Abstract
We structurally compare, using solid-state NMR, two different polymorphs of ?-synuclein which, as established recently, display contrasting biochemical properties, toxicity, and tropism for cells. We show that both forms, which can each be produced as a pure polymorph, are greatly different in secondary structure. While ?-sheets are the dominating secondary structure elements for both polymorphs, they are markedly divergent in terms of number of elements, as well as their distribution. We demonstrate that all identified ?-sheets feature an in-register parallel stacking for both polymorphs. The two forms show a different molecular arrangement in the unit cell and distinct dynamic features, while sharing a highly flexible C-terminal domain. The use of reproducible, well-identified conditions for sample preparation and the recording of identical NMR experiments allows for a direct comparison of the results.
[NMR images] unlike proton nmr spectroscopy the relative strength of carbon nmr
http://www2.chemistry.msu.edu/faculty/reusch/VirtTxtJml/Spectrpy/nmr/Images/ccamphor.gif
3/06/2014 10:43:59 AM GMT
unlike proton nmr spectroscopy the relative strength of carbon nmr
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nmrlearner
NMR pictures
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06-03-2014 10:43 AM
[NMR paper] Temperature-dependent structural changes of Parkinson's alpha-synuclein reveal the role of pre-existing oligomers in alpha-synuclein fibrillization.
Temperature-dependent structural changes of Parkinson's alpha-synuclein reveal the role of pre-existing oligomers in alpha-synuclein fibrillization.
http://www.ncbi.nlm.nih.gov/corehtml/query/egifs/http:--www.plosone.org-images-pone_120x30.png http://www.ncbi.nlm.nih.gov/corehtml/query/egifs/http:--www.pubmedcentral.nih.gov-corehtml-pmc-pmcgifs-pubmed-pmc.gif Related Articles Temperature-dependent structural changes of Parkinson's alpha-synuclein reveal the role of pre-existing oligomers in alpha-synuclein fibrillization.
PLoS One. 2013;8(1):e53487
Authors: ...
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Journal club
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02-03-2013 10:19 AM
[NMR paper] Expanding the Repertoire of Amyloid Polymorphs by Co-polymerization of Related Protein Precursors.
Expanding the Repertoire of Amyloid Polymorphs by Co-polymerization of Related Protein Precursors.
Related Articles Expanding the Repertoire of Amyloid Polymorphs by Co-polymerization of Related Protein Precursors.
J Biol Chem. 2013 Jan 17;
Authors: Sarell CJ, Woods LA, Su Y, Debelouchina GT, Ashcroft AE, Griffin RG, Stockley PG, Radford SE
Abstract
Amyloid fibrils can be generated from proteins with diverse sequences and folds. While amyloid fibrils assembled in vitro commonly involve a single protein precursor, fibrils formed in vivo can...
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02-03-2013 10:19 AM
Cell contents may be key to controlling toxicity of Huntington's protein - R & D Magazine
Cell contents may be key to controlling toxicity of Huntington's protein - R & D Magazine
<img alt="" height="1" width="1" />
Cell contents may be key to controlling toxicity of Huntington's protein
R & D Magazine
... suggests that the toxic effects of the huntingtin protein on cells may not be driven ... of a new 0.75-mm solid state nuclear magnetic resonance (NMR) probe.
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06-13-2012 11:34 AM
NMR determination of pK(a) values in ?-synuclein.
NMR determination of pK(a) values in ?-synuclein.
NMR determination of pK(a) values in ?-synuclein.
Protein Sci. 2011 Feb;20(2):256-69
Authors: Croke RL, Patil SM, Quevreaux J, Kendall DA, Alexandrescu AT
The intrinsically unfolded protein ?-synuclein has an N-terminal domain with seven imperfect KTKEGV sequence repeats and a C-terminal domain with a large proportion of acidic residues. We characterized pK(a) values for all 26 sites in the protein that ionize below pH 7 using 2D (1) H-(15) N HSQC and 3D C(CO)NH NMR experiments. The N-terminal...
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02-02-2011 02:40 AM
Study of acute biochemical effects of thallium toxicity in mouse urine by NMR spectroscopy.
Study of acute biochemical effects of thallium toxicity in mouse urine by NMR spectroscopy.
Study of acute biochemical effects of thallium toxicity in mouse urine by NMR spectroscopy.
J Appl Toxicol. 2011 Jan 7;
Authors: Tyagi R, Rana P, Khan AR, Bhatnagar D, Devi MM, Chaturvedi S, Tripathi RP, Khushu S
Thallium (Tl) is a toxic heavy metal and its exposure to the human body causes physiological and biochemical changes due to its interference with potassium-dependent biological reactions. A high-resolution (1) H NMR spectroscopy based metabonomic...
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01-11-2011 11:27 PM
[NMR900 blog] Opportunities for studying polymorphs and cement-based materials via Ca-43 solid-stat
Opportunities for studying polymorphs and cement-based materials via Ca-43 solid-state NMR
June 11, 2010, University of Ottawa
Calcium is an important component in diverse materials and biochemicals. However, NMR spectroscopy of the only spin-active calcium isotope, Ca-43, is notoriously challenging due to its low natural abundance (0.14 %), low resonance frequency, and quadrupolar nature. Recently, researchers from the University of Ottawa, the NRC Steacie Institute for Molecular Sciences (SIMS-NRC), and Dalhousie University have independently reported advances in studies of inorganic...
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08-22-2010 02:30 AM
[NMR900 blog] Opportunities for studying polymorphs and cement-based materials via Ca-43 solid-stat
Opportunities for studying polymorphs and cement-based materials via Ca-43 solid-state NMR
June 11, 2010, University of Ottawa
Calcium is an important component in diverse materials and biochemicals. However, NMR spectroscopy of the only spin-active calcium isotope, Ca-43, is notoriously challenging due to its low natural abundance (0.14 %), low resonance frequency, and quadrupolar nature. Recently, researchers from the University of Ottawa, the NRC Steacie Institute for Molecular Sciences (SIMS-NRC), and Dalhousie University have independently reported advances in studies of inorganic...
Unlike twins: an NMR comparison of two ?-synuclein polymorphs featuring different toxicity.
Linear Mode
