Related ArticlesTwo-dimensional, rotational-echo double-resonance NMR of cell culture metabolism.
J Biol Chem. 1993 Oct 5;268(28):20768-71
Authors: McDowell LM, Cohen ER, Schaefer J
Two-dimensional, rotational-echo double-resonance 13C NMR, a new solid-state NMR technique, has been used to show that the relative fluxes of the labeled chemical bond of L-[2-13C,15N]serine along four metabolic pathways (direct purine synthesis, direct glycine incorporation into protein, direct non-glycyl incorporation into protein, and nitrogen scrambling with loss of carbon) are 1:2:6:36, respectively, for Klebsiella pneumoniae under conditions of nitrogenase derepression. These determinations were performed on a single sample of lyophilized, double-labeled, intact cells. Analysis of the homogeneity of the distribution of label suggests that the primary role of serine in shortening derepression is in providing specific carbon and nitrogen for RNA synthesis.
[NMR paper] Rotational-echo double-resonance NMR-restrained model of the ternary complex of 5-eno
Rotational-echo double-resonance NMR-restrained model of the ternary complex of 5-enolpyruvylshikimate-3-phosphate synthase.
Related Articles Rotational-echo double-resonance NMR-restrained model of the ternary complex of 5-enolpyruvylshikimate-3-phosphate synthase.
J Biomol NMR. 2004 Jan;28(1):11-29
Authors: McDowell LM, Poliks B, Studelska DR, O'Connor RD, Beusen DD, Schaefer J
The 46-kD enzyme 5-enolpyruvylshikimate-3-phosphate (EPSP) synthase catalyzes the condensation of shikimate-3-phosphate (S3P) and phosphoenolpyruvate to form EPSP....
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11-24-2010 09:25 PM
[BMNRC community] In-cell NMR: three-dimensional protein structures
In-cell NMR: three-dimensional protein structures
http://www.nature.com/nature/journal/v458/n7234/edsumm/e090305-13.html
Go to BMNRC community to find more info about this topic.
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08-27-2010 07:58 PM
[NMR paper] Closed form of liganded glutamine-binding protein by rotational-echo double-resonance
Closed form of liganded glutamine-binding protein by rotational-echo double-resonance NMR.
http://www.ncbi.nlm.nih.gov/corehtml/query/egifs/http:--pubs.acs.org-images-acspubs.jpg Related Articles Closed form of liganded glutamine-binding protein by rotational-echo double-resonance NMR.
Biochemistry. 1997 Aug 5;36(31):9405-8
Authors: Klug CA, Tasaki K, Tjandra N, Ho C, Schaefer J
Rotational-echo double-resonance NMR has been used to determine internuclear distances in the complex of glutamine-binding protein and its ligand, l-glutamine. The...
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08-22-2010 05:08 PM
[NMR paper] In situ analysis of peptidyl DOPA in mussel byssus using rotational-echo double-reson
In situ analysis of peptidyl DOPA in mussel byssus using rotational-echo double-resonance NMR.
http://www.ncbi.nlm.nih.gov/corehtml/query/egifs/http:--linkinghub.elsevier.com-ihub-images-PubMedLink.gif Related Articles In situ analysis of peptidyl DOPA in mussel byssus using rotational-echo double-resonance NMR.
Arch Biochem Biophys. 1996 Sep 1;333(1):221-4
Authors: Klug CA, Burzio LA, Waite JH, Schaefer J
Rotational-echo double-resonance (REDOR) 13C NMR spectra with 2H dephasing have been obtained from plaques and threads from the byssus of...
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08-22-2010 02:20 PM
[NMRpipe Yahoo group] Re: problem with fast 3D in echo anti echo mode on Bruker
Re: problem with fast 3D in echo anti echo mode on Bruker
The NMRPipe gradient-shuffling scheme will often result in a spectrum which requires a 90-degree phase correction in the gradient enhanced dimension. In this
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08-18-2010 11:15 AM
[NMRpipe Yahoo group] Re: problem with fast 3D in echo anti echo mode on Bruker
Re: problem with fast 3D in echo anti echo mode on Bruker
Helene Check your second 13C-1H plane; bet all the signal is there. Paul -- Paul R. Gooley, PhD Department of Biochemistry and Molecular Biology and Bio21
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[NMRpipe Yahoo group] problem with fast 3D in echo anti echo mode on Bruker
problem with fast 3D in echo anti echo mode on Bruker
Hi I am trying to process a HNCA 3D experiments acquired with the standard bruker pulse schemes of fast experiments (Brutscher et al) which are recorded with
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