Useof 77Se and 125Te NMR Spectroscopyto Probe Covalency of the Actinide-Chalcogen Bonding in [Th(En){N(SiMe3)2}3]- (E = Se, Te; n = 1, 2) andTheir Oxo-Uranium(VI) Congeners
Useof 77Se and 125Te NMR Spectroscopyto Probe Covalency of the Actinide-Chalcogen Bonding in [Th(En){N(SiMe3)2}3]- (E = Se, Te; n = 1, 2) andTheir Oxo-Uranium(VI) Congeners
[NMR paper] 77Se Chemical Shift Tensor of L-selenocystine: Experimental NMR Measurements and Quantum Chemical Investigations of Structural Effects.
77Se Chemical Shift Tensor of L-selenocystine: Experimental NMR Measurements and Quantum Chemical Investigations of Structural Effects.
Related Articles 77Se Chemical Shift Tensor of L-selenocystine: Experimental NMR Measurements and Quantum Chemical Investigations of Structural Effects.
J Phys Chem B. 2015 Feb 5;
Authors: Struppe JO, Zhang Y, Rozovsky S
Abstract
The genetically encoded amino acid selenocysteine and its dimeric form, selenocystine, are both utilized by nature. They are found in active sites of...
77Se Enrichment of Proteins Expands the Biological NMR Toolbox
77Se Enrichment of Proteins Expands the Biological NMR Toolbox
23 January 2013
Publication year: 2013
Source:Journal of Molecular Biology, Volume 425, Issue 2</br>
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Sulfur, a key contributor to biological reactivity, is not amendable to investigations by biological NMR spectroscopy. To utilize selenium as a surrogate, we have developed a generally applicable 77Se isotopic enrichment method for heterologous proteins expressed in Escherichia coli. We demonstrate 77Se NMR spectroscopy of multiple selenocysteine and selenomethionine residues in the sulfhydryl oxidase...
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02-03-2013 10:13 AM
[Question from NMRWiki Q&A forum] Tuning probe failed after a dual probe was replaced with a BBI probe
Tuning probe failed after a dual probe was replaced with a BBI probe
We generally use Dual to run 13C and BBI to run 2D. After changed the probe, the command "edhead" was used to set the probe. Put the sample tube, lock the solvent, and then type "atma" to tune the probe. We always do it like this, but now we can not tune the proton after installed the BBI probe (13C is OK). The dip can not be found by "atma", and "atmm" was also not work on forming a dip. What is the most possible reason for this error? How to solve it and avoid it in the future ? Thanks. (Instrument: Bruker 400 MHz,...
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08-23-2011 05:31 PM
[NMR paper] NMR analysis of the hydrogen bonding interactions of the RNA-binding domains of the D
NMR analysis of the hydrogen bonding interactions of the RNA-binding domains of the Drosophila sex-lethal protein with target RNA fragments with site-specific uridine substitutions.
http://www.ncbi.nlm.nih.gov/corehtml/query/egifs/http:--highwire.stanford.edu-icons-externalservices-pubmed-custom-oxfordjournals_final_free.gif http://www.ncbi.nlm.nih.gov/corehtml/query/egifs/http:--www.pubmedcentral.nih.gov-corehtml-pmc-pmcgifs-pubmed-pmc.gif Related Articles NMR analysis of the hydrogen bonding interactions of the RNA-binding domains of the Drosophila sex-lethal protein with...
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08-22-2010 03:31 PM
[NMR paper] 77Se NMR characterization of 77Se-labeled ovine erythrocyte glutathione peroxidase.
77Se NMR characterization of 77Se-labeled ovine erythrocyte glutathione peroxidase.
Related Articles 77Se NMR characterization of 77Se-labeled ovine erythrocyte glutathione peroxidase.
J Biol Chem. 1991 Mar 15;266(8):4804-9
Authors: Gettins P, Crews BC
Lambs, maintained on a selenium-deficient diet supplemented with 94 atom % Na2 27SeO3, have been used as a source of 77Se-enriched erythrocyte glutathione peroxidase. After 5 months on this diet, the percentage of selenium in the enzyme derived from the supplement had reached 88%. From each...
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08-21-2010 11:16 PM
[NMR paper] NMR relaxation properties of 77Se-labeled proteins.
NMR relaxation properties of 77Se-labeled proteins.
Related Articles NMR relaxation properties of 77Se-labeled proteins.
J Biol Chem. 1991 Feb 25;266(6):3422-6
Authors: Gettins P, Wardlaw SA
A 77Se-containing moiety has been attached to cysteine residues in bovine hemoglobin, reduced ribonuclease A, and glutathione by reaction with 6,6'-diselenobis(3-nitrobenzoic acid). The resultant species contain Se-S linkages that have 77Se NMR absorptions in the range range of 568-580 ppm. Spectra have been recorded at 4.7 and 9.7 tesla (T). For labeled...
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08-21-2010 11:16 PM
Hydrogen-bonding potential to refine NMR structure
An Empirical Backbone-Backbone Hydrogen-Bonding Potential in Proteins and Its Applications to NMR Structure Refinement and Validation
Alexander Grishaev and Ad Bax
J. Am. Chem. Soc.; 2004; 126(23) pp 7281 - 7292
http://pubs.acs.org./isubscribe/journals/jacsat/126/i23/figures/ja0319994n00001.gif
Abstract:
A new multidimensional potential is described that encodes for the relative spatial arrangement of the peptidyl backbone units as observed within a large database of high-resolution X-ray structures. The detailed description afforded by such an analysis provides an opportunity to study...
![Useof 77Se and 125Te NMR Spectroscopyto Probe Covalency of the Actinide-Chalcogen Bonding in [Th(En){N(SiMe3)2}3]- (E = Se, Te; n = 1, 2) andTheir Oxo-Uranium(VI) Congeners](http://www.bionmr.com/forum/iconimages/journal-club-9/useof-77se-125te-nmr-spectroscopyto-probe-covalency-actinide-chalcogen-bonding-%5Bth-en-%7Bn-sime3-2%7D3%5D-e-%3D-se-te%3B-n-%3D-1-2-andtheir-oxo-uranium-vi-congeners_ltr.gif)
Useof 77Se and 125Te NMR Spectroscopyto Probe Covalency of the Actinide-Chalcogen Bonding in [Th(En){N(SiMe3)2}3]- (E = Se, Te; n = 1, 2) andTheir Oxo-Uranium(VI) Congeners
Linear Mode
