[NMR paper] Use of a combination of the MD simulations and NMR spectroscopy to determine the regulatory mechanism of pulsed electric field (PEF) targeting at C-terminal histidine of VNAVLH.
Use of a combination of the MD simulations and NMR spectroscopy to determine the regulatory mechanism of pulsed electric field (PEF) targeting at C-terminal histidine of VNAVLH.
Related ArticlesUse of a combination of the MD simulations and NMR spectroscopy to determine the regulatory mechanism of pulsed electric field (PEF) targeting at C-terminal histidine of VNAVLH.
Food Chem. 2021 Jan 01;334:127554
Authors: Zhang S, Dong L, Sun L, Yang Y, Zhang S, Lin S
Abstract
In this study, the targeted regulatory mechanism of pulsed electric field (PEF) was explored for antioxidant activity improvement in four peptides, RGAVIH, RGAVLH, VNAVIH, and VNAVLH, of the pine nut (Pinus koraiensis Sieb. et Zucc). The VNAVLH peptide exhibited the best antioxidant activity and the ?-sheet content decreased to a minimum value at 40*kV/cm. Moreover, the chemical shifts of hydrogen atoms of 2-H Asn and 6-H His shifted to a higher magnetic field. The connectivity between N?H (3.62*ppm) and C?H (8.10*ppm) of 6-His residue disappeared in PEF-treated peptide. Molecule dynamics (MD) simulation verified that the distances of N?(H78)-C?(H80) and H82-O94 increased, whereas -OH and -C?(H83) got closer in histidine residue after applying the electric field force. Therefore, the antioxidant activity enhancement of VNAVLH might due to the targeted regulation of PEF treatment on N?H-C?H and imidazole group in histidine.
[NMR paper] Protein structural changes characterized by high-pressure, pulsed field gradient diffusion NMR spectroscopy.
Protein structural changes characterized by high-pressure, pulsed field gradient diffusion NMR spectroscopy.
http://www.bionmr.com//www.ncbi.nlm.nih.gov/corehtml/query/egifs/https:--linkinghub.elsevier.com-ihub-images-PubMedLink.gif Related Articles Protein structural changes characterized by high-pressure, pulsed field gradient diffusion NMR spectroscopy.
J Magn Reson. 2020 Feb 19;312:106701
Authors: Ramanujam V, Alderson TR, Pritišanac I, Ying J, Bax A
Abstract
Pulsed-field gradient NMR spectroscopy is widely used to measure...
Molecular Simulations Reveal an Unresolved Conformationof the Type IA Protein Kinase A Regulatory Subunit and Suggest ItsRole in the cAMP Regulatory Mechanism
Molecular Simulations Reveal an Unresolved Conformationof the Type IA Protein Kinase A Regulatory Subunit and Suggest ItsRole in the cAMP Regulatory Mechanism
http://pubs.acs.org/appl/literatum/publisher/achs/journals/content/bichaw/0/bichaw.ahead-of-print/acs.biochem.7b00461/20170717/images/medium/bi-2017-00461q_0003.gif
Biochemistry
DOI: 10.1021/acs.biochem.7b00461
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[NMR paper] Use of a combination of the RDC method and NOESY NMR spectroscopy to determine the structure of Alzheimer's amyloid A?10-35 peptide in solution and in SDS micelles.
Use of a combination of the RDC method and NOESY NMR spectroscopy to determine the structure of Alzheimer's amyloid A?10-35 peptide in solution and in SDS micelles.
http://www.bionmr.com//www.ncbi.nlm.nih.gov/corehtml/query/egifs/http:--production.springer.de-OnlineResources-Logos-springerlink.gif Related Articles Use of a combination of the RDC method and NOESY NMR spectroscopy to determine the structure of Alzheimer's amyloid A?10-35 peptide in solution and in SDS micelles.
Eur Biophys J. 2013 Dec;42(11-12):803-10
Authors: Usachev KS, Filippov AV,...
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Discovered a new regulatory mechanism of c-Src, the human protein bound to ... - HealthCanal.com
Discovered a new regulatory mechanism of c-Src, the human protein bound to ... - HealthCanal.com
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Discovered a new regulatory mechanism of c-Src, the human protein bound to ...
HealthCanal.com
... leader of the Biomolecular Nuclear Magnetic Resonance (NMR) Research Group, affiliated with the Department of Organic Chemistry of the UB, â??the discovery of a new regulatory mechanism in such a relevant and extensively studied protein proves the ...
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[NMR paper] Use of 1H NMR spectroscopy and computer simulations To analyze histidine pKa changes
Use of 1H NMR spectroscopy and computer simulations To analyze histidine pKa changes in a protein tyrosine phosphatase: experimental and theoretical determination of electrostatic properties in a small protein.
http://www.ncbi.nlm.nih.gov/corehtml/query/egifs/http:--pubs.acs.org-images-acspubs.jpg Related Articles Use of 1H NMR spectroscopy and computer simulations To analyze histidine pKa changes in a protein tyrosine phosphatase: experimental and theoretical determination of electrostatic properties in a small protein.
Biochemistry. 1997 Sep 30;36(39):11984-94
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[NMR paper] Measuring protein self-association using pulsed-field-gradient NMR spectroscopy: appl
Measuring protein self-association using pulsed-field-gradient NMR spectroscopy: application to myosin light chain 2.
Related Articles Measuring protein self-association using pulsed-field-gradient NMR spectroscopy: application to myosin light chain 2.
J Biomol NMR. 1995 Nov;6(3):321-8
Authors: Dingley AJ, Mackay JP, Chapman BE, Morris MB, Kuchel PW, Hambly BD, King GF
At the millimolar concentrations required for structural studies, NMR spectra of the calcium-binding protein myosin light chain 2 (MLC2) showed resonance line widths indicative...
Use of a combination of the MD simulations and NMR spectroscopy to determine the regulatory mechanism of pulsed electric field (PEF) targeting at C-terminal histidine of VNAVLH.
Linear Mode
