Multi-receiver solid-state NMR using polarization optimized experiments (POE) at ultrafast magic angle spinning
Multi-receiver solid-state NMR using polarization optimized experiments (POE) at ultrafast magic angle spinning
Abstract
Ultrafast magic angle spinning (MAS) technology and 1H detection have dramatically enhanced the sensitivity of solid-state NMR (ssNMR) spectroscopy of biopolymers. We previously showed that, when combined with polarization optimized experiments (POE), these advancements enable the simultaneous acquisition of multi-dimensional 1H- or 13C-detected experiments using a single receiver. Here, we propose a new sub-class within the POE...
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04-25-2020 12:56 PM
[NMR paper] Proton-Based Ultrafast Magic Angle Spinning Solid-State NMR Spectroscopy.
Proton-Based Ultrafast Magic Angle Spinning Solid-State NMR Spectroscopy.
Related Articles Proton-Based Ultrafast Magic Angle Spinning Solid-State NMR Spectroscopy.
Acc Chem Res. 2017 Mar 29;:
Authors: Zhang R, Mroue KH, Ramamoorthy A
Abstract
Protons are vastly abundant in a wide range of exciting macromolecules and thus can be a powerful probe to investigate the structure and dynamics at atomic resolution using solid-state NMR (ssNMR) spectroscopy. Unfortunately, the high signal sensitivity, afforded by the high...
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03-30-2017 06:42 PM
[NMR paper] Backbone assignment of perdeuterated proteins by solid-state NMR using proton detection and ultrafast magic-angle spinning.
Backbone assignment of perdeuterated proteins by solid-state NMR using proton detection and ultrafast magic-angle spinning.
Related Articles Backbone assignment of perdeuterated proteins by solid-state NMR using proton detection and ultrafast magic-angle spinning.
Nat Protoc. 2017 Apr;12(4):764-782
Authors: Fricke P, Chevelkov V, Zinke M, Giller K, Becker S, Lange A
Abstract
Solid-state NMR (ssNMR) is a technique that allows the study of protein structure and dynamics at atomic detail. In contrast to X-ray crystallography and...
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03-10-2017 04:23 PM
[NMR paper] A Novel High-Resolution and Sensitivity-Enhanced Three-Dimensional Solid-State NMR Experiment Under Ultrafast Magic Angle Spinning Conditions.
A Novel High-Resolution and Sensitivity-Enhanced Three-Dimensional Solid-State NMR Experiment Under Ultrafast Magic Angle Spinning Conditions.
http://www.bionmr.com//www.ncbi.nlm.nih.gov/corehtml/query/egifs/http:--www.nature.com-images-lo_npg.gif http://www.bionmr.com//www.ncbi.nlm.nih.gov/corehtml/query/egifs/http:--www.ncbi.nlm.nih.gov-corehtml-pmc-pmcgifs-pubmed-pmc.gif Related Articles A Novel High-Resolution and Sensitivity-Enhanced Three-Dimensional Solid-State NMR Experiment Under Ultrafast Magic Angle Spinning Conditions.
Sci Rep. 2015;5:11810
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08-03-2016 04:58 AM
[NMR paper] Studying Dynamics by Magic-Angle Spinning Solid-State NMR Spectroscopy: Principles and Applications to Biomolecules.
Studying Dynamics by Magic-Angle Spinning Solid-State NMR Spectroscopy: Principles and Applications to Biomolecules.
Studying Dynamics by Magic-Angle Spinning Solid-State NMR Spectroscopy: Principles and Applications to Biomolecules.
Prog Nucl Magn Reson Spectrosc. 2016 Aug;96:1-46
Authors: Schanda P, Ernst M
Abstract
Magic-angle spinning solid-state NMR spectroscopy is an important technique to study molecular structure, dynamics and interactions, and is rapidly gaining importance in biomolecular sciences. Here we provide an...
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04-26-2016 12:14 PM
Studying dynamics by magic-angle spinning solid-state NMR spectroscopy: Principles and applications to biomolecules
Studying dynamics by magic-angle spinning solid-state NMR spectroscopy: Principles and applications to biomolecules
Publication date: August 2016
Source:Progress in Nuclear Magnetic Resonance Spectroscopy, Volume 96</br>
Author(s): Paul Schanda, Matthias Ernst</br>
Magic-angle spinning solid-state NMR spectroscopy is an important technique to study molecular structure, dynamics and interactions, and is rapidly gaining importance in biomolecular sciences. Here we provide an overview of experimental approaches to study molecular dynamics by MAS solid-state NMR,...
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04-09-2016 03:54 AM
Recovery of bulk proton magnetization and sensitivity enhancement in ultrafast magic-angle spinning solid-state NMR
From The DNP-NMR Blog:
Recovery of bulk proton magnetization and sensitivity enhancement in ultrafast magic-angle spinning solid-state NMR
A large portion of the magnetization in a CP experiment remains unused after an experiment and different strategies exist to make better use of the proton magnetization. Here the authors show their results of testing 7 different cp schemes. Although not directly related to DNP these techniques are still very valuable to increase the sensitivity of an NMR experiment especially in combination with DNP.
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03-30-2015 06:04 PM
Recent advances in magic angle spinning solid state NMR of membrane proteins
Recent advances in magic angle spinning solid state NMR of membrane proteins
Publication date: Available online 26 July 2014
Source:Progress in Nuclear Magnetic Resonance Spectroscopy</br>
Author(s): Shenlin Wang , Vladimir Ladizhansky</br>
Membrane proteins mediate many critical functions in cells. Determining their three-dimensional structures in the native lipid environment has been one of the main objectives in structural biology. There are two major NMR methodologies that allow this objective to be accomplished. Oriented sample NMR, which can be applied to...
Ultrafast Magic Angle Spinning Solid-State NMR Spectroscopy: Advances in Methodology and Applications
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