[NMR paper] Easy-to-Attach/Detach Solubilizing Tag-Aided Chemical Synthesis of an Aggregative Capsid Protein
Easy-to-Attach/Detach Solubilizing Tag-Aided Chemical Synthesis of an Aggregative Capsid Protein
A solubilizing "Trt-K10" tag was developed for the effective chemical preparation of peptides/proteins with low solubility. The Trt-K10 tag comprises a hydrophilic oligo-Lys sequence and a trityl anchor, and can be selectively introduced to a side chain thiol of Cys of deprotected peptides/proteins with a trityl alcohol-type introducing reagent "Trt(OH)-K10" under acidic conditions. Significantly, the ligation product in the reaction mixture of a thiol-additive-free native chemical ligation...
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01-09-2018 09:41 AM
[NMR paper] P-B desulfurization: an enabling method for protein chemical synthesis and site-specific deuteration
P-B desulfurization: an enabling method for protein chemical synthesis and site-specific deuteration
Native chemical ligation has provided a powerful tool for protein chemical synthesis. Herein, we report an unprecedented mild system (TCEP-NaBH4 or TCEP-LiBEt3H) for chemoselective peptide desulfurization to achieve effective protein synthesis via the native chemical ligation-desulfurization approach. This method, termed P-B desulfurization, features usage of common reagents, simplicity of operation, robustness, high yielding, clean conversion and versatile functionality compatibility...
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10-03-2017 03:24 AM
[NMR paper] Chemical Synthesis of the Highly Hydrophobic Antiviral Membrane Associated Protein IFITM3 and Modified Variants
Chemical Synthesis of the Highly Hydrophobic Antiviral Membrane Associated Protein IFITM3 and Modified Variants
Interferon-induced transmembrane protein 3 (IFITM3) is an antiviral transmembrane protein thought to serve as the primary factor for inhibiting replication of a large number of viruses, including the West Nile virus, the Dengue virus, the Ebola virus, and the Zika virus. Production of this 14.5 kDa, 133-residue, transmembrane protein, especially with essential posttranslational modifications by recombinant expression is challenging. In this report, we document the chemical...
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08-21-2017 02:23 PM
[NMR paper] Palladium in the Chemical Synthesis and Modification of Proteins
Palladium in the Chemical Synthesis and Modification of Proteins
The field of site-specific modification of proteins has drawn significant attention in recent years owing to its importance in various research areas such as the development of novel therapeutics and understanding the biochemical and cellular behaviors of proteins. The presence of a large number of reactive functional groups in the protein of interest and in the cellular environment renders modification at a specific site a highly challenging task. With the development of sophisticated chemical methodologies it is now...
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08-07-2017 07:31 PM
[NMR paper] Palladium in Chemical Protein Synthesis and Modifications
Palladium in Chemical Protein Synthesis and Modifications
The field of site-specific modification of proteins has drawn significant attentions in recent years owing to its high importance in various research areas such as the development of novel therapeutics and understanding the biochemical and cellular behaviors of proteins. The presence of a large number of reactive functional groups in the protein of interest and in the cellular environment renders the particular modification at a specific site a highly challenging task. However, with the development of sophisticated...
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04-07-2017 02:01 AM
[NMR paper] Isotope-Labeled Amyloids via Synthesis, Expression, and Chemical Ligation for Use in FTIR, 2D IR, and NMR Studies.
Isotope-Labeled Amyloids via Synthesis, Expression, and Chemical Ligation for Use in FTIR, 2D IR, and NMR Studies.
Related Articles Isotope-Labeled Amyloids via Synthesis, Expression, and Chemical Ligation for Use in FTIR, 2D IR, and NMR Studies.
Methods Mol Biol. 2016;1345:21-41
Authors: Zhang TO, Grechko M, Moran SD, Zanni MT
Abstract
This chapter provides protocols for isotope-labeling the human islet amyloid polypeptide (hIAPP or amylin) involved in type II diabetes and ?D-crystallin involved in cataract formation. Because...
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10-12-2015 01:04 AM
[NMR paper] Synthesis of modified Trichinella spiralis disaccharide epitopes and a comparison of their recognition by chemical mapping and saturation transfer difference NMR.
Synthesis of modified Trichinella spiralis disaccharide epitopes and a comparison of their recognition by chemical mapping and saturation transfer difference NMR.
Synthesis of modified Trichinella spiralis disaccharide epitopes and a comparison of their recognition by chemical mapping and saturation transfer difference NMR.
Carbohydr Res. 2013 Nov 1;383C:1-13
Authors: Cui L, Ling CC, Sadowska J, Bundle DR
Abstract
A rat monoclonal antibody 9D4 raised against the cell surface N-glycan of the parasite Trichinella spirallis protects rats...
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11-20-2013 12:42 AM
[NMR paper] Synthesis of isotope labeled oligonucleotides and their use in an NMR study of a prot
Synthesis of isotope labeled oligonucleotides and their use in an NMR study of a protein-DNA complex.
http://www.ncbi.nlm.nih.gov/corehtml/query/egifs/http:--www.pubmedcentral.nih.gov-corehtml-pmc-pmcgifs-pubmed-pmc.gif Related Articles Synthesis of isotope labeled oligonucleotides and their use in an NMR study of a protein-DNA complex.
Nucleic Acids Res. 1992 Feb 25;20(4):653-7
Authors: Kellenbach ER, Remerowski ML, Eib D, Boelens R, van der Marel GA, van den Elst H, van Boom JH, Kaptein R
The synthesis of an oligonucleotide labeled with 13C...
Using Chemical Synthesis To Study and Apply Protein Glycosylation
Linear Mode
