[NMR paper] Tumor suppressor p53TAD1-60 forms a fuzzy complex with metastasis associated S100A4: structural insights and dynamics by an NMR/MD approach.
Related ArticlesTumor suppressor p53TAD1-60 forms a fuzzy complex with metastasis associated S100A4: structural insights and dynamics by an NMR/MD approach.
Abstract
Conformationally flexible protein complexes represent a major challenge for structural and dynamical studies. We present a method based on a hybrid NMR/MD approach to characterize the complex formed between the disordered p53TAD 1-60 and the metastasis associated S100A4. Disorder-to-order transition of both TAD1 and TAD2 subdomains upon interaction is detected. Still, p53TAD 1-60 remains highly flexible in the bound form, with residues L26, M40, W53 being anchored to identical hydrophobic pockets of the S100A4 monomer chains. In the resulting "fuzzy" complex the clamp-like binding of p53TAD 1-60 relies on specific hydrophobic anchors and on the existence of extended flexible segments. Our results demonstrate that structural and dynamical NMR parameters (cumulative ??, SSP, temperature coefficients, relaxation time, hetNOE) combined with MD simulations can be used to build a structural model even if due to high flexibility the classical solution structure calculation is not possible.
PMID: 32511842 [PubMed - as supplied by publisher]
[NMR paper] New Structural Insights into Formation of the Key Actin Regulating WIP-WASp Complex Determined by NMR and Molecular Imaging.
New Structural Insights into Formation of the Key Actin Regulating WIP-WASp Complex Determined by NMR and Molecular Imaging.
New Structural Insights into Formation of the Key Actin Regulating WIP-WASp Complex Determined by NMR and Molecular Imaging.
ACS Chem Biol. 2017 Dec 07;:
Authors: Halle-Bikovski A, Fried S, Rozentur-Shkop E, Biber G, Shaked H, Joseph N, Barda-Saad M, Chill JH
Abstract
Wiskott-Aldrich syndrome protein (WASp) is exclusively expressed in hematopoietic cells and responsible for actin-dependent processes,...
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12-08-2017 02:25 PM
Negative regulation of RNA-binding protein HuR by tumor-suppressor ECRG2 - Nature.com
Negative regulation of RNA-binding protein HuR by tumor-suppressor ECRG2 - Nature.com
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Negative regulation of RNA-binding protein HuR by tumor-suppressor ECRG2
Nature.com
ECRG2-mediated growth suppression was associated with activation of caspases and marked reduction in the levels of apoptosis inhibitor, X chromosome-linked inhibitor of apoptosis protein (XIAP). ECRG2, via RNA-binding protein human antigen R (HuR ...
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Biochemists reveal interaction between tumor suppressor protein and chaperone - News-Medical.net
Biochemists reveal interaction between tumor suppressor protein and chaperone - News-Medical.net
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Biochemists reveal interaction between tumor suppressor protein and chaperone
News-Medical.net
Using nuclear magnetic resonance (NMR) spectroscopy, the scientists at the Bavarian NMR Center in Garching were able for the first time to characterize the interaction surfaces between Hsp90 and p53 and show that p53 binds to Hsp90 in an already ...
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09-08-2011 08:24 AM
Investigation of solvent effect and NMR shielding tensors of p53 tumor-suppressor gene in drug design.
Investigation of solvent effect and NMR shielding tensors of p53 tumor-suppressor gene in drug design.
http://www.ncbi.nlm.nih.gov/corehtml/query/egifs/http:--www.pubmedcentral.nih.gov-corehtml-pmc-pmcgifs-pubmed-pmc.gif Investigation of solvent effect and NMR shielding tensors of p53 tumor-suppressor gene in drug design.
Int J Nanomedicine. 2011;6:213-8
Authors: Irani S, Monajjemi M, Honarparvar B, Atyabi S, Sadeghizadeh M
Abstract
The p53 tumor-suppressor gene encodes a nuclear phosphoprotein with cancer- inhibiting properties. The...
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08-25-2011 04:10 PM
[NMR paper] Tumor suppressor INK4: refinement of p16INK4A structure and determination of p15INK4B
Tumor suppressor INK4: refinement of p16INK4A structure and determination of p15INK4B structure by comparative modeling and NMR data.
Related Articles Tumor suppressor INK4: refinement of p16INK4A structure and determination of p15INK4B structure by comparative modeling and NMR data.
Protein Sci. 2000 Jun;9(6):1120-8
Authors: Yuan C, Selby TL, Li J, Byeon IJ, Tsai MD
Within the tumor suppressor protein INK4 (inhibitor of cyclin-dependent kinase 4) family, p15INK4B is the smallest and the only one whose structure has not been determined...
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11-18-2010 09:15 PM
[NMR paper] The Aspergillus nidulans transcription factor AlcR forms a stable complex with its ha
The Aspergillus nidulans transcription factor AlcR forms a stable complex with its half-site DNA: a NMR study.
http://www.ncbi.nlm.nih.gov/corehtml/query/egifs/http:--linkinghub.elsevier.com-ihub-images-PubMedLink.gif Related Articles The Aspergillus nidulans transcription factor AlcR forms a stable complex with its half-site DNA: a NMR study.
FEBS Lett. 1997 May 19;408(2):235-40
Authors: Cerdan R, Collin D, Lenouvel F, Felenbok B, Guittet E
The Aspergillus nidulans transcription factor AlcR is shown by NMR and gel retardation assay to form a...
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[NMR paper] Tumor suppressor p16INK4A: structural characterization of wild-type and mutant protei
Tumor suppressor p16INK4A: structural characterization of wild-type and mutant proteins by NMR and circular dichroism.
http://www.ncbi.nlm.nih.gov/corehtml/query/egifs/http:--pubs.acs.org-images-acspubs.jpg Related Articles Tumor suppressor p16INK4A: structural characterization of wild-type and mutant proteins by NMR and circular dichroism.
Biochemistry. 1996 Jul 23;35(29):9475-87
Authors: Tevelev A, Byeon IJ, Selby T, Ericson K, Kim HJ, Kraynov V, Tsai MD
The tumor suppressor p16INK4A with eight N-terminal amino acids deleted (p16/delta 1-8)...
Tumor suppressor p53TAD1-60 forms a fuzzy complex with metastasis associated S100A4: structural insights and dynamics by an NMR/MD approach.
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