Lyrelle S. Lloyd, Ralph W. Adams, Michael Bernstein, Steven Coombes, Simon B. Duckett, Gary G. R. Green, Richard. J. Lewis, Ryan E. Mewis and Christopher J. Sleigh
Journal of the American Chemical Society
DOI: 10.1021/ja3051052
[Question from NMRWiki Q&A forum] Finding concentration from NMR spectrum
Finding concentration from NMR spectrum
I dissolve Aspartate in a buffer containing 2mM TSP. I have a NMR spectrum of the mixture and I need to find the concentration for the Aspartate.
The 1D spectrum shows 7 peaks at (ppm):0.00 (0.505), 0.60 (0.106), 1.75 (0.124), 2.62 (1.012), 2.80 (1.000), 3.65 (0.034), 3.80 (1.000)
What is the best way to find the concentration?
nmrlearner
News from other NMR forums
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06-06-2012 06:03 AM
[NMR900 blog] Review in Nature Methods
Review in Nature Methods
J.R. Bothe, E.N. Nikolova, C.D. Eichhorn, J. Chugh, A.L. Hansen and H.M. Al-Hashimi, "Characterizing RNA dynamics at atomic resolution using solution-state NMR spectroscopy," Nature Methods 8 (2011) 919–931. (Review) http://dx.doi.org/10.1038/nmeth.1735
https://blogger.googleusercontent.com/tracker/8663203727601106205-1377108512543150595?l=nmr900.blogspot.com
Read complete story on NMR900 blog
nmrlearner
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NMR-based methods and strategies for drug discovery.
Related Articles NMR-based methods and strategies for drug discovery.
Chem Soc Rev. 2003 Nov;32(6):365-72
Authors: Salvatella X, Giralt E
Nuclear Magnetic Resonance (NMR) spectroscopy has long been a favourite tool of chemists interested in host-guest systems because it permits access to a wealth of information about the molecular recognition reaction. NMR has evolved dramatically in the last 15 years and, in parallel with the development of NMR methods for the determination of protein...
nmrlearner
Journal club
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11-24-2010 09:16 PM
[NMR900 blog] Multidimensional NMR Methods for the Solution State
Multidimensional NMR Methods for the Solution State
edited by Gareth A. Morris and James W. Emsley
Hardcover: 580 pages
Publisher: Wiley; June 2010
Language: English
ISBN: 978-0470770757
http://www.amazon.com/dp/0470770759
http://www.amazon.ca/dp/0470770759
nmrlearner
News from NMR blogs
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08-22-2010 02:30 AM
[NMR900 blog] Multidimensional NMR Methods for the Solution State
Multidimensional NMR Methods for the Solution State
edited by Gareth A. Morris and James W. Emsley
Hardcover: 580 pages
Publisher: Wiley; June 2010
Language: English
ISBN: 978-0470770757
http://www.amazon.com/dp/0470770759
http://www.amazon.ca/dp/0470770759
nmrlearner
News from NMR blogs
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08-22-2010 02:18 AM
[NMR paper] Estimation of the intracellular free ADP concentration by 19F NMR studies of fluorine
Estimation of the intracellular free ADP concentration by 19F NMR studies of fluorine-labeled yeast phosphoglycerate kinase in vivo.
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Biochemistry. 1993 May 11;32(18):4895-902
Authors: Williams SP, Fulton AM, Brindle KM
Yeast phosphoglycerate kinase was selectively fluorine-labeled in vivo by inducing enzyme synthesis in stationary phase cells in the presence of 5-fluorotryptophan. Inducible...
nmrlearner
Journal club
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08-21-2010 11:53 PM
Online help for measurement concentration by NMR
Below are links to online resources that can help students to understand how to measure concentration of substances with NMR. If you know websites that discuss this issue, please post links to them in this thread. Quantitative Nuclear Magnetic Resonance, Chemistry 3113, University of North Carolina Charlotte
Chem152, Rice University
Relevant softwareACD/Combi NMR
Utilization of SABRE-DerivedHyperpolarization ToDetect Low-Concentration Analytes via 1D and 2D NMR Methods
Linear Mode
