In-cell NMR spectroscopy is a powerful tool to investigate protein behavior in physiologically relevant environments. Although proven valuable for disordered proteins, we show that in commonly used 1 H- 15 N HSQC spectra of globular proteins, interactions with cellular components often broaden resonances beyond detection. This contrasts 19 F spectra in mammalian cells, in which signals are readily observed. Using several proteins, we demonstrate that surface charges and interaction with cellular...
[NMR paper] In-Cell NMR of Intrinsically Disordered Proteins in Mammalian Cells.
In-Cell NMR of Intrinsically Disordered Proteins in Mammalian Cells.
Related Articles In-Cell NMR of Intrinsically Disordered Proteins in Mammalian Cells.
Methods Mol Biol. 2020;2141:873-893
Authors: Gerez JA, Prymaczok NC, Riek R
Abstract
In-cell NMR enables structural insights at atomic resolution of proteins in their natural environment. To date, very few methods have been developed to study proteins by in-cell NMR in mammalian systems. Here we describe a detailed protocol to conduct in-cell NMR on the intrinsically...
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[NMR paper] Recording In-Cell NMR-Spectra in Living Mammalian Cells.
Recording In-Cell NMR-Spectra in Living Mammalian Cells.
Related Articles Recording In-Cell NMR-Spectra in Living Mammalian Cells.
Methods Mol Biol. 2020;2141:857-871
Authors: Mate?ko-Burmann I, Burmann BM
Abstract
At the foundation of many cellular processes as well as a large number of diseases is the (mis)folding of important intrinsically disordered proteins (IDPs). Despite tremendous scientific efforts, the factors driving their structural changes within the cellular context remain poorly understood. In-cell NMR...
Visualizing RNA-protein Interactions in Live Human Cells - Bioscience Technology
Visualizing RNA-protein Interactions in Live Human Cells - Bioscience Technology
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Visualizing RNA-protein Interactions in Live Human Cells
Bioscience Technology
UAlbany chemists Alan Chen Ph.D, Maksim Royzen Ph.D, and Alex Shekhtman Ph.D have combined their labs on a collaborative project aimed at visualizing RNA-protein interactions inside live human cells by using multi-dimensional NMR (nuclear ...
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[NMR paper] Characterization of proteins by in-cell NMR spectroscopy in cultured mammalian cells.
Characterization of proteins by in-cell NMR spectroscopy in cultured mammalian cells.
Characterization of proteins by in-cell NMR spectroscopy in cultured mammalian cells.
Nat Protoc. 2016 Jun;11(6):1101-1111
Authors: Barbieri L, Luchinat E, Banci L
Abstract
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[NMR paper] Algal autolysate medium to label proteins for NMR in mammalian cells.
Algal autolysate medium to label proteins for NMR in mammalian cells.
Related Articles Algal autolysate medium to label proteins for NMR in mammalian cells.
J Biomol NMR. 2016 Apr 22;
Authors: Fuccio C, Luchinat E, Barbieri L, Neri S, Fragai M
Abstract
In-cell NMR provides structural and functional information on proteins directly inside living cells. At present, the high costs of the labeled media for mammalian cells represent a limiting factor for the development of this methodology. Here we report a protocol to prepare a...
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04-24-2016 10:29 PM
Algal autolysate medium to label proteins for NMR in mammalian cells
Algal autolysate medium to label proteins for NMR in mammalian cells
Abstract
In-cell NMR provides structural and functional information on proteins directly inside living cells. At present, the high costs of the labeled media for mammalian cells represent a limiting factor for the development of this methodology. Here we report a protocol to prepare a homemade growth medium from Spirulina platensis autolysate, suitable to express uniformly labeled proteins inside mammalian cells at a reduced cost-per-sample. The human proteins SOD1 and Mia40 were...
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[NMR paper] Fluorine NMR-based assay in living mammalian cells.
Fluorine NMR-based assay in living mammalian cells.
http://www.bionmr.com//www.ncbi.nlm.nih.gov/corehtml/query/egifs/http:--linkinghub.elsevier.com-ihub-images-PubMedLink.gif Related Articles Fluorine NMR-based assay in living mammalian cells.
Anal Biochem. 2015 Dec 11;
Authors: Veronesi M, Giacomina F, Romeo E, Castellani B, Ottonello G, Lambruschini C, Garau G, Scarpelli R, Bandiera T, Piomelli D, Dalvit C
Abstract
Nuclear Magnetic Resonance (NMR)-based screening has been recognized as a powerful approach for the identification...
Visualizing Proteins in Mammalian Cells by 19F NMR spectroscopy
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