Related ArticlesWhen Less Is More: Combining Site-Specific Isotope Labeling and NMR Unravels Structural Details of Huntingtin Repeats.
Structure. 2020 Jul 07;28(7):730-732
Authors: Delhommel F, Sattler M
Abstract
In this issue of Structure, Urbanek et*al. (2020a) combine site-specific isotope labeling and NMR spectroscopy to investigate opposing effects of flanking regions onto the conformation of the poly-Q region in Huntingtin. Poly-Q interactions with preceding residues promote an ?-helical conformation while a following proline-rich region favors extended conformations.
[NMR paper] Site-Specific Isotope-Labeling of Inosine Phosphoramidites and NMR Analysis of an Inosine-Containing RNA Duplex.
Site-Specific Isotope-Labeling of Inosine Phosphoramidites and NMR Analysis of an Inosine-Containing RNA Duplex.
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Chemistry. 2016 Oct 17;22(43):15350-15359
Authors: Dallmann A, Beribisky AV, Gnerlich F, Rübbelke M, Schiesser S, Carell T, Sattler M
Abstract
Structural features and internal dynamics of inosine-containing RNAs are poorly understood. NMR studies of such RNAs require 13 C,15 N-labeling,...
[NMR paper] Methyl-Specific Isotope Labeling Strategies for NMR Studies of Membrane Proteins.
Methyl-Specific Isotope Labeling Strategies for NMR Studies of Membrane Proteins.
Related Articles Methyl-Specific Isotope Labeling Strategies for NMR Studies of Membrane Proteins.
Methods Mol Biol. 2017;1635:109-123
Authors: Kurauskas V, Schanda P, Sounier R
Abstract
Methyl groups are very useful probes of structure, dynamics, and interactions in protein NMR spectroscopy. In particular, methyl-directed experiments provide high sensitivity even in very large proteins, such as membrane proteins in a membrane-mimicking...
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Combining DNP NMR with segmental and specific labeling to study a yeast prion protein strain that is not parallel in-register #DNPNMR
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Combining DNP NMR with segmental and specific labeling to study a yeast prion protein strain that is not parallel in-register #DNPNMR
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Frederick, K.K., et al., Combining DNP NMR with segmental and specific labeling to study a yeast prion protein strain that is not parallel in-register. Proc. Nat. Aca. Sci. USA, 2017. 114(14): p. 3642-3647.
http://www.pnas.org/content/114/14/3642.abstract
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04-24-2017 02:56 PM
Combining DNP NMR with segmental and specific labeling to study a yeast prion protein strain that is not parallel in-register [Biophysics and Computational Biology]
Combining DNP NMR with segmental and specific labeling to study a yeast prion protein strain that is not parallel in-register
Kendra K. Frederick, Vladimir K. Michaelis, Marc A. Caporini, Loren B. Andreas, Galia T. Debelouchina, Robert G. Griffin, Susan Lindquist...
Date: 2017-04-04
The yeast prion protein Sup35NM is a self-propagating amyloid. Despite intense study, there is no consensus on the organization of monomers within Sup35NM fibrils. Some studies point to a ?-helical arrangement, whereas others suggest a parallel in-register organization. Intermolecular contacts are often...
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04-04-2017 10:41 PM
[NMR paper] Combining DNP NMR with segmental and specific labeling to study a yeast prion protein strain that is not parallel in-register.
Combining DNP NMR with segmental and specific labeling to study a yeast prion protein strain that is not parallel in-register.
Related Articles Combining DNP NMR with segmental and specific labeling to study a yeast prion protein strain that is not parallel in-register.
Proc Natl Acad Sci U S A. 2017 Mar 22;:
Authors: Frederick KK, Michaelis VK, Caporini MA, Andreas LB, Debelouchina GT, Griffin RG, Lindquist S
Abstract
The yeast prion protein Sup35NM is a self-propagating amyloid. Despite intense study, there is no consensus on...
[NMR paper] Combining inducible protein overexpression with NMR-grade triple isotope labeling in the cyanobacterium Anabaena sp. PCC 7120.
Combining inducible protein overexpression with NMR-grade triple isotope labeling in the cyanobacterium Anabaena sp. PCC 7120.
Related Articles Combining inducible protein overexpression with NMR-grade triple isotope labeling in the cyanobacterium Anabaena sp. PCC 7120.
Biotechniques. 2005 Sep;39(3):405-11
Authors: Desplancq D, Bernard C, Sibler AP, Kieffer B, Miguet L, Potier N, Van Dorsselaer A, Weiss E
The difficulty and expense of preparing protein samples highly enriched in stable isotopes is a bottleneck for structural studies by nuclear...
When Less Is More: Combining Site-Specific Isotope Labeling and NMR Unravels Structural Details of Huntingtin Repeats.
Linear Mode
