[NMR paper] In vivo and in vitro metabolism of a novel ?2-adrenoceptor agonist, trantinterol: metabolites isolation and identification by LC-MS/MS and NMR.
Related ArticlesIn vivo and in vitro metabolism of a novel ?2-adrenoceptor agonist, trantinterol: metabolites isolation and identification by LC-MS/MS and NMR.
Anal Bioanal Chem. 2013 Mar;405(8):2619-34
Authors: Li K, Qin F, Jing L, Li F, Guo X
Abstract
Trantinterol is a novel ?(2)-adrenoceptor agonist used for the treatment of asthma. The aim of this study is to identify the metabolites of trantinterol using liquid chromatography tandem mass spectrometry (LC-MS/MS), to isolate the main metabolites, and confirm their structures by nuclear magnetic resonance (NMR). Urine, feces, bile, and blood samples of rats were obtained and analyzed. Reference standards of six metabolites were achieved with the combination of chemical synthesis, microbial transformation, and the model systems of rats. Moreover, in order to investigate the phase I metabolism of trantinterol in humans and to study the species differences between rats and humans, incubations with liver microsomes were performed. The biotransformation by a microbial model Cunninghamella blakesleana AS 3.970 was also studied. A total of 18 metabolites were identified in vivo and in vitro together, 13 of which were newly detected. Three phase I metabolites were detected in vivo and in vitro as well as in the microbial model, including the arylhydroxylamine (M1), the tert-butyl hydroxylated trantinterol (M2) and the 1-carbonyltrantinterol (M3). Another important pathway in rats is glutathione conjugation and further catabolism and oxidation to form consecutive derivatives (M4 through M10). Other metabolites include glucuronide, glucoside, and sulfate conjugates. The results of in vitro experiments indicate no species difference exists among rats, humans, and C. blakesleana AS 3.970 on the phase I metabolism of trantinterol. Our study provided the most comprehensive picture for trantinterol in vivo and in vitro metabolism to this day, and may predict its metabolism in humans.
[NMR paper] (1)H NMR spectroscopic studies on the characterization of renal cell lines and identification of novel potential markers of in vitro nephrotoxicity.
(1)H NMR spectroscopic studies on the characterization of renal cell lines and identification of novel potential markers of in vitro nephrotoxicity.
Related Articles (1)H NMR spectroscopic studies on the characterization of renal cell lines and identification of novel potential markers of in vitro nephrotoxicity.
Biomarkers. 1996;1(1):35-43
Authors: Anthony ML, McDowell PC, Gray TJ, Blackmore M, Nicholson JK
Abstract
Abstract Cell cultures are increasingly used in the evaluation of chemically-induced nephrotoxicity. The utili of renal cell...
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[NMR paper] NMR identification of endogenous metabolites interacting with fatted and non-fatted human serum albumin in blood plasma: Fatty acids influence the HSA-metabolite interaction.
NMR identification of endogenous metabolites interacting with fatted and non-fatted human serum albumin in blood plasma: Fatty acids influence the HSA-metabolite interaction.
http://www.ncbi.nlm.nih.gov/corehtml/query/egifs/http:--linkinghub.elsevier.com-ihub-images-PubMedLink.gif Related Articles NMR identification of endogenous metabolites interacting with fatted and non-fatted human serum albumin in blood plasma: Fatty acids influence the HSA-metabolite interaction.
J Magn Reson. 2013 Jan 8;228C:81-94
Authors: Jupin M, Michiels PJ, Girard FC, Spraul M,...
In vivo oxygen-17 NMR for imaging brain oxygen metabolism at high field
In vivo oxygen-17 NMR for imaging brain oxygen metabolism at high field
Publication year: 2011
Source:Progress in Nuclear Magnetic Resonance Spectroscopy, Volume 59, Issue 4</br>
Xiao-Hong Zhu, Wei Chen</br>
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In vivo oxygen-17 NMR for imaging brain oxygen metabolism at high field
In vivo oxygen-17 NMR for imaging brain oxygen metabolism at high field
Publication year: 2011
Source: Progress in Nuclear Magnetic Resonance Spectroscopy, In Press, Accepted Manuscript, Available online 23 April 2011</br>
Xiao-Hong, Zhu , Wei, Chen</br>
*Highlights:*? This article reviews the developments of in vivo 17O NMR imaging in brain research. ? In vivo 17O NMR imaging has improved significantly at high/ultrahigh field. ? In vivo 17O NMR can noninvasively image brain oxygen metabolism and perfusion. ? In vivo 17O NMR is useful for mapping the functional change in oxygen...
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[NMR paper] In vivo 13C and 15N NMR studies of methylamine metabolism in Pseudomonas species MA.
In vivo 13C and 15N NMR studies of methylamine metabolism in Pseudomonas species MA.
Related Articles In vivo 13C and 15N NMR studies of methylamine metabolism in Pseudomonas species MA.
J Biol Chem. 1991 Jun 25;266(18):11705-13
Authors: Jones JG, Bellion E
Pseudomonas species MA was grown with methylamine as a sole source of carbon and nitrogen enabling the total flow of carbon and nitrogen into this organism to be simultaneously monitored in vivo using 13C and 15N NMR. Methylamine was rapidly and extensively incorporated into the methyl...
In vivo and in vitro metabolism of a novel ?2-adrenoceptor agonist, trantinterol: metabolites isolation and identification by LC-MS/MS and NMR.
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