spectroscopy
Publication date: December 2016
Source:Journal of Magnetic Resonance, Volume 273</br>
Author(s): Jonas Fredriksson, Viviane S. de Paula, Ana Paula Valente, Fabio C.L. Almeida, Martin Billeter</br>
We demonstrate for the first time a complete small protein characterization with the projection-decomposition approach, including full assignments as well as determination of the 3D fold. In TOCSY- and NOESY-type 4D experiments, pairing of signals from hydrogens and from their...
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11-19-2016 08:35 PM
New technique to analyze conformations of complex molecular machines - Phys.Org
http://www.bionmr.com//t2.gstatic.com/images?q=tbn:ANd9GcRo1TkQ4r1fMpUxa3wD5SK2d2V8T_oG2l78aZVQEcL_XDMCV_pyy0Biarzf0xAik6wok_0CyH8
Phys.Org
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New technique to analyze conformations of complex molecular machines
Phys.Org
"Structural techniques like X-ray crystallography and nuclear magnetic resonance have worked quite well to help us understand how smaller proteins function," Onuchic said. X-rays only take snapshots of constantly moving proteins, he said, "but ...
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New technique to analyze conformations of complex molecular machines...
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03-14-2014 07:34 PM
Proton NMR - How To Analyze The Peaks Of H-NMR Spectroscopy
Proton NMR - How To Analyze The Peaks Of H-NMR Spectroscopy
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Proton NMR - How To Analyze The Peaks Of H-NMR Spectroscopy
Download My ebook: "10 Secrets To Acing Organic Chemistry" forms.aweber.com leah4sci.com Presents: H-NMR How To Analyze Peaks This video takes you through the four key aspects you need to understand when interpreting the peaks of an H-NMR graph - Number and type of unique hydrogen - splitting of peaks - hydrogen neighbors - chemical shifts Future videos will take you through specific NMR problems and solving techniques...
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02-27-2013 06:45 AM
[NMR paper] An advantage for use of isotope labeling and NMR chemical shifts to analyze the struc
An advantage for use of isotope labeling and NMR chemical shifts to analyze the structure of four homologous IgG-binding domains of staphylococcal protein A.
Related Articles An advantage for use of isotope labeling and NMR chemical shifts to analyze the structure of four homologous IgG-binding domains of staphylococcal protein A.
J Biochem Biophys Methods. 2000 Jan 3;42(1-2):35-47
Authors: Kikuchi J, Asakura T, Hasuda K, Ito T, Ohwaku K, Araki H, Williamson MP
Because of the complexity arising from the large molecular size and the amino acid...
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11-18-2010 09:15 PM
[Question from NMRWiki Q&A forum] How to process and analyze serial 1D data nmrPipe?
How to process and analyze serial 1D data nmrPipe?
Hi all,I am very new to nmrPipe. I want to achieve the following steps. It would be very kind if somebody helps.
I have a 2D bruker data (t2= 4k points, t1= 32 points) and I want to do Fourier transform in the F2 dimension,phase etc. (2) and then extract the 32 spectra as 1Ds and also convert these 32 spectra in ascii format.(3) fit the peaks with a gaussian or Lorentzian function.(4) integrate the peak and save the integrals and time points as an ascii file.
I only managed till the 1st step.Please help..!!
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News from other NMR forums
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10-28-2010 09:14 PM
[NMR paper] Use of 1H NMR spectroscopy and computer simulations To analyze histidine pKa changes
Use of 1H NMR spectroscopy and computer simulations To analyze histidine pKa changes in a protein tyrosine phosphatase: experimental and theoretical determination of electrostatic properties in a small protein.
http://www.ncbi.nlm.nih.gov/corehtml/query/egifs/http:--pubs.acs.org-images-acspubs.jpg Related Articles Use of 1H NMR spectroscopy and computer simulations To analyze histidine pKa changes in a protein tyrosine phosphatase: experimental and theoretical determination of electrostatic properties in a small protein.
Biochemistry. 1997 Sep 30;36(39):11984-94
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XPS and NMR analyze the combined forms of Pb in Cladophora rupestris subcells and its detoxification
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