NMR paper Ultrashort Broadband Cooperative Pulses for Multidimensional Biomolecular NMR Experiments.

		BioNMR > Educational resources > Journal club
[NMR paper] Ultrashort Broadband Cooperative Pulses for Multidimensional Biomolecular NMR Experiments.

Webservers

NMR processing:

MDD

NMR assignment:

Backbone:

Side-chains:

NOEs:

Structure from NMR restraints:

Ab initio:

Fragment-based:

Rosetta-NMR (Robetta)

Template-based:

GeNMR

I-TASSER

Refinement:

Amber

Structure from chemical shifts:

Fragment-based:

Homology-based:

Torsion angles from chemical shifts:

Preditor

TALOS

Promega- Proline

Secondary structure from chemical shifts:

CSI (via RCI server)

TALOS

MICS caps, β-turns

d2D

PECAN

Flexibility from chemical shifts:

RCI

Interactions from chemical shifts:

HADDOCK

Chemical shifts re-referencing:

NMR model quality:

NOEs, other restraints:

Chemical shifts:

RDCs:

Pseudocontact shifts:

Protein geomtery:

SAVES2 or SAVES4

Quality Control Check

NMR spectrum prediction:

FANDAS

MestReS

V-NMR

Flexibility from structure:

Backbone S2

Methyl S2

B-factor

Molecular dynamics:

Gromacs

Amber

Antechamber

Chemical shifts prediction:

From structure:

Shiftx2

Sparta+

Camshift

CH3shift- Methyl

ArShift- Aromatic

ShiftS

Proshift

PPM

CheShift-2- Cα

From sequence:

Shifty

Camcoil

Poulsen_rc_CS

Disordered proteins:

MAXOCC

Format conversion & validation:

CCPN

From NMR-STAR 3.1

Validate NMR-STAR 3.1

NMR sample preparation:

Protein disorder:

DisMeta

Protein solubility:

Isotope labeling:

Solid-state NMR:

Thread Tools

Search this Thread

Rate Thread

Display Modes

03-07-2018, 08:27 PM

nmrlearner

Senior Member

Join Date: Jan 2005

Posts: 23,777

Points: 193,617, Level: 100

Level up: 0%, 0 Points needed

Activity: 50.7%

Last Achievements

Award-Showcase

NMR Credits: 0

NMR Points: 193,617

Downloads: 0

Uploads: 0

Ultrashort Broadband Cooperative Pulses for Multidimensional Biomolecular NMR Experiments.

Ultrashort Broadband Cooperative Pulses for Multidimensional Biomolecular NMR Experiments.

Related Articles Ultrashort Broadband Cooperative Pulses for Multidimensional Biomolecular NMR Experiments.

Angew Chem Int Ed Engl. 2018 Mar 05;:

Authors: Asami S, Kallies W, Günther J, Stavropoulou M, Glaser S, Sattler M

Abstract
NMR spectroscopy at ultra-high magnetic fields requires improved radiofrequency (rf) pulses to cover the increased spectral bandwidth. We introduce optimized 90° pulse pairs as Ramsey-type cooperative (Ram-COOP) pulses for biomolecular NMR applications. The Ram-COOP element provides broadband excitation with enhanced sensitivity and reduced artifacts even at magnetic fields >1.0 GHz 1H Larmor frequency (23 T). A pair of 30 ?s Ram-COOP pulses achieves an excitation bandwidth of 100 kHz with a maximum rf field of 20 kHz, more than three-fold improved compared to achievable excitation by rectangular pulses. Ram-COOP pulses exhibit little offset-dependent phase errors and are robust to rf inhomogeneity. The performance of the Ram-COOP element is experimentally confirmed with heteronuclear multidimensional NMR experiments, applied to proteins and nucleic acids. Ram-COOP provides broadband excitation at low rf field strength suitable for application at current magnetic fields and beyond 23 T.

PMID: 29508496 [PubMed - as supplied by publisher]

More...

Did you find this post helpful?

Similar Threads
Thread	Thread Starter	Forum	Replies	Last Post
Enhancing the sensitivity of multidimensional NMR experiments by using triply-compensated Ï? pulses Enhancing the sensitivity of multidimensional NMR experiments by using triply-compensated Ï? pulses Abstract In multidimensional solution NMR experiments, Ï? pulses are used extensively for inversion and refocusing operations on 1H, 13C and 15N nuclei. Pulse miscalibration, off-resonance effects, and J-coupling evolution during Ï? pulse execution result in severe signal losses that are exacerbated at high magnetic fields. Here, we report the implementation of a triply-compensated Ï? pulse (G5) optimized for both inversion and refocusing in widely used...	nmrlearner	Journal club	0	11-21-2017 10:10 PM
[NMR paper] Applications of high dimensionality experiments to biomolecular NMR. Applications of high dimensionality experiments to biomolecular NMR. Applications of high dimensionality experiments to biomolecular NMR. Prog Nucl Magn Reson Spectrosc. 2015 Nov;90-91:49-73 Authors: Nowakowski M, Saxena S, Stanek J, ?erko S, Ko?mi?ski W Abstract High dimensionality NMR experiments facilitate resonance assignment and precise determination of spectral parameters such as coupling constants. Sparse non-uniform sampling enables acquisition of experiments of high dimensionality with high resolution in acceptable...	nmrlearner	Journal club	0	11-26-2015 12:13 AM
Applications of high dimensionality experiments to biomolecular NMR Applications of high dimensionality experiments to biomolecular NMR Publication date: Available online 11 July 2015 Source:Progress in Nuclear Magnetic Resonance Spectroscopy</br> Author(s): Micha? Nowakowski , Saurabh Saxena , Jan Stanek , Szymon ?erko , Wiktor Ko?mi?ski</br> High dimensionality NMR experiments facilitate resonance assignment and precise determination of spectral parameters such as coupling constants. Sparse non-uniform sampling enables acquisition of experiments of high dimensionality with high resolution in acceptable time. In this review...	nmrlearner	Journal club	0	07-12-2015 07:12 AM
Optimization of an absolute sensitivity in a glassy matrix during DNP-enhanced multidimensional solid-state NMR experiments From The DNP-NMR Blog: Optimization of an absolute sensitivity in a glassy matrix during DNP-enhanced multidimensional solid-state NMR experiments Takahashi, H., et al., Optimization of an absolute sensitivity in a glassy matrix during DNP-enhanced multidimensional solid-state NMR experiments. J Magn Reson, 2013. 239C(0): p. 91-99. http://www.ncbi.nlm.nih.gov/pubmed/24480716	nmrlearner	News from NMR blogs	0	02-10-2014 08:46 PM
Simultaneous convection compensation and solvent suppression in biomolecular NMR diffusion experiments Simultaneous convection compensation and solvent suppression in biomolecular NMR diffusion experiments Abstract Thermal convection and high intensity solvent resonances can significantly hamper diffusion estimates in pulsed gradient spin-echo nuclear magnetic resonance diffusion experiments on biomolecule samples. To overcome these two problems, a new double functional NMR diffusion sequence, double echo PGSTE-WATERGATE, is presented. The new sequence provides excellent convection compensation and solvent suppression (with a suppression factor in excess of at least 105 in a single scan)...	nmrlearner	Journal club	0	01-09-2011 12:46 PM
[NMR paper] Simple multidimensional NMR experiments to obtain different types of one-bond dipolar Simple multidimensional NMR experiments to obtain different types of one-bond dipolar couplings simultaneously. Related Articles Simple multidimensional NMR experiments to obtain different types of one-bond dipolar couplings simultaneously. J Biomol NMR. 2001 Jan;19(1):63-7 Authors: de Alba E, Suzuki M, Tjandra N In order to measure residual dipolar couplings, the molecule under study has to be partially oriented in the presence of the magnetic field. It has been observed that some protein samples are not stable under the conditions imposed by...	nmrlearner	Journal club	0	11-19-2010 08:32 PM
Broadband Heteronuclear Solid-State NMR Experiments by Exponentially Modulated Dipola Broadband Heteronuclear Solid-State NMR Experiments by Exponentially Modulated Dipolar Recoupling without Decoupling. http://www.ncbi.nlm.nih.gov/corehtml/query/egifs/http:--www.pubmedcentral.nih.gov-corehtml-pmc-pmcgifs-pubmed-pmc-MS.gif Related Articles Broadband Heteronuclear Solid-State NMR Experiments by Exponentially Modulated Dipolar Recoupling without Decoupling. J Phys Chem Lett. 2010 Jun 1;1(13):1952-1956 Authors: Nielsen AB, StraasÃ¸ LA, Nieuwkoop AJ, Rienstra CM, Bjerring M, Nielsen NC We present a novel solid-state NMR method for...	nmrlearner	Journal club	0	08-17-2010 03:36 AM
Spatially encoded strategies in the execution of biomolecular-oriented 3D NMR experiments Spatially encoded strategies in the execution of biomolecular-oriented 3D NMR experiments Mor Mishkovsky, Maayan Gal and Lucio Frydman Journal of Biomolecular NMR; 2007; 39(4); pp 291-301 Abstract: Three-dimensional nuclear magnetic resonance (3D NMR) provides one of the foremost analytical tools available for the elucidation of biomolecular structure, function and dynamics. Executing a 3D NMR experiment generally involves scanning a series of time-domain signals S(t 3), as a function of two time variables (t 1, t 2) which need to undergo parametric incrementations throughout...	Deano	Journal club	0	08-14-2008 09:53 PM

« Previous Thread | Next Thread »

Posting Rules
You may not post new threads You may not post replies You may not post attachments You may not edit your posts BB code is On Smilies are On [IMG] code is On HTML code is On Trackbacks are Off Pingbacks are Off Refbacks are Off