NMR paper Unraveling long range residual dipolar coupling networks in strongly aligned proteins

		BioNMR > Educational resources > Journal club
[NMR paper] Unraveling long range residual dipolar coupling networks in strongly aligned proteins

Webservers

NMR processing:

MDD

NMR assignment:

Backbone:

Side-chains:

NOEs:

Structure from NMR restraints:

Ab initio:

Fragment-based:

Rosetta-NMR (Robetta)

Template-based:

GeNMR

I-TASSER

Refinement:

Amber

Structure from chemical shifts:

Fragment-based:

Homology-based:

Torsion angles from chemical shifts:

Preditor

TALOS

Promega- Proline

Secondary structure from chemical shifts:

CSI (via RCI server)

TALOS

MICS caps, β-turns

d2D

PECAN

Flexibility from chemical shifts:

RCI

Interactions from chemical shifts:

HADDOCK

Chemical shifts re-referencing:

NMR model quality:

NOEs, other restraints:

Chemical shifts:

RDCs:

Pseudocontact shifts:

Protein geomtery:

SAVES2 or SAVES4

Quality Control Check

NMR spectrum prediction:

FANDAS

MestReS

V-NMR

Flexibility from structure:

Backbone S2

Methyl S2

B-factor

Molecular dynamics:

Gromacs

Amber

Antechamber

Chemical shifts prediction:

From structure:

Shiftx2

Sparta+

Camshift

CH3shift- Methyl

ArShift- Aromatic

ShiftS

Proshift

PPM

CheShift-2- Cα

From sequence:

Shifty

Camcoil

Poulsen_rc_CS

Disordered proteins:

MAXOCC

Format conversion & validation:

CCPN

From NMR-STAR 3.1

Validate NMR-STAR 3.1

NMR sample preparation:

Protein disorder:

DisMeta

Protein solubility:

Isotope labeling:

Solid-state NMR:

Thread Tools

Search this Thread

Rate Thread

Display Modes

07-11-2013, 12:07 PM

nmrlearner

Senior Member

Join Date: Jan 2005

Posts: 23,734

Points: 193,617, Level: 100

Level up: 0%, 0 Points needed

Activity: 50.7%

Last Achievements

Award-Showcase

NMR Credits: 0

NMR Points: 193,617

Downloads: 0

Uploads: 0

Unraveling long range residual dipolar coupling networks in strongly aligned proteins

Unraveling long range residual dipolar coupling networks in strongly aligned proteins

Publication date: Available online 10 July 2013
Source:Journal of Magnetic Resonance

Author(s): Luke Arbogast , Ananya Majumdar , Joel R. Tolman

Long-range residual dipolar couplings (lrRDCs) have the potential to serve as powerful structural restraints in protein NMR spectroscopy as they can provide both distance and orientation information about nuclei separate in sequence but close in space. Current nonselective methods for their measurement are limited to moderate alignment strengths due to the sheer abundance of active couplings at stronger alignment. This limits the overall magnitude and therefore distance across which couplings can be measured. We have developed a double resonance technique for the inversion of individual coupled spin pairs, called Selective Inversion by Single Transition Cross Polarization (SIST-CP). This technique enables the selective recoupling of lrRDCs, thus allowing the complex multiplets occurring in strongly aligned systems to be disentangled. This technique is demonstrated in the context of an application to the measurement of 13C’-1HN lrRDCs in strongly aligned proteins.
Graphical abstract

Highlights

More...

Did you find this post helpful?

Similar Threads
Thread	Thread Starter	Forum	Replies	Last Post
[Question from NMRWiki Q&A forum] Long-range 1H-{15N} coupling (HMBC) Long-range 1H-{15N} coupling (HMBC) Hello All I have two examples of what appears to be 6J coupling between 1H and 15N using gradient HMBC experiments with 15N at natural abundance. For such an experiment, 2J and 3J correlations are typical, sometimes 4J (W-type) but I've never seen 6J before. Has anyone else ever seen correlations over this distance? Any other comments? Craig.	nmrlearner	News from other NMR forums	0	10-29-2012 07:03 PM
[U. of Ottawa NMR Facility Blog] Measurement of Long Range C H Coupling Constants Measurement of Long Range C H Coupling Constants The stereochemistry of compounds is assigned very often with proton - proton NOE's by applying the 2D NOESY technique or the 1D selective gradient NOESY technique. These methods fail, however when the distance between protons is too large to measure an NOE. When faced with this situation, it may be possible to measure long range proton - carbon coupling constants which are able to provide the necessary information. Three-bond carbon - proton couplings follow a Karplus relationship where the magnitude of the coupling constant is related to...	nmrlearner	News from NMR blogs	0	08-17-2012 10:44 PM
Backbone assignment of perdeuterated proteins using long-range H/C-dipolar transfers Backbone assignment of perdeuterated proteins using long-range H/C-dipolar transfers Abstract For micro-crystalline proteins, solid-state nuclear magnetic resonance spectroscopy of perdeuterated samples can provide spectra of unprecedented quality. Apart from allowing to detect sparsely introduced protons and thereby increasing the effective resolution for a series of sophisticated techniques, deuteration can provide extraordinary coherence lifetimesâ??obtainable for all involved nuclei virtually without decoupling and enabling the use of scalar magnetization transfers. Unfortunately,...	nmrlearner	Journal club	0	12-17-2011 04:44 AM
The Use of Residual Dipolar Coupling in Studying Proteins by NMR. The Use of Residual Dipolar Coupling in Studying Proteins by NMR. The Use of Residual Dipolar Coupling in Studying Proteins by NMR. Top Curr Chem. 2011 Sep 28; Authors: Chen K, Tjandra N Abstract The development of residual dipolar coupling (RDC) in protein NMR spectroscopy, over a decade ago, has become a useful and almost routine tool for accurate protein solution structure determination. RDCs provide orientation information of magnetic dipole-dipole interaction vectors within a common reference frame. Its measurement requires a...	nmrlearner	Journal club	0	09-30-2011 06:00 AM
The Use of Residual Dipolar Coupling in Studying Proteins by NMR. The Use of Residual Dipolar Coupling in Studying Proteins by NMR. The Use of Residual Dipolar Coupling in Studying Proteins by NMR. Top Curr Chem. 2011 Sep 28; Authors: Chen K, Tjandra N Abstract The development of residual dipolar coupling (RDC) in protein NMR spectroscopy, over a decade ago, has become a useful and almost routine tool for accurate protein solution structure determination. RDCs provide orientation information of magnetic dipole-dipole interaction vectors within a common reference frame. Its measurement requires a...	nmrlearner	Journal club	0	09-30-2011 05:59 AM
Paramagnetic-Based NMR Restraints Lift Residual Dipolar Coupling Degeneracy in Multidomain Detergent-Solubilized Membrane Proteins. Paramagnetic-Based NMR Restraints Lift Residual Dipolar Coupling Degeneracy in Multidomain Detergent-Solubilized Membrane Proteins. Paramagnetic-Based NMR Restraints Lift Residual Dipolar Coupling Degeneracy in Multidomain Detergent-Solubilized Membrane Proteins. J Am Chem Soc. 2011 Feb 2; Authors: Shi L, Traaseth NJ, Verardi R, Gustavsson M, Gao J, Veglia G Residual dipolar couplings (RDCs) are widely used as orientation-dependent NMR restraints to improve the resolution of the NMR conformational ensemble of biomacromolecules and define the...	nmrlearner	Journal club	0	02-04-2011 11:34 AM
Paramagnetic-Based NMR Restraints Lift Residual Dipolar Coupling Degeneracy in Multidomain Detergent-Solubilized Membrane Proteins Paramagnetic-Based NMR Restraints Lift Residual Dipolar Coupling Degeneracy in Multidomain Detergent-Solubilized Membrane Proteins Lei Shi, Nathaniel J. Traaseth, Raffaello Verardi, Martin Gustavsson, Jiali Gao and Gianluigi Veglia http://pubs.acs.org/appl/literatum/publisher/achs/journals/content/jacsat/0/jacsat.ahead-of-print/ja109080t/aop/images/medium/ja-2010-09080t_0003.gif Journal of the American Chemical Society DOI: 10.1021/ja109080t http://feeds.feedburner.com/~ff/acs/jacsat?d=yIl2AUoC8zA http://feeds.feedburner.com/~r/acs/jacsat/~4/Tu9H79dfKCk	nmrlearner	Journal club	0	02-03-2011 06:45 AM
[NMR paper] Detecting protein kinase recognition modes of calmodulin by residual dipolar coupling Detecting protein kinase recognition modes of calmodulin by residual dipolar couplings in solution NMR. Related Articles Detecting protein kinase recognition modes of calmodulin by residual dipolar couplings in solution NMR. Biochemistry. 2002 Oct 29;41(43):12899-906 Authors: Mal TK, Skrynnikov NR, Yap KL, Kay LE, Ikura M Calmodulin-regulated serine/threonine kinases (CaM kinases) play crucial roles in Ca2+-dependent signaling transduction pathways in eukaryotes. Despite having a similar overall molecular architecture of catalytic and...	nmrlearner	Journal club	0	11-24-2010 08:58 PM

« Previous Thread | Next Thread »

Posting Rules
You may not post new threads You may not post replies You may not post attachments You may not edit your posts BB code is On Smilies are On [IMG] code is On HTML code is On Trackbacks are Off Pingbacks are Off Refbacks are Off