The 3D HCCH-TOCSY and HCC(CO)NH-TOCSY experiments provide through bond connectivity and are used for side-chain chemical shift assignment by solution-state NMR. Careful design and implementation of the pulse sequence are key to the successful application of the technique particularly when trying to extract the maximum information out of challenging biomolecules. Here we investigate the source of and propose solutions for abnormal peak splitting ranging from 152 to 80Â*Hz and below that were found in three popular TOCSY-based experiment types: H(F1)â??C(F2)â??DIPSIâ??H(F3), C(F1)â??DIPSIâ??C(F2)â??H(F3), and C(F1)â??DIPSIâ??N(F2)â??HN(F3). Peak splitting occurs in the indirect C(F1) or C(F2) dimension before DIPSI and analyses indicate that the artifacts are resulted mainly from the DIPSI transforming a double spin order \(2{C}_{1y}{C}_{2x}\) from 13Câ??13C scalar 1JCC coupling during t1 into observable megnetization. The splitting is recapitulated by numerical simulation and approaches are proposed to remove it. Adding a pure delay of 3.7Â*ms immediately before DIPSI is a simple and effective strategy to achieve 3D HCCH-TOCSY and HCC(CO)NH-TOCSY spectra free of splitting with full crosspeak intensity.
[Question from NMRWiki Q&A forum] Calibrate HCCH-TOCSY spectrum with reference to HBHACONH
Calibrate HCCH-TOCSY spectrum with reference to HBHACONH
I am facing problems in calibration of my HCCH-TOCSY spectrum in reference to HBHACONH and HCCCONH spectrum. Can somebody tell me how to solve this issue? Can I autoassign my HCCH-TOCSY spectrum using HBHACONH spectrum
Check if somebody has answered this question on NMRWiki QA forum
nmrlearner
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10-02-2011 08:25 AM
[NMRpipe Yahoo group] Re: N15 separated NOESY and TOCSY
Re: N15 separated NOESY and TOCSY
Hi Iris, Another question is what do you mean by the NH vs HH planes in regards to the 3D matrix (a single 3D peak can't be positive viewed along one axis and
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05-27-2011 10:40 AM
[NMRpipe Yahoo group] N15 separated NOESY and TOCSY
N15 separated NOESY and TOCSY
Hello, I did a N15 separated TOCSY experiment and used the following script: #!/bin/csh bruk2pipe -in ./ser \ -bad 0.0 -aswap -DMX -decim 2632 -dspfvs 20
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05-26-2011 10:34 PM
[NMRwiki tweet] nmrwiki: Why HCCH TOCSY does not work for whole aromatic side chains? #nmrhttp://qa.n
nmrwiki: Why HCCH TOCSY does not work for whole aromatic side chains? #nmrhttp://qa.nmrwiki.org/question/199/
nmrwiki: Why HCCH TOCSY does not work for whole aromatic side chains? #nmrhttp://qa.nmrwiki.org/question/199/
Source: NMRWiki tweets
nmrlearner
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11-18-2010 06:16 PM
[Question from NMRWiki Q&A forum] HCCH-Tocsy connectivities
HCCH-Tocsy connectivities
What I see from this picture (linked from www.protein-nmr.org.uk):
http://www.protein-nmr.org.uk/pictures/experiment_types/hcch_tocsy.png
that shows an HCCH-TOCSY magnetisation transfer I do not see any transfer from HD to HB, but in my peaks I see a peak that seems it can be connected az Leu CB-HB2-HD22Can I have these peaks in HCCH-TOCSY?
would you please introduce me a reference that tell me what kind of connection can be visible in HC(C)H-Tocsy
nmrlearner
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11-17-2010 06:08 PM
[Nature network NMR forum] HCCH-TOCSY (0 replies)
HCCH-TOCSY (0 replies)
Hello everybody,
I am a biologist who has jumped into NMR :(
I hope this is an active forum so that I can improve myself and can help someday to others.
I have a very stupid question!!! Do you know if I can see some HN peaks correlated with C-beta in HCCH-TOCSY?
Cheers,
nmrlearner
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08-25-2010 02:58 AM
[U. of Ottawa NMR Facility Blog] Hsqc - tocsy
HSQC - TOCSY
There are many different NMR techniques used to probe molecular structure. Two very useful methods are the HSQC (or HMQC) sequence used to obtain heteronuclear coupling correlations and the TOCSY sequence used to look at extended homonuclear coupling correlations. These are among the most widely used pulse sequences by chemists to help elucidate the molecular structure of organic molecules. The sequences can also be combined into a single HSQC-TOCSY sequence which is simply an HSQC followed by a TOCSY spin lock. For the case of 1H and 13C in organic molecules, the HSQC-TOCSY...
Understanding and solving abnormal peak splitting in 3D HCCH-TOCSY and HCC(CO)NH-TOCSY
Linear Mode
