[ASAP] CRISPR-Cas13-Based RNA-Interacting Protein Detection in Living Cells
CRISPR-Cas13-Based RNA-Interacting Protein Detection in Living Cells
https://pubs.acs.org/na101/home/literatum/publisher/achs/journals/content/bichaw/0/bichaw.ahead-of-print/acs.biochem.0c00281/20200429/images/medium/bi0c00281_0001.gif
Biochemistry
DOI: 10.1021/acs.biochem.0c00281
http://feeds.feedburner.com/~r/acs/bichaw/~4/NV3_snWqzIU
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nmrlearner
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04-30-2020 02:58 PM
[NMR paper] Proximity-triggered covalent stabilization of low-affinity protein complexes in vitro and in vivo
Proximity-triggered covalent stabilization of low-affinity protein complexes in vitro and in vivo
The characterization of low-affinity protein complexes is challenging due to their dynamic nature. Here we present a method to stabilize transient protein complexes in vivo by generating a covalent and conformationally flexible bridge between the interaction partners. A highly active pyrrolysyl tRNA synthetase mutant directs the incorporation of unnatural amino acids bearing bromoalkyl moieties (BrCnK) into proteins. We demonstrate for the first time that low-affinity protein complexes...
nmrlearner
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09-28-2017 07:57 PM
[NMR paper] A Genetically Encoded ?-Lactamase Reporter for Ultrasensitive (129) Xe NMR in Mammalian Cells.
A Genetically Encoded ?-Lactamase Reporter for Ultrasensitive (129) Xe NMR in Mammalian Cells.
Related Articles A Genetically Encoded ?-Lactamase Reporter for Ultrasensitive (129) Xe NMR in Mammalian Cells.
Angew Chem Int Ed Engl. 2016 Jun 15;
Authors: Wang Y, Roose BW, Palovcak EJ, Carnevale V, Dmochowski IJ
Abstract
Molecular imaging holds considerable promise for elucidating biological processes in normal physiology as well as disease states, but requires noninvasive methods for identifying analytes at sub-micromolar...
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06-16-2016 12:06 PM
[NMR paper] Programming A Molecular Relay for Ultrasensitive Biodetection through (129) Xe NMR.
Programming A Molecular Relay for Ultrasensitive Biodetection through (129) Xe NMR.
http://www.bionmr.com//www.ncbi.nlm.nih.gov/corehtml/query/egifs/http:--media.wiley.com-assets-7315-19-Wiley_FullText_120x30_orange.png http://www.bionmr.com//www.ncbi.nlm.nih.gov/corehtml/query/egifs/http:--media.wiley.com-assets-7315-19-Wiley_FullText_120x30_orange.png Related Articles Programming A Molecular Relay for Ultrasensitive Biodetection through (129) Xe NMR.
Angew Chem Int Ed Engl. 2015 Dec 21;
Authors: Wang Y, Roose BW, Philbin JP, Doman JL, Dmochowski...
nmrlearner
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12-28-2015 12:26 AM
Cucurbit[6]uril is an ultrasensitive (129)Xe NMR contrast agent
From The DNP-NMR Blog:
Cucurbituril is an ultrasensitive (129)Xe NMR contrast agent
Wang, Y. and I.J. Dmochowski, Cucurbituril is an ultrasensitive (129)Xe NMR contrast agent. Chem Commun (Camb), 2015. 51(43): p. 8982-5.
http://www.ncbi.nlm.nih.gov/pubmed/25929681
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06-11-2015 05:15 AM
Diels-Alder reactionâ??triggered bioorthogonal protein decaging in living cells - Nature.com
Diels-Alder reactionâ??triggered bioorthogonal protein decaging in living cells - Nature.com
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Diels-Alder reactionâ??triggered bioorthogonal protein decaging in living cells
Nature.com
A generally applicable strategy, however, remains elusive. Herein we describe a small moleculeâ??triggered bioorthogonal protein decaging technique that relies on the inverse electron-demand Diels-Alder reaction for eliminating a chemically caged protein ...
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nmrlearner
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11-04-2014 02:32 PM
[NMR paper] Two-dimensional NMR spectroscopy reveals cation-triggered backbone degradation in polysulfone-based anion exchange membranes.
Two-dimensional NMR spectroscopy reveals cation-triggered backbone degradation in polysulfone-based anion exchange membranes.
http://www.bionmr.com//www.ncbi.nlm.nih.gov/corehtml/query/egifs/http:--highwire.stanford.edu-icons-externalservices-pubmed-custom-pnas_full.gif Related Articles Two-dimensional NMR spectroscopy reveals cation-triggered backbone degradation in polysulfone-based anion exchange membranes.
Proc Natl Acad Sci U S A. 2013 Feb 12;110(7):2490-5
Authors: Arges CG, Ramani V
Abstract
Anion exchange membranes (AEMs) find...
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04-09-2013 06:31 PM
Detection of unrealistic molecular environments in protein structures based on expect
Abstract Understanding the relationship between protein structure and biological function is a central theme in structural biology. Advances are severely hampered by errors in experimentally determined protein structures. Detection and correction of such errors is therefore of utmost importance. Electron densities in molecular structures obey certain rules which depend on the molecular environment. Here we present and discuss a new approach that relates electron densities computed from a structural model to densities expected from prior observations on identical or closely related molecular...
Ultrasensitive Detection of Aqueous Uranyl Based on Uranyl-Triggered Protein Photocleavage
Linear Mode
