[NMR paper] Unambiguous identification of ?-Gal epitopes in intact monoclonal antibodies by NMR spectroscopy
Unambiguous identification of ?-Gal epitopes in intact monoclonal antibodies by NMR spectroscopy
The ?-Gal epitope consisting of the terminal trisaccharide Gal?1,3Gal?1,4GlcNAc exposed on cell or protein surfaces can cause severe immune reactions, such as hypersensitivity reactions, in humans. This epitope is also called the xenotransplantation epitope because it is one of the main reasons for the rejection of non-human organ transplants by the human innate immune response. Recombinant therapeutic proteins expressed in murine cell lines may contain ?-Gal epitopes, and therefore their...
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10-16-2022 03:12 AM
[NMR paper] Ligand-induced structural transitions combined with paramagnetic ions facilitate unambiguous NMR assignments of methyl groups in large proteins
Ligand-induced structural transitions combined with paramagnetic ions facilitate unambiguous NMR assignments of methyl groups in large proteins
NMR spectroscopy allows the study of biomolecules in close-to-native conditions. Structural information can be inferred from the NMR spectra when an assignment is available. Protein assignment is usually a time-consuming task, being specially challenging in the case of large, supramolecular systems. Here, we present an extension of existing state-of-the-art strategies for methyl group assignment that partially overcomes signal overlapping and other...
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04-11-2022 10:19 AM
Ligand-induced structural transitions combined with paramagnetic ions facilitate unambiguous NMR assignments of methyl groups in large proteins
Ligand-induced structural transitions combined with paramagnetic ions facilitate unambiguous NMR assignments of methyl groups in large proteins
Abstract
NMR spectroscopy allows the study of biomolecules in close-to-native conditions. Structural information can be inferred from the NMR spectra when an assignment is available. Protein assignment is usually a time-consuming task, being specially challenging in the case of large, supramolecular systems. Here, we present an extension of existing state-of-the-art strategies for methyl group assignment that...
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04-10-2022 09:31 PM
[ASAP] Staphylococcus aureus Glucose-Induced Biofilm Accessory Protein A (GbaA) Is a Monothiol-Dependent Electrophile Sensor
Staphylococcus aureus Glucose-Induced Biofilm Accessory Protein A (GbaA) Is a Monothiol-Dependent Electrophile Sensor
https://pubs.acs.org/na101/home/literatum/publisher/achs/journals/content/bichaw/0/bichaw.ahead-of-print/acs.biochem.0c00347/20200729/images/medium/bi0c00347_0008.gif
Biochemistry
DOI: 10.1021/acs.biochem.0c00347
http://feeds.feedburner.com/~r/acs/bichaw/~4/aVvLgIVQo9U
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[NMR paper] A new glycation product 'norpronyl-lysine,' and direct characterization of cross linking and other glycation adducts: NMR of model compounds and collagen.
A new glycation product 'norpronyl-lysine,' and direct characterization of cross linking and other glycation adducts: NMR of model compounds and collagen.
Related Articles A new glycation product 'norpronyl-lysine,' and direct characterization of cross linking and other glycation adducts: NMR of model compounds and collagen.
Biosci Rep. 2014 Apr 01;34(2):
Authors: Bullock PT, Reid DG, Ying Chow W, Lau WP, Duer MJ
Abstract
NMR is ideal for characterizing non-enzymatic protein glycation, including AGEs (advanced glycation...
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[NMR paper] A new glycation product 'norpronyl-lysine' and direct characterization of cross linking and other glycation adducts: NMR of model compounds and collagen.
A new glycation product 'norpronyl-lysine' and direct characterization of cross linking and other glycation adducts: NMR of model compounds and collagen.
Related Articles A new glycation product 'norpronyl-lysine' and direct characterization of cross linking and other glycation adducts: NMR of model compounds and collagen.
Biosci Rep. 2014 Feb 12;
Authors: Bullock PT, Reid DG, Chow WY, Lau WP, Duer MJ
Abstract
NMR is ideal for characterizing non-enzymatic protein glycation, including AGEs (advanced glycation end products) underlying...
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02-13-2014 03:35 PM
[NMR paper] Reduced Dimensionality tailored HN(C)N Experiments for Facile Backbone Resonance Assignment of Proteins through Unambiguous Identification of Sequential HSQC Peaks
Reduced Dimensionality tailored HN(C)N Experiments for Facile Backbone Resonance Assignment of Proteins through Unambiguous Identification of Sequential HSQC Peaks
Publication date: Available online 8 October 2013
Source:Journal of Magnetic Resonance</br>
Author(s): Dinesh Kumar</br>
Two novel reduced dimensionality (RD) tailored HN(C)N experiments are proposed to facilitate the backbone resonance assignment of proteins both in terms of its accuracy and speed. These experiments -referred here as (4,3)D-hNCOcaNH and (4,3)D-hNcoCANH- exploit the linear combination of...
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10-09-2013 05:31 AM
[NMR paper] Detection of modifications in the glucose metabolism induced by genetic mutations in
Detection of modifications in the glucose metabolism induced by genetic mutations in Saccharomyces cerevisiae by 13C- and H-NMR spectroscopy.
Related Articles Detection of modifications in the glucose metabolism induced by genetic mutations in Saccharomyces cerevisiae by 13C- and H-NMR spectroscopy.
Eur J Biochem. 2000 Jun;267(11):3337-44
Authors: Herve M, Buffin-Meyer B, Bouet F, Son TD
NMR spectroscopy may offer a suitable technique to characterize the glucose metabolism in response to genetic mutations in cells. The effects of various...
Unambiguous Identification of Glucose-Induced Glycation in mAbs and other Proteins by NMR Spectroscopy
Linear Mode
