Related ArticlesIn vitro NMR proton T1 measurements in peritoneal ascites.
Phys Med Biol. 1996 Mar;41(3):539-49
Authors: Yilmaz A, Tez M, Göral V, Boylu S, Kaplan A, Kavak G
The proton spin-lattice relaxation rate (1/T1) in malignant and non-malignant ascites was measured with an FT NMR spectrometer operating at 60 MHz. The mean relaxation rate in non-malignant ascites was significantly smaller than that of malignant ascites. However, the 1/T1 of malignant ascites overlaps with that of non-malignant ascites over all concentrations of total protein (TP) in samples. The 1/T1 in non-malignant ascites correlates strongly with TP, whilst the 1/T1 in malignant ascites shows only a moderate correlation. T1 measurements before and after addition of ascorbic acid (reductant) suggest that there is a small paramagnetic contribution of ions to the 1/T1 in malignant ascites. The least-squares fitting of 1/T1 versus TP for non-malignant data gives a linear relationship, and suggests that the T1 mechanism in non-malignant ascites is caused by a fast chemical exchange of water molecules between protein-bound water and free water.
Conformational analysis by quantitative NOE measurements of the β-proton pairs across individual disulfide bonds in proteins
Conformational analysis by quantitative NOE measurements of the β-proton pairs across individual disulfide bonds in proteins
Abstract NOEs between the β-protons of cysteine residues across disulfide bonds in proteins provide direct information on the connectivities and conformations of these important cross-links, which are otherwise difficult to investigate. With conventional -proteins, however, fast spin diffusion processes mediated by strong dipolar interactions between geminal β-protons prohibit the quantitative measurements and thus the analyses of long-range NOEs across...
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[NMR paper] Changes in orcine muscle water characteristics during growth--an in vitro low-field N
Changes in orcine muscle water characteristics during growth--an in vitro low-field NMR relaxation study.
Related Articles Changes in orcine muscle water characteristics during growth--an in vitro low-field NMR relaxation study.
J Magn Reson. 2002 Aug;157(2):267-76
Authors: Bertram HC, Rasmussen M, Busk H, Oksbjerg N, Karlsson AH, Andersen HJ
This study investigates the effects of developmental stage and muscle type on the mobility and distribution of water within skeletal muscles, using low-field (1)H-NMR transverse relaxation measurements in...
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[NMR paper] NMR analysis of in vitro-synthesized proteins without purification: a high-throughput
NMR analysis of in vitro-synthesized proteins without purification: a high-throughput approach.
Related Articles NMR analysis of in vitro-synthesized proteins without purification: a high-throughput approach.
FEBS Lett. 2002 Jul 31;524(1-3):159-62
Authors: Guignard L, Ozawa K, Pursglove SE, Otting G, Dixon NE
A cell-free protein expression system was established that provides protein samples of adequate concentration and purity for direct NMR analysis. The Escherichia coli peptidyl-prolyl cis-trans isomerase PpiB was expressed in this system...
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[NMR paper] In vitro proton and phosphorus NMR spectroscopic analysis of murine (C57Bl/6J) brain
In vitro proton and phosphorus NMR spectroscopic analysis of murine (C57Bl/6J) brain development.
Related Articles In vitro proton and phosphorus NMR spectroscopic analysis of murine (C57Bl/6J) brain development.
NMR Biomed. 1999 Nov;12(7):463-70
Authors: Yao FS, Caserta MT, Wyrwicz AM
We report for the first time in vitro proton and phosphorus NMR spectroscopic analyses of murine brain development from fetal to adult stages. Chloroform-methanol extracts from C57B16/J mouse brain, at ages ranging from 15 days in utero (F15) to adult, permitted...
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[NMR paper] Specific in vitro labeling of cells with a fluorine-19 probe encapsulated in antibody
Specific in vitro labeling of cells with a fluorine-19 probe encapsulated in antibody-targeted liposomes: a F-19 NMR spectroscopy study.
Related Articles Specific in vitro labeling of cells with a fluorine-19 probe encapsulated in antibody-targeted liposomes: a F-19 NMR spectroscopy study.
Magn Reson Med. 1993 Feb;29(2):252-5
Authors: Vion-Dury J, Machy P, Confort-Gouny S, Leserman L, Cozzone PJ
Liposomes containing dexamethasone phosphate (DMp) were covalently coupled to protein A and then incubated with murine L929 fibroblast and RDM4...
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[NMR paper] Analysis of conventional and in vitro generated mutants of nmr, the negatively acting
Analysis of conventional and in vitro generated mutants of nmr, the negatively acting nitrogen regulatory gene of Neurospora crassa.
Related Articles Analysis of conventional and in vitro generated mutants of nmr, the negatively acting nitrogen regulatory gene of Neurospora crassa.
Mol Gen Genet. 1990 Jul;222(2-3):233-40
Authors: Jarai G, Marzluf GA
The nmr gene is the major negative regulatory gene in the nitrogen control circuit of Neurospora crassa, which, together with positive regulatory genes, governs the expression of multiple unlinked...
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[NMR paper] NMR analysis of low-density lipoprotein oxidatively-modified in vitro.
NMR analysis of low-density lipoprotein oxidatively-modified in vitro.
Related Articles NMR analysis of low-density lipoprotein oxidatively-modified in vitro.
Free Radic Res Commun. 1990;8(3):175-83
Authors: Barenghi L, Bradamante S, Giudici GA, Vergani C
Human plasma low density lipoprotein has been oxidized at different stages in vitro and analysed by 1H, 13C, and 31P NMR spectroscopy and by biochemical methods. Information was obtained on: a) structure mobilities of lipids and on lipid-protein interactions; b) conjugated and oxo-dienes; c)...
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[NMR paper] An in vitro kinetic assay of ATPase by phosphorus-31 NMR.
An in vitro kinetic assay of ATPase by phosphorus-31 NMR.
Related Articles An in vitro kinetic assay of ATPase by phosphorus-31 NMR.
Kurume Med J. 1990;37(3):153-7
Authors: Chinami M, Shingu M
The ATPase activity of RecA protein was examined by monitoring the changes of NMR phosphorus signals of ATP, ADP and inorganic phosphate. The areas of phosphorus-31 NMR peaks from inorganic phosphate and ADP, which increased with time, and the signals from ATP, which decreased with time, were fitted by a linear least square method to obtain the initial...
In vitro NMR proton T1 measurements in peritoneal ascites.
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