[NMR paper] In vivo spectroscopy and NMR metabolite fingerprinting approaches to connect the dynamics of photosynthetic and metabolic phenotypes in resurrection plant Haberlea rhodopensis during desiccation and recovery.
In vivo spectroscopy and NMR metabolite fingerprinting approaches to connect the dynamics of photosynthetic and metabolic phenotypes in resurrection plant Haberlea rhodopensis during desiccation and recovery.
Related ArticlesIn vivo spectroscopy and NMR metabolite fingerprinting approaches to connect the dynamics of photosynthetic and metabolic phenotypes in resurrection plant Haberlea rhodopensis during desiccation and recovery.
Front Plant Sci. 2015;6:564
Authors: Mladenov P, Finazzi G, Bligny R, Moyankova D, Zasheva D, Boisson AM, Brugière S, Krasteva V, Alipieva K, Simova S, Tchorbadjieva M, Goltsev V, Ferro M, Rolland N, Djilianov D
Abstract
The resurrection plant Haberlea rhodopensis was used to study dynamics of drought response of photosynthetic machinery parallel with changes in primary metabolism. A relation between leaf water content and photosynthetic performance was established, enabling us to perform a non-destructive evaluation of the plant water status during stress. Spectroscopic analysis of photosynthesis indicated that, at variance with linear electron flow (LEF) involving photosystem (PS) I and II, cyclic electron flow around PSI remains active till almost full dry state at the expense of the LEF, due to the changed protein organization of photosynthetic apparatus. We suggest that, this activity could have a photoprotective role and prevent a complete drop in adenosine triphosphate (ATP), in the absence of LEF, to fuel specific energy-dependent processes necessary for the survival of the plant, during the late states of desiccation. The NMR fingerprint shows the significant metabolic changes in several pathways. Due to the declining of LEF accompanied by biosynthetic reactions during desiccation, a reduction of the ATP pool during drought was observed, which was fully and quickly recovered after plants rehydration. We found a decline of valine accompanied by lipid degradation during stress, likely to provide alternative carbon sources for sucrose accumulation at late stages of desiccation. This accumulation, as well as the increased levels of glycerophosphodiesters during drought stress could provide osmoprotection to the cells.
Hyperpolarization without persistent radicals for in vivo real-time metabolic imaging
From The DNP-NMR Blog:
Hyperpolarization without persistent radicals for in vivo real-time metabolic imaging
Eichhorn, T.R., et al., Hyperpolarization without persistent radicals for in vivo real-time metabolic imaging. Proc. Nat. Aca. Sci. USA, 2013. 110(45): p. 18064-18069.
http://www.pnas.org/content/110/45/18064.abstract
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07-28-2014 08:52 PM
Hyperpolarized 13C dehydroascorbate as an endogenous redox sensor for in vivo metabolic imaging
From The DNP-NMR Blog:
Hyperpolarized 13C dehydroascorbate as an endogenous redox sensor for in vivo metabolic imaging
Keshari, K.R., et al., Hyperpolarized 13C dehydroascorbate as an endogenous redox sensor for in vivo metabolic imaging. Proc. Nat. Aca. Sci. USA, 2011. 108(46): p. 18606-18611.
http://www.pnas.org/content/108/46/18606.abstract
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07-23-2014 11:25 PM
Journal Highlight: 1H NMR metabolite fingerprinting as a new tool for body fluid identification in forensic science
Journal Highlight: 1H NMR metabolite fingerprinting as a new tool for body fluid identification in forensic science
http://www.spectroscopynow.com/common/images/thumbnails/140de1a0264.jpg1H NMR spectroscopy coupled with mathematical strategies has been proposed for the first time as a fast and non-destructive tool for body fluid trace identification in forensic science.
Read the rest at Spectroscopynow.com
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General
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09-02-2013 10:45 PM
1H NMR-based metabolic fingerprinting of urine metabolites after consumption of lingonberries (Vaccinium vitis-idaea) with a high-fat meal
1H NMR-based metabolic fingerprinting of urine metabolites after consumption of lingonberries (Vaccinium vitis-idaea) with a high-fat meal
15 May–1 June 2013
Publication year: 2013
Source:Food Chemistry, Volume 138, Issues 2–3</br>
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The use of NMR metabolomics in clinical trials is growing; however, reports of postprandial experiments in humans are scarce. The present study investigated whether consumption of lingonberries as a supplement to an oil-rich meal modifies the postprandial fingerprints of human urine. Urine samples were analysed by 1H NMR, and untargeted...
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Journal club
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02-03-2013 10:05 AM
Mutations in the Saccharomyces cerevisiae succinate dehydrogenase result in distinct metabolic phenotypes revealed through (1)H NMR-based metabolic footprinting.
Mutations in the Saccharomyces cerevisiae succinate dehydrogenase result in distinct metabolic phenotypes revealed through (1)H NMR-based metabolic footprinting.
Mutations in the Saccharomyces cerevisiae succinate dehydrogenase result in distinct metabolic phenotypes revealed through (1)H NMR-based metabolic footprinting.
J Proteome Res. 2010 Dec 3;9(12):6729-39
Authors: Szeto SS, Reinke SN, Sykes BD, Lemire BD
Metabolomics is a powerful method of examining the intricate connections between mutations, metabolism, and disease. Metabolic...
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Journal club
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05-25-2011 07:01 PM
[NMR paper] In vivo 13C NMR metabolite profiling: potential for understanding and assessing conifer seed quality.
In vivo 13C NMR metabolite profiling: potential for understanding and assessing conifer seed quality.
Related Articles In vivo 13C NMR metabolite profiling: potential for understanding and assessing conifer seed quality.
J Exp Bot. 2005 Aug;56(418):2253-65
Authors: Terskikh VV, Feurtado JA, Borchardt S, Giblin M, Abrams SR, Kermode AR
High-resolution 13C MAS NMR spectroscopy was used to profile a range of primary and secondary metabolites in vivo in intact whole seeds of eight different conifer species native to North America, including six of...
In vivo spectroscopy and NMR metabolite fingerprinting approaches to connect the dynamics of photosynthetic and metabolic phenotypes in resurrection plant Haberlea rhodopensis during desiccation and recovery.
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