Techniques for atomic-resolution structural biology have evolved during the past several decades. Breakthroughs in instrumentation, sample preparation, and data analysis that occurred in the past decade have enabled characterization of viruses with an unprecedented level of detail. Here we review the recent advances in magic-angle spinning (MAS) nuclear magnetic resonance (NMR) spectroscopy for structural analysis of viruses and viral assemblies. MAS NMR is a powerful method that yields...
[NMR paper] Protein structural dynamics by Magic-Angle Spinning NMR
Protein structural dynamics by Magic-Angle Spinning NMR
Magic-Angle Spinning (MAS) Nuclear Magnetic Resonance (NMR) is a fast-developing technique, capable of complementing solution NMR, X-ray crystallography, and electron microscopy for the biophysical characterization of microcrystalline, poorly crystalline or disordered protein samples, such as enzymes, biomolecular assemblies, membrane-embedded systems or fibrils. Beyond structures, MAS NMR is an ideal tool for the investigation of dynamics, since it is unique in its ability to distinguish static...
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04-30-2021 10:13 PM
Accuracy and precision of protein structures determined by magic angle spinning NMR spectroscopy: for some â??with a little help from a friendâ??
Accuracy and precision of protein structures determined by magic angle spinning NMR spectroscopy: for some â??with a little help from a friendâ??
Abstract
We present a systematic investigation into the attainable accuracy and precision of protein structures determined by heteronuclear magic angle spinning solid-state NMR for a set of four proteins of varied size and secondary structure content. Structures were calculated using synthetically generated random sets of C-C distances up to 7Â*Ã? at different degrees of completeness. For single-domain...
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03-24-2019 10:41 PM
[NMR paper] Dynamics and Rigidity of an Intact Filamentous Bacteriophage Virus Probed by Magic Angle Spinning NMR.
Dynamics and Rigidity of an Intact Filamentous Bacteriophage Virus Probed by Magic Angle Spinning NMR.
Related Articles Dynamics and Rigidity of an Intact Filamentous Bacteriophage Virus Probed by Magic Angle Spinning NMR.
Chemistry. 2018 Apr 16;:
Authors: Aharoni T, Goldbourt A
Abstract
The capsid dynamics of filamentous bacteriophages is related to their function, stability and interactions with the genome, and can be assessed by measuring the chemical shift anisotropy (CSA) of 15N-amides, which are sensitive to large amplitude...
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04-17-2018 11:55 AM
Peptide and Protein Dynamics and Low-Temperature/DNP Magic Angle Spinning NMR #DNPNMR
From The DNP-NMR Blog:
Peptide and Protein Dynamics and Low-Temperature/DNP Magic Angle Spinning NMR #DNPNMR
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Ni, Q.Z., et al., Peptide and Protein Dynamics and Low-Temperature/DNP Magic Angle Spinning NMR. The Journal of Physical Chemistry B, 2017. 121(19): p. 4997-5006.
http://dx.doi.org/10.1021/acs.jpcb.7b02066
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08-29-2017 01:33 AM
Peptide and Protein Dynamics and Low-Temperature/DNP Magic Angle Spinning NMR #DNPNMR
From The DNP-NMR Blog:
Peptide and Protein Dynamics and Low-Temperature/DNP Magic Angle Spinning NMR #DNPNMR
p.p1 {margin: 0.0px 0.0px 0.0px 36.0px; text-indent: -36.0px; font: 12.0px Helvetica}
Ni, Q.Z., et al., Peptide and Protein Dynamics and Low-Temperature/DNP Magic Angle Spinning NMR. J Phys Chem B, 2017. 121(19): p. 4997-5006.
https://www.ncbi.nlm.nih.gov/pubmed/28437077
nmrlearner
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07-07-2017 03:20 PM
Peptide and Protein Dynamics and Low-Temperature/DNP Magic Angle Spinning NMR #DNPNMR
From The DNP-NMR Blog:
Peptide and Protein Dynamics and Low-Temperature/DNP Magic Angle Spinning NMR #DNPNMR
p.p1 {margin: 0.0px 0.0px 0.0px 36.0px; text-indent: -36.0px; font: 12.0px Helvetica}
Ni, Q.Z., et al., Peptide and Protein Dynamics and Low-Temperature/DNP Magic Angle Spinning NMR. J Phys Chem B, 2017.
https://www.ncbi.nlm.nih.gov/pubmed/28437077
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05-10-2017 09:55 PM
[NMR paper] Peptide and Protein Dynamics and Low-Temperature/DNP Magic Angle Spinning NMR.
Peptide and Protein Dynamics and Low-Temperature/DNP Magic Angle Spinning NMR.
Related Articles Peptide and Protein Dynamics and Low-Temperature/DNP Magic Angle Spinning NMR.
J Phys Chem B. 2017 Apr 24;:
Authors: Ni QZ, Markhasin E, Can TV, Corzilius B, Tan KO, Barnes AB, Daviso E, Su Y, Herzfeld J, Griffin RG
Abstract
In DNP MAS NMR experiments at ~80-110 K, the structurally important -13CH3 and -15NH3+ signals in MAS spectra of biological samples disappear due to the interference of the molecular motions with the 1H...
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04-25-2017 11:48 AM
[NMR paper] Investigation of the Curvature Induction and Membrane Localization of the Influenza Virus M2 Protein Using Static and Off-Magic-Angle Spinning Solid-State NMR of Oriented Bicelles.
Investigation of the Curvature Induction and Membrane Localization of the Influenza Virus M2 Protein Using Static and Off-Magic-Angle Spinning Solid-State NMR of Oriented Bicelles.
Investigation of the Curvature Induction and Membrane Localization of the Influenza Virus M2 Protein Using Static and Off-Magic-Angle Spinning Solid-State NMR of Oriented Bicelles.
Biochemistry. 2015 Mar 16;
Authors: Wang T, Hong M
Abstract
A wide variety of membrane proteins induce membrane curvature for function, thus it is important to develop...
Virus Structures and Dynamics by Magic-Angle Spinning NMR
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