Related ArticlesIn vitro proton and phosphorus NMR spectroscopic analysis of murine (C57Bl/6J) brain development.
NMR Biomed. 1999 Nov;12(7):463-70
Authors: Yao FS, Caserta MT, Wyrwicz AM
We report for the first time in vitro proton and phosphorus NMR spectroscopic analyses of murine brain development from fetal to adult stages. Chloroform-methanol extracts from C57B16/J mouse brain, at ages ranging from 15 days in utero (F15) to adult, permitted the simultaneous investigation of both cytosolic and membrane phospholipid compartments. The protein content of murine brain was determined and used for quantitation of individual metabolite levels. Proton NMR spectroscopy revealed that NAA, considered a neuronal marker, is undetectable at F15. Glutamate, GABA and creatine, however, are present at this time. All four compounds reach maximum levels at 21 days postnatal (P21). Choline and alanine levels are at their peak in fetal brain and progressively fall as the brain develops. Phosphorus NMR spectroscopy shows that phosphatidylcholine, phosphatidylinositol, sphingomyelin, and phosphatidylserine increase steadily from F15 to P21.
[Question from NMRWiki Q&A forum] Bruker Tpospin for brain spectroscopy analysis
Bruker Tpospin for brain spectroscopy analysis
Hi I am using Bruker Tpospin for brain spectroscopy analysis. I am new to this software. My question is how do I make spectral lines dotted or thicker instead of colors. Any hint is highly appreciated. ThanksSurya
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[NMR paper] NMR analysis of in vitro-synthesized proteins without purification: a high-throughput
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FEBS Lett. 2002 Jul 31;524(1-3):159-62
Authors: Guignard L, Ozawa K, Pursglove SE, Otting G, Dixon NE
A cell-free protein expression system was established that provides protein samples of adequate concentration and purity for direct NMR analysis. The Escherichia coli peptidyl-prolyl cis-trans isomerase PpiB was expressed in this system...
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11-24-2010 08:58 PM
[NMR paper] In vitro NMR proton T1 measurements in peritoneal ascites.
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http://www.ncbi.nlm.nih.gov/corehtml/query/egifs/http:--ej.iop.org-icons-external-pubmed-iop-ej.gif Related Articles In vitro NMR proton T1 measurements in peritoneal ascites.
Phys Med Biol. 1996 Mar;41(3):539-49
Authors: Yilmaz A, Tez M, Göral V, Boylu S, Kaplan A, Kavak G
The proton spin-lattice relaxation rate (1/T1) in malignant and non-malignant ascites was measured with an FT NMR spectrometer operating at 60 MHz. The mean relaxation rate in non-malignant ascites was...
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08-22-2010 02:27 PM
[NMR paper] Proton NMR studies on ischemic rat brain tissue.
Proton NMR studies on ischemic rat brain tissue.
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Magn Reson Med. 1992 May;25(1):78-84
Authors: Iwama T, Yamada H, Andoh T, Sakai N, Era S, Sogami M, Kuwata K, Watari H
The spin-lattice relaxation time (T1) of water protons and the cross-relaxation time (TIS) between irradiated protein protons and observed water protons were measured in order to study water-macromolecular interactions in ischemic rat brain tissues. Tissues were obtained by bilateral common carotid artery...
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[NMR paper] Cephaloridine-induced nephrotoxicity in the Fischer 344 rat: proton NMR spectroscopic
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Arch Toxicol. 1992;66(8):525-37
Authors: Anthony ML, Gartland KP, Beddell CR, Lindon JC, Nicholson JK
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[NMR paper] Analysis of conventional and in vitro generated mutants of nmr, the negatively acting
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Mol Gen Genet. 1990 Jul;222(2-3):233-40
Authors: Jarai G, Marzluf GA
The nmr gene is the major negative regulatory gene in the nitrogen control circuit of Neurospora crassa, which, together with positive regulatory genes, governs the expression of multiple unlinked...
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[NMR paper] NMR analysis of low-density lipoprotein oxidatively-modified in vitro.
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Free Radic Res Commun. 1990;8(3):175-83
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Human plasma low density lipoprotein has been oxidized at different stages in vitro and analysed by 1H, 13C, and 31P NMR spectroscopy and by biochemical methods. Information was obtained on: a) structure mobilities of lipids and on lipid-protein interactions; b) conjugated and oxo-dienes; c)...
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[NMR paper] An in vitro kinetic assay of ATPase by phosphorus-31 NMR.
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Kurume Med J. 1990;37(3):153-7
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The ATPase activity of RecA protein was examined by monitoring the changes of NMR phosphorus signals of ATP, ADP and inorganic phosphate. The areas of phosphorus-31 NMR peaks from inorganic phosphate and ADP, which increased with time, and the signals from ATP, which decreased with time, were fitted by a linear least square method to obtain the initial...
In vitro proton and phosphorus NMR spectroscopic analysis of murine (C57Bl/6J) brain
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