NMR paper On the use of ultracentrifugal devices for sedimented solute NMR.

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[NMR paper] On the use of ultracentrifugal devices for sedimented solute NMR.

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02-13-2013, 12:47 PM

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On the use of ultracentrifugal devices for sedimented solute NMR.

Related Articles On the use of ultracentrifugal devices for sedimented solute NMR.

J Biomol NMR. 2012 Oct;54(2):123-7

Authors: Bertini I, Engelke F, Gonnelli L, Knott B, Luchinat C, Osen D, Ravera E

Abstract
We have recently proposed sedimented solute NMR (SedNMR) as a solid-state method to access biomolecules without the need of crystallization or other sample manipulation. The drawback of SedNMR is that samples are intrinsically diluted and this is detrimental for the signal intensity. Ultracentrifugal devices can be used to increase the amount of sample inside the rotor, overcoming the intrinsic sensitivity limitation of the method. We designed two different devices and we here report the directions for using such devices and the relevant equations for determining the parameters for sedimentation.

PMID: 22872367 [PubMed - indexed for MEDLINE]

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