CRISPR-Cas9 is a widely utilized biochemical tool with applications in biotechnology and in medicine. Target DNA recognition and nuclease sites of Cas9 are spatially separated yet functionally coupled, indicative of an allosteric crosstalk throughout the enzyme. There are structural and dynamic changes occuring throughout Cas9 that are critical for this signal propagation. However, an understanding of the molecular motions guiding Cas9 and how this influences its mechanism are unknown. In this...
[NMR paper] Spotlight on the Ballet of Proteins: The Structural Dynamic Properties of Proteins Illuminated by Solution NMR.
Spotlight on the Ballet of Proteins: The Structural Dynamic Properties of Proteins Illuminated by Solution NMR.
Related Articles Spotlight on the Ballet of Proteins: The Structural Dynamic Properties of Proteins Illuminated by Solution NMR.
Int J Mol Sci. 2020 Mar 06;21(5):
Authors: Tokunaga Y, Viennet T, Arthanari H, Takeuchi K
Abstract
Solution NMR spectroscopy is a unique and powerful technique that has the ability to directly connect the structural dynamics of proteins in physiological conditions to their activity and...
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03-16-2020 04:59 PM
Unique Insights into the Structural and Functional Biology of Membrane Proteins from Solid State NMR Spectroscopy
Unique Insights into the Structural and Functional Biology of Membrane Proteins from Solid State NMR Spectroscopy
Publication date: 2 February 2018
Source:Biophysical Journal, Volume 114, Issue 3, Supplement 1</br>
Author(s): Timothy Cross, Joana Paulino, Huajun Qin, Yiseul Shin, Cristian Escobar, Rongfu Zhang, Joshua Taylor, Yimin Miao, Riqiang Fu, Eduard Chekmenev, Ivan Hung, Zhehong Gan, Petr Gor'kov</br>
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02-07-2018 03:41 PM
[NMR paper] Just a Flexible Linker? The Structural and Dynamic Properties of CBP-ID4 Revealed by NMR Spectroscopy.
Just a Flexible Linker? The Structural and Dynamic Properties of CBP-ID4 Revealed by NMR Spectroscopy.
Just a Flexible Linker? The Structural and Dynamic Properties of CBP-ID4 Revealed by NMR Spectroscopy.
Biophys J. 2016 Jan 19;110(2):372-381
Authors: Piai A, Calçada EO, Tarenzi T, Grande AD, Varadi M, Tompa P, Felli IC, Pierattelli R
Abstract
Here, we present a structural and dynamic description of CBP-ID4 at atomic resolution. ID4 is the fourth intrinsically disordered linker of CREB-binding protein (CBP). In spite of the...
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01-21-2016 01:08 PM
[NMR paper] A Unique Tool for Cellular Structural Biology: In-cell NMR.
A Unique Tool for Cellular Structural Biology: In-cell NMR.
Related Articles A Unique Tool for Cellular Structural Biology: In-cell NMR.
J Biol Chem. 2015 Dec 16;
Authors: Luchinat E, Banci L
Abstract
Conventional structural and chemical biology approaches are applied to macromolecules extrapolated from their native context. When doing so, important structural and functional features of macromolecules may be lost, which depend on their native network of interactions within the cell. In-cell nuclear magnetic resonance is a branch...
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[NMR paper] Unique Structure and Dynamics of the EphA5 Ligand Binding Domain Mediate Its Binding Specificity as Revealed by X-ray Crystallography, NMR and MD Simulations.
Unique Structure and Dynamics of the EphA5 Ligand Binding Domain Mediate Its Binding Specificity as Revealed by X-ray Crystallography, NMR and MD Simulations.
Unique Structure and Dynamics of the EphA5 Ligand Binding Domain Mediate Its Binding Specificity as Revealed by X-ray Crystallography, NMR and MD Simulations.
PLoS One. 2013;8(9):e74040
Authors: Huan X, Shi J, Lim L, Mitra S, Zhu W, Qin H, Pasquale EB, Song J
Abstract
The 16 EphA and EphB receptors represent the largest family of receptor tyrosine kinases, and their interactions...
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10-03-2013 03:31 PM
[NMR paper] Structural and dynamic properties of the Fv fragment and the single-chain Fv fragment
Structural and dynamic properties of the Fv fragment and the single-chain Fv fragment of an antibody in solution investigated by heteronuclear three-dimensional NMR spectroscopy.
Related Articles Structural and dynamic properties of the Fv fragment and the single-chain Fv fragment of an antibody in solution investigated by heteronuclear three-dimensional NMR spectroscopy.
Biochemistry. 1994 Mar 22;33(11):3296-303
Authors: Freund C, Ross A, Plückthun A, Holak TA
Fv fragments, heterodimers of the variable light (VL) and variable heavy chain...
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[NMR paper] Structural and dynamic properties of the Fv fragment and the single-chain Fv fragment
Structural and dynamic properties of the Fv fragment and the single-chain Fv fragment of an antibody in solution investigated by heteronuclear three-dimensional NMR spectroscopy.
Related Articles Structural and dynamic properties of the Fv fragment and the single-chain Fv fragment of an antibody in solution investigated by heteronuclear three-dimensional NMR spectroscopy.
Biochemistry. 1994 Mar 22;33(11):3296-303
Authors: Freund C, Ross A, Plückthun A, Holak TA
Fv fragments, heterodimers of the variable light (VL) and variable heavy chain...
Unique structural and dynamic properties of the HNH nuclease in mesophilic and thermophilic Cas9 revealed by NMR
Linear Mode
