[NMR paper] In vivo Proton NMR spectroscopy of genetic mouse models BALB/cJ and C57BL/6By: variation in hippocampal glutamate level and the metabotropic glutamate receptor, subtype 7 (Grm7) gene.
In vivo Proton NMR spectroscopy of genetic mouse models BALB/cJ and C57BL/6By: variation in hippocampal glutamate level and the metabotropic glutamate receptor, subtype 7 (Grm7) gene.
Related ArticlesIn vivo Proton NMR spectroscopy of genetic mouse models BALB/cJ and C57BL/6By: variation in hippocampal glutamate level and the metabotropic glutamate receptor, subtype 7 (Grm7) gene.
J Mol Neurosci. 2014 May;53(1):135-41
Authors: Guilfoyle DN, Gerum S, Vadasz C
Abstract
Glutamatergic neurotransmission in the brain is modulated by metabotropic glutamate receptors (mGluR). In recent studies, we identified a cis-regulated variant of a gene (Grm7) which codes for mGluR subtype 7 (mGluR7), a presynaptic inhibitory receptor. The genetic variant derived from the BALB/cJ mouse strain (Grm7 (BALB/cJ)) codes for higher abundance of mGluR7 mRNA in the hippocampus than the C57BL/6By strain-derived variant (Grm7 (C57BL/6By)). Here, we used localized in vivo (1)H NMR spectroscopy to test the hypothesis that Grm7 (BALB/cJ) is also associated with lower glutamate concentration in the same brain region. All data were obtained on a 7.0 T Agilent (Santa Clara, CA, USA) 40-cm bore system using experimentally naive adult male inbred C57BL/6By, BALB/cJ, and congenic mice (B6By.C.6.132.54) constructed in our laboratory carrying Grm7 (BALB/cJ) on C57BL/6By genetic background. The voxel of interest size was 6 ?L (1 × 2 × 3 mm(3)) placed in the hippocampal CA1 region. The results showed that the hippocampal level of glutamate in the congenic mouse strain was significantly lower than that in the background C57BL/6By strain which carried the Grm7 (C57BL/6By) allele. Because the two inbred strains are genetically highly similar except at the region of the Grm7 gene, the results raise the possibility that allelic variation at the Grm7 locus contributes to the strain differences in both hippocampal mRNA abundance and glutamate level which may modulate complex behavioral traits, such as learning and memory, addiction, epilepsy, and mood disorders.
Journal Highlight: NMR structures of fusion peptide from influenza hemagglutinin H3 subtype and its mutants
Journal Highlight: NMR structures of fusion peptide from influenza hemagglutinin H3 subtype and its mutants
http://www.spectroscopynow.com/common/images/thumbnails/14513e996b9.jpgThe structures of the influenza hemagglutinin H3-HAfp23 peptide and its mutants, G1S and G1V, in dodecylphosphatidyl choline micelles were studied by heteronuclear NMR spectroscopy to study the role of its amino acids in the fusion process.
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nmrlearner
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03-31-2014 10:16 AM
[NMR paper] NMR spectroscopy reveals unexpected structural variation at the protein-protein interface in MHC class I molecules.
NMR spectroscopy reveals unexpected structural variation at the protein-protein interface in MHC class I molecules.
NMR spectroscopy reveals unexpected structural variation at the protein-protein interface in MHC class I molecules.
J Biomol NMR. 2013 Sep 5;
Authors: Beerbaum M, Ballaschk M, Erdmann N, Schnick C, Diehl A, Uchanska-Ziegler B, Ziegler A, Schmieder P
Abstract
?2-Microglobulin (?2m) is a small, monomorphic protein non-covalently bound to the heavy chain (HC) in polymorphic major histocompatibility complex (MHC) class I...
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09-06-2013 06:52 PM
A metabolomic comparison of mouse models of the Neuronal Ceroid Lipofuscinoses
A metabolomic comparison of mouse models of the Neuronal Ceroid Lipofuscinoses
Abstract The Neuronal Ceroid Lipofuscinoses (NCL) are a group of fatal inherited neurodegenerative diseases in humans distinguished by a common clinical pathology, characterized by the accumulation of storage body material in cells and gross brain atrophy. In this study, metabolic changes in three NCL mouse models were examined looking for pathways correlated with neurodegeneration. Two mouse models; motor neuron degeneration (mnd) mouse and a variant model of late infantile NCL, termed the neuronal ceroid...
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04-05-2011 10:53 PM
Role of aminotransferases in glutamate metabolism of human erythrocytes
Role of aminotransferases in glutamate metabolism of human erythrocytes
Abstract Human erythrocytes require a continual supply of glutamate to support glutathione synthesis, but are unable to transport this amino acid across their cell membrane. Consequently, erythrocytes rely on de novo glutamate biosynthesis from α-ketoglutarate and glutamine to maintain intracellular levels of glutamate. Erythrocytic glutamate biosynthesis is catalyzed by three enzymes, alanine aminotransferase (ALT), aspartate aminotransferase (AST), and glutamine aminohydrolase (GA). Although the presence of these...
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03-09-2011 04:19 AM
[NMR paper] Measurement of side-chain carboxyl pK(a) values of glutamate and aspartate residues i
Measurement of side-chain carboxyl pK(a) values of glutamate and aspartate residues in an unfolded protein by multinuclear NMR spectroscopy.
Related Articles Measurement of side-chain carboxyl pK(a) values of glutamate and aspartate residues in an unfolded protein by multinuclear NMR spectroscopy.
J Am Chem Soc. 2002 May 22;124(20):5714-7
Authors: Tollinger M, Forman-Kay JD, Kay LE
A triple-resonance NMR pulse scheme is presented for measuring aspartic and glutamic acid side-chain pK(a) values in unfolded protein states where chemical shift...
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11-24-2010 08:49 PM
[NMR paper] Determination of NADH-dependent glutamate synthase (GOGAT) in Spodoptera frugiperda (
Determination of NADH-dependent glutamate synthase (GOGAT) in Spodoptera frugiperda (Sf9) insect cells by a selective 1H/15N NMR in vitro assay.
Related Articles Determination of NADH-dependent glutamate synthase (GOGAT) in Spodoptera frugiperda (Sf9) insect cells by a selective 1H/15N NMR in vitro assay.
J Biotechnol. 2000 Apr 14;79(1):87-97
Authors: Doverskog M, Jacobsson U, Chapman BE, Kuchel PW, Häggström L
This is the second of two papers . where the general goal has been to determine and characterise the glutamine metabolism in Sf9...
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11-18-2010 09:15 PM
[NMR paper] In vitro proton and phosphorus NMR spectroscopic analysis of murine (C57Bl/6J) brain
In vitro proton and phosphorus NMR spectroscopic analysis of murine (C57Bl/6J) brain development.
Related Articles In vitro proton and phosphorus NMR spectroscopic analysis of murine (C57Bl/6J) brain development.
NMR Biomed. 1999 Nov;12(7):463-70
Authors: Yao FS, Caserta MT, Wyrwicz AM
We report for the first time in vitro proton and phosphorus NMR spectroscopic analyses of murine brain development from fetal to adult stages. Chloroform-methanol extracts from C57B16/J mouse brain, at ages ranging from 15 days in utero (F15) to adult, permitted...
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11-18-2010 08:31 PM
[NMR paper] Proton NMR sequence-specific assignments and secondary structure of a receptor bindin
Proton NMR sequence-specific assignments and secondary structure of a receptor binding domain of mouse gamma-interferon.
Related Articles Proton NMR sequence-specific assignments and secondary structure of a receptor binding domain of mouse gamma-interferon.
Biochemistry. 1993 Jun 1;32(21):5650-5
Authors: Sakai TT, Jablonsky MJ, DeMuth PA, Krishna NR, Jarpe MA, Johnson HM
Previous studies using synthetic peptides and monoclonal antibodies have implicated the N-terminal 39-residue segment as a receptor binding region of mouse gamma-interferon...
In vivo Proton NMR spectroscopy of genetic mouse models BALB/cJ and C57BL/6By: variation in hippocampal glutamate level and the metabotropic glutamate receptor, subtype 7 (Grm7) gene.
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