Related ArticlesUnderstanding Molecular Mechanism of Biologics Drug Delivery and Stability from NMR Spectroscopy.
Adv Drug Deliv Rev. 2021 Feb 17;:
Authors: Phyo P, Zhao X, Templeton AC, Xu W, Cheung JK, Su Y
Abstract
Protein therapeutics carry inherent limitations of membrane impermeability and structural instability, despite their predominant role in the modern pharmaceutical market. Effective formulations are needed to overcome physiological and physicochemical barriers, respectively, for improving bioavailability and stability. Knowledge of membrane affinity, cellular internalization, encapsulation, and release of drug-loaded carrier vehicles uncover the structural basis for designing and optimizing biopharmaceuticals with enhanced delivery efficiency and therapeutic efficacy. Understanding stabilizing and destabilizing interactions between protein drugs and formulation excipients provide fundamental mechanisms for ensuring the stability and quality of biological products. This article reviews the molecular studies of biologics using solution and solid-state NMR spectroscopy on structural attributes pivotal to drug delivery and stability. In-depth investigation of the structure-function relationship of drug delivery systems based on cell-penetrating peptides, lipid nanoparticles and polymeric colloidal, and biophysical and biochemical stability of peptide, protein, monoclonal antibody, and vaccine, as the integrative efforts on drug product design, will be elaborated.
PMID: 33609600 [PubMed - as supplied by publisher]
[NMR paper] Noninvasive characterization (EPR, ?CT, NMR) of 3D PLA electrospun fiber sponges for controlled drug delivery.
Noninvasive characterization (EPR, ?CT, NMR) of 3D PLA electrospun fiber sponges for controlled drug delivery.
Related Articles Noninvasive characterization (EPR, ?CT, NMR) of 3D PLA electrospun fiber sponges for controlled drug delivery.
Int J Pharm X. 2020 Dec;2:100055
Authors: Zech J, Mader M, Gündel D, Metz H, Odparlik A, Agarwal S, Mäder K, Greiner A
Abstract
Highly porous 3D-scaffolds, made from cut, electrospun PLA fibers, are relatively new and promising systems for controlled drug-delivery applications. Because knowledge...
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09-29-2020 07:53 PM
[NMR paper] The contribution of solid-state NMR spectroscopy to understanding biomineralization: Atomic and molecular structure of bone.
The contribution of solid-state NMR spectroscopy to understanding biomineralization: Atomic and molecular structure of bone.
http://www.bionmr.com//www.ncbi.nlm.nih.gov/corehtml/query/egifs/http:--linkinghub.elsevier.com-ihub-images-PubMedLink.gif The contribution of solid-state NMR spectroscopy to understanding biomineralization: Atomic and molecular structure of bone.
J Magn Reson. 2015 Apr;253:98-110
Authors: Duer MJ
Abstract
Solid-state NMR spectroscopy has had a major impact on our understanding of the structure of...
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[NMR paper] The contribution of solid-state NMR spectroscopy to understanding biomineralization: Atomic and molecular structure of bone
The contribution of solid-state NMR spectroscopy to understanding biomineralization: Atomic and molecular structure of bone
Publication date: April 2015
Source:Journal of Magnetic Resonance, Volume 253</br>
Author(s): Melinda J. Duer</br>
Solid-state NMR spectroscopy has had a major impact on our understanding of the structure of mineralized tissues, in particular bone. Bone exemplifies the organic–inorganic composite structure inherent in mineralized tissues. The organic component of the extracellular matrix in bone is primarily composed of ordered fibrils of...
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[NMR paper] Understanding the mechanism of prosegment-catalyzed folding by solution NMR spectroscopy.
Understanding the mechanism of prosegment-catalyzed folding by solution NMR spectroscopy.
Related Articles Understanding the mechanism of prosegment-catalyzed folding by solution NMR spectroscopy.
J Biol Chem. 2013 Nov 21;
Authors: Wang S, Horimoto Y, Dee DR, Yada RY
Abstract
Multidomain protein folding is often more complex than a two-state process which leads to the spontaneous folding of the native state. Pepsin, a zymogen-derived enzyme, without its prosegment (PS), is irreversibly denatured and folds to a thermodynamically stable,...
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11-23-2013 05:08 PM
NMR techniques in drug delivery: Application to zein protein complexes
NMR techniques in drug delivery: Application to zein protein complexes
15 December 2012
Publication year: 2012
Source:International Journal of Pharmaceutics, Volume 439, Issues 1–2</br>
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Zein is a protein containing a large amount of nonpolar amino acids, which has shown the ability to form aggregates and entrap solutes, such as drugs and amino acids. NMR techniques were used to detect binding interactions and measure affinity between zein and three different drugs: tetracycline, amoxicillin and indomethacin. The release study of zein microparticle formulations...
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02-03-2013 10:13 AM
Understanding the radical mechanism of lipoxygenases using 31P NMR spin trapping.
Understanding the radical mechanism of lipoxygenases using 31P NMR spin trapping.
Understanding the radical mechanism of lipoxygenases using 31P NMR spin trapping.
Bioorg Med Chem. 2011 May 1;19(9):3022-8
Authors: Zoia L, Perazzini R, Crestini C, Argyropoulos DS
Abstract
In this paper, we use our quantitative (31)P NMR spin trapping methods, already developed for simple oxygen- and carbon-centered radicals, to understand the radical intermediates generated by enzymatic systems and more specifically lipoxygenases. Our methodology...
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08-25-2011 04:10 PM
Four sessions to unveil groundbreaking cellular analysis tools, drug-delivery ... - Newswise (press release)
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Four sessions to unveil groundbreaking cellular analysis tools, drug-delivery ...
Newswise (press release)
â??The high-resolution protein structures that you see on Wikipedia have been obtained by X-ray crystallography and nuclear magnetic resonance -- and these are important reference points for understanding proteins in biology,â?? explains Michael L. Gross. ...
Four sessions to unveil groundbreaking cellular analysis tools, drug-delivery ... - Newswise (press release)
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04-07-2011 11:15 AM
[NMR paper] Stability of beta-galactosidase, a model protein drug, is related to water mobility a
Stability of beta-galactosidase, a model protein drug, is related to water mobility as measured by 17O nuclear magnetic resonance (NMR).
Related Articles Stability of beta-galactosidase, a model protein drug, is related to water mobility as measured by 17O nuclear magnetic resonance (NMR).
Pharm Res. 1993 Jan;10(1):103-8
Authors: Yoshioka S, Aso Y, Izutsu K, Terao T
The inactivation of freeze-dried beta-galactosidase during storage was studied, focusing on the effect of water mobility as measured by the spin-lattice relaxation time, T1, of...
Understanding Molecular Mechanism of Biologics Drug Delivery and Stability from NMR Spectroscopy.
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