Related ArticlesTrimethylsilyl reporter groups for NMR studies of conformational changes in G protein-coupled receptors.
FEBS Lett. 2019 Apr 05;:
Authors: Hu W, Wang H, Hou Y, Hao Y, Liu D
Abstract
Large membrane proteins such as G Protein-Coupled Receptors (GPCRs) are difficult for NMR study due to severe signal overlaps and unfavorable relaxation properties. We used a trimethylsilyl (TMS) group as a reporter group for 1 H NMR study of conformational changes in proteins, utilizing high-intensity 1 H NMR signals near 0 ppm. The ?2 -Adrenergic Receptor (?2 AR) was labeled with TMS groups at two cysteines located at the cytoplasmic ends of helices VI and VII. Binding of various ligands led to changes in 1 H NMR signals, which manifested that helix VI is sensitive to G protein-specific activation, whereas helix VII is sensitive to ?-arrestin-specific activation. Thus, the TMS group is a useful reporter group in NMR for studying conformational changes in membrane proteins such as GPCRs. This article is protected by copyright. All rights reserved.
PMID: 30953343 [PubMed - as supplied by publisher]
[NMR paper] Universality of Sodium Ion Binding Mechanism in Class A G-Protein-Coupled Receptors
Universality of Sodium Ion Binding Mechanism in Class A G-Protein-Coupled Receptors
The allosteric modulation of GPCRs by sodium (Na+) ions has drawn significant attention due to the availability of crystal structures of several receptors showing Na+ ions bound to the inactive conformations at the conserved Asp2.50. To date, structures from 24 families of GPCRs have been determined, though mechanistic insights into Na+ binding to the allosteric site are limited. We performed 100's of ?s long simulations of 15 GPCRs and elucidated their Na+ binding mechanism. In class A GPCRs, Na+ ion...
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[NMR paper] Genetically encoded amino acids with tert-butyl and trimethylsilyl groups for site-selective studies of proteins by NMR spectroscopy.
Genetically encoded amino acids with tert-butyl and trimethylsilyl groups for site-selective studies of proteins by NMR spectroscopy.
Genetically encoded amino acids with tert-butyl and trimethylsilyl groups for site-selective studies of proteins by NMR spectroscopy.
J Biomol NMR. 2017 Dec 02;:
Authors: Loh CT, Adams LA, Graham B, Otting G
Abstract
The amino acids 4-(tert-butyl)phenylalanine (Tbf) and 4-(trimethylsilyl)phenylalanine (TMSf), as well as a partially deuterated version of Tbf (dTbf), were chemically synthesized and...
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Genetically encoded amino acids with tert -butyl and trimethylsilyl groups for site-selective studies of proteins by NMR spectroscopy
Genetically encoded amino acids with tert -butyl and trimethylsilyl groups for site-selective studies of proteins by NMR spectroscopy
Abstract
The amino acids 4-(tert-butyl)phenylalanine (Tbf) and 4-(trimethylsilyl)phenylalanine (TMSf), as well as a partially deuterated version of Tbf (dTbf), were chemically synthesized and site-specifically incorporated into different proteins, using an amber stop codon, suppressor tRNA and the broadband aminoacyl-tRNA synthetase originally evolved for the incorporation of p-cyano-phenylalanine. The 1H-NMR signals of...
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Global G-Protein Coupled Receptors (GPCRs) Industry - Sacramento Bee
Global G-Protein Coupled Receptors (GPCRs) Industry - Sacramento Bee
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Global G-Protein Coupled Receptors (GPCRs) Industry
Sacramento Bee
NMR Spectroscopy: An Invaluable Tool to Study GPCR Structures II-4 ... G-PROTEIN COUPLED RECEPTORS (GPCR) - A CONCEPTUAL OVERVIEW II-7GPCR: Gateways to Cell II-7Classification of GPCRs II-7Table 1: List of Identified Known and Orphan (Non-Sensory)GPCRs ...
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08-15-2012 08:54 AM
Global G-Protein Coupled Receptors (GPCRs) Industry - Sacramento Bee
Global G-Protein Coupled Receptors (GPCRs) Industry - Sacramento Bee
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Global G-Protein Coupled Receptors (GPCRs) Industry
Sacramento Bee
This report analyzes the Global market for G-Protein Coupled Receptors (GPCRs) in ... NMR Spectroscopy: An Invaluable Tool to Study GPCR Structures II-4 ...
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06-13-2012 11:34 AM
Structure and function of G protein-coupled receptors using NMR spectroscopy
Structure and function of G protein-coupled receptors using NMR spectroscopy
Publication year: 2010
Source:Progress in Nuclear Magnetic Resonance Spectroscopy, Volume 57, Issue 2</br>
Joseph A. Goncalves, Shivani Ahuja, Sina Erfani, Markus Eilers, Steven O. Smith</br>
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03-09-2012 09:16 AM
[NMR paper] Peptide hormone binding to G-protein-coupled receptors: structural characterization v
Peptide hormone binding to G-protein-coupled receptors: structural characterization via NMR techniques.
Related Articles Peptide hormone binding to G-protein-coupled receptors: structural characterization via NMR techniques.
Med Res Rev. 2001 Sep;21(5):450-71
Authors: Mierke DF, Giragossian C
G-protein-coupled receptors (GPCRs) allow cells to respond to calcium, hormones, and neurotransmitters. Not surprisingly, they currently make up the largest family of validated drug targets. Rational drug design for molecular regulators targeting GPCRs...
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11-19-2010 08:44 PM
Structure and function of G protein-coupled receptors using NMR spectroscopy
Structure and function of G protein-coupled receptors using NMR spectroscopy
Publication year: 2010
Source: Progress in Nuclear Magnetic Resonance Spectroscopy, In Press, Accepted Manuscript, Available online 12 May 2010</br>
Joseph A., Goncalves , Shivani, Ahuja , Sina, Erfani , Markus, Eilers , Steven O., Smith</br>
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Trimethylsilyl reporter groups for NMR studies of conformational changes in G protein-coupled receptors.
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