Related ArticlesUsing 19F NMR to Probe Biological Interactions of Proteins and Peptides.
ACS Chem Biol. 2014 Apr 24;
Authors: Marsh EN, Suzuki Y
Abstract
Fluorine is a valuable probe for investigating the interactions of biological molecules because of its favorable NMR characteristics, its small size and its near total absence from biology. Advances in biosynthetic methods allow fluorine to be introduced into peptides and proteins with high precision, and the increasing sensitivity of NMR spectrometers has facilitated the use of 19F NMR to obtain molecular-level insights into a wide range of often-complex biological interactions. Here we summarize the advantages of 19F NMR for studying the interactions of peptides and proteins, review methods for the production of fluorine-labeled materials, and describe some representative recent examples in which 19F NMR has been used to study conformational changes in peptides and proteins and their interactions with other biological molecules.
PMID: 24762032 [PubMed - as supplied by publisher]
[NMR paper] (19)F-NMR screening of unrelated antimicrobial peptides shows that membrane interactions are largely governed by lipids.
(19)F-NMR screening of unrelated antimicrobial peptides shows that membrane interactions are largely governed by lipids.
(19)F-NMR screening of unrelated antimicrobial peptides shows that membrane interactions are largely governed by lipids.
Biochim Biophys Acta. 2014 Mar 31;
Authors: Afonin S, Glaser RW, Sachse C, Salgado J, Wadhwani P, Ulrich AS
Abstract
Many amphiphilic antimicrobial peptides permeabilize bacterial membranes via successive steps of binding, re-alignment and/or oligomerization. Here, we have systematically...
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04-06-2014 02:01 AM
[NMR paper] NMR studies of Interactions between Bax and BH3 domain-containing peptides in the absence and presence of CHAPS.
NMR studies of Interactions between Bax and BH3 domain-containing peptides in the absence and presence of CHAPS.
NMR studies of Interactions between Bax and BH3 domain-containing peptides in the absence and presence of CHAPS.
Arch Biochem Biophys. 2014 Jan 13;
Authors: Yao S, Westphal D, Babon JJ, Thompson GV, Robin AY, Adams JM, Colman PM, Czabotar PE
Abstract
Activation and oligomerisation of Bax, a key pro-apoptotic Bcl-2 family protein, are key steps in the mitochondrial pathway to apoptosis. The signals for apoptosis are conveyed by...
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01-18-2014 11:31 AM
[NMR paper] (19) F NMR Spectroscopy as a Probe of Cytoplasmic Viscosity and Weak Protein Interactions in Living Cells.
(19) F NMR Spectroscopy as a Probe of Cytoplasmic Viscosity and Weak Protein Interactions in Living Cells.
(19) F NMR Spectroscopy as a Probe of Cytoplasmic Viscosity and Weak Protein Interactions in Living Cells.
Chemistry. 2013 Aug 6;
Authors: Ye Y, Liu X, Zhang Z, Wu Q, Jiang B, Jiang L, Zhang X, Liu M, Pielak GJ, Li C
Abstract
Protein mobility in living cells is vital for cell function. Both cytosolic viscosity and weak protein-protein interactions affect mobility, but examining viscosity and weak interaction effects is challenging....
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08-08-2013 03:46 PM
The Chemoselective Reactions of Tyrosine-Containing G-Protein-Coupled Receptor Peptides with [Cp*Rh(H2O)3](OTf)2, Including 2D NMR Structures and the Biological Consequences
The Chemoselective Reactions of Tyrosine-Containing G-Protein-Coupled Receptor Peptides with (OTf)2, Including 2D NMR Structures and the Biological Consequences
H. Bauke Albada, Florian Wieberneit, Ingrid Dijkgraaf, Jessica H. Harvey, Jennifer L. Whistler, Raphael Stoll, Nils Metzler-Nolte and Richard H. Fish
http://pubs.acs.org/appl/literatum/publisher/achs/journals/content/jacsat/0/jacsat.ahead-of-print/ja303010k/aop/images/medium/ja-2012-03010k_0004.gif
Journal of the American Chemical Society
DOI: 10.1021/ja303010k
http://feeds.feedburner.com/~ff/acs/jacsat?d=yIl2AUoC8zA ...
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06-16-2012 06:01 AM
[Question from NMRWiki Q&A forum] Tuning probe failed after a dual probe was replaced with a BBI probe
Tuning probe failed after a dual probe was replaced with a BBI probe
We generally use Dual to run 13C and BBI to run 2D. After changed the probe, the command "edhead" was used to set the probe. Put the sample tube, lock the solvent, and then type "atma" to tune the probe. We always do it like this, but now we can not tune the proton after installed the BBI probe (13C is OK). The dip can not be found by "atma", and "atmm" was also not work on forming a dip. What is the most possible reason for this error? How to solve it and avoid it in the future ? Thanks. (Instrument: Bruker 400 MHz,...
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08-23-2011 05:31 PM
[NMR paper] The interactions of the HIV gp41 fusion peptides with zwitterionic membrane mimics de
The interactions of the HIV gp41 fusion peptides with zwitterionic membrane mimics determined by NMR spectroscopy.
Related Articles The interactions of the HIV gp41 fusion peptides with zwitterionic membrane mimics determined by NMR spectroscopy.
Biochim Biophys Acta. 2004 Nov 17;1667(1):67-81
Authors: Morris KF, Gao X, Wong TC
The wild-type (wt) N-terminal 23-residue fusion peptide (FP) of the human immunodeficiency virus (HIV) fusion protein gp41 and its V2E mutant have been studied by nuclear magnetic resonance (NMR) spectroscopy in...
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11-24-2010 10:03 PM
[Question from NMRWiki Q&A forum] Please suggest model proteins and peptides for NMR
Please suggest model proteins and peptides for NMR
do you know any model proteins except lysozyme suitable for nmr experiments?
Check if somebody has answered this question on NMRWiki QA forum
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08-22-2010 02:30 AM
[Question from NMRWiki Q&A forum] When to label peptides and proteins and when not?
When to label peptides and proteins and when not?
When pursuing an NMR structure project - at what size of oligopeptide you would decide to label it with:
15N
13C
both
Using 19F NMR to Probe Biological Interactions of Proteins and Peptides.
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