Related ArticlesA Two-Armed Lanthanoid-Chelating Paramagnetic NMR Probe Linked to Proteins via Thioether Linkages.
Chemistry. 2014 Apr 15;
Authors: Liu WM, Skinner SP, Timmer M, Blok A, Hass MA, Filippov DV, Overhand M, Ubbink M
Abstract
Paramagnetic NMR probes provide valuable long-range structural information on proteins and protein complexes. A new, stable, two-armed lanthanoid probe is reported that can be attached to a protein site-specifically via chemically inert thioether linkages.
PMID: 24737492 [PubMed - as supplied by publisher]
The application of paramagnetic lanthanoid ions in NMR spectroscopy on proteins
The application of paramagnetic lanthanoid ions in NMR spectroscopy on proteins
Publication date: Available online 4 November 2013
Source:Coordination Chemistry Reviews</br>
Author(s): Wei-Min Liu , Mark Overhand , Marcellus Ubbink</br>
Lanthanoids are gaining popularity as paramagnetic centers for high resolution nuclear magnetic resonance (NMR) spectroscopy. They provide valuable angular and long-distance restraints for structure calculations of proteins and protein complexes. The introduction of lanthanoids into a protein sample is complicated by the many...
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11-05-2013 06:34 AM
A pH-Sensitive, Colorful,Lanthanide-Chelating ParamagneticNMR Probe
A pH-Sensitive, Colorful,Lanthanide-Chelating ParamagneticNMR Probe
Wei-Min Liu, Peter H. J. Keizers, Mathias A. S. Hass, Anneloes Blok, Monika Timmer, Alexi J. C. Sarris, Mark Overhand and Marcellus Ubbink
http://pubs.acs.org/appl/literatum/publisher/achs/journals/content/jacsat/0/jacsat.ahead-of-print/ja307824e/aop/images/medium/ja-2012-07824e_0011.gif
Journal of the American Chemical Society
DOI: 10.1021/ja307824e
http://feeds.feedburner.com/~ff/acs/jacsat?d=yIl2AUoC8zA
http://feeds.feedburner.com/~r/acs/jacsat/~4/NuKda-fEQQ4
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10-10-2012 03:14 PM
An NMR strategy for fragment-based ligand screening utilizing a paramagnetic lanthanide probe.
An NMR strategy for fragment-based ligand screening utilizing a paramagnetic lanthanide probe.
An NMR strategy for fragment-based ligand screening utilizing a paramagnetic lanthanide probe.
J Biomol NMR. 2011 Sep 17;
Authors: Saio T, Ogura K, Shimizu K, Yokochi M, Burke TR, Inagaki F
Abstract
A nuclear magnetic resonance-based ligand screening strategy utilizing a paramagnetic lanthanide probe is presented. By fixing a paramagnetic lanthanide ion to a target protein, a pseudo-contact shift (PCS) and a paramagnetic relaxation...
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09-20-2011 03:10 PM
An NMR strategy for fragment-based ligand screening utilizing a paramagnetic lanthanide probe
An NMR strategy for fragment-based ligand screening utilizing a paramagnetic lanthanide probe
Abstract A nuclear magnetic resonance-based ligand screening strategy utilizing a paramagnetic lanthanide probe is presented. By fixing a paramagnetic lanthanide ion to a target protein, a pseudo-contact shift (PCS) and a paramagnetic relaxation enhancement (PRE) can be observed for both the target protein and its bound ligand. Based on PRE and PCS information, the bound ligand is then screened from the compound library and the structure of the ligandâ??protein complex is determined. PRE is an...
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09-20-2011 05:02 AM
The use of 1Hâ??31P GHMBC and covariance NMR to unambiguously determine phosphate ester linkages in complex polysaccharide mixtures
The use of 1Hâ??31P GHMBC and covariance NMR to unambiguously determine phosphate ester linkages in complex polysaccharide mixtures
Abstract Poly- and oligo-saccharides are commonly employed as antigens in many vaccines. These antigens contain phosphoester structural elements that are crucial to the antigenicity, and hence the effectiveness of the vaccine. Nuclear Magnetic Resonance (NMR) is a powerful tool for the site-specific identification of phosphoesters in saccharides. We describe here two advances in the characterization of phosphoesters in saccharides: (1) the use of 1Hâ??31P...
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09-19-2011 04:45 AM
[Question from NMRWiki Q&A forum] Tuning probe failed after a dual probe was replaced with a BBI probe
Tuning probe failed after a dual probe was replaced with a BBI probe
We generally use Dual to run 13C and BBI to run 2D. After changed the probe, the command "edhead" was used to set the probe. Put the sample tube, lock the solvent, and then type "atma" to tune the probe. We always do it like this, but now we can not tune the proton after installed the BBI probe (13C is OK). The dip can not be found by "atma", and "atmm" was also not work on forming a dip. What is the most possible reason for this error? How to solve it and avoid it in the future ? Thanks. (Instrument: Bruker 400 MHz,...
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08-23-2011 05:31 PM
[NMR paper] Oxygen as a paramagnetic probe of membrane protein structure by cysteine mutagenesis
Oxygen as a paramagnetic probe of membrane protein structure by cysteine mutagenesis and (19)F NMR spectroscopy.
Related Articles Oxygen as a paramagnetic probe of membrane protein structure by cysteine mutagenesis and (19)F NMR spectroscopy.
J Am Chem Soc. 2002 Feb 27;124(8):1778-81
Authors: Luchette PA, Prosser RS, Sanders CR
Oxygen solubility increases toward the hydrophobic interior of membranes. Using NMR, this O(2) solubility gradient gives rise to an exquisite range of position-dependent paramagnetic effects at partial pressures of 100...
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11-24-2010 08:49 PM
[NMR paper] Probing the surface of a sweet protein: NMR study of MNEI with a paramagnetic probe.
Probing the surface of a sweet protein: NMR study of MNEI with a paramagnetic probe.
Related Articles Probing the surface of a sweet protein: NMR study of MNEI with a paramagnetic probe.
Protein Sci. 2001 Aug;10(8):1498-507
Authors: Niccolai N, Spadaccini R, Scarselli M, Bernini A, Crescenzi O, Spiga O, Ciutti A, Di Maro D, Bracci L, Dalvit C, Temussi PA
The design of safe sweeteners is very important for people who are affected by diabetes, hyperlipemia, and caries and other diseases that are linked to the consumption of sugars. Sweet...
A Two-Armed Lanthanoid-Chelating Paramagnetic NMR Probe Linked to Proteins via Thioether Linkages.
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