Related ArticlesTransverse dephasing optimized solid-state NMR spectroscopy.
J Am Chem Soc. 2003 Nov 19;125(46):13938-9
Authors: De Paëpe G, Giraud N, Lesage A, Hodgkinson P, Böckmann A, Emsley L
It is shown how coherence lifetimes in solid-state NMR experiments can be controlled. New decoupling schemes are introduced which actively optimize dephasing times, providing increases of up to a factor of 2 with respect to the best existing schemes. The new schemes are implemented in transverse-dephasing-optimized (TDOP) NMR experiments for the disorded solid cellulose, and for a microcrystalline protein, where sensitivity improvements of up to a factor of 5 are obtained.
Translational Diffusion of Macromolecular Assemblies Measured Using Transverse-Relaxation-Optimized Pulsed Field Gradient NMR
Translational Diffusion of Macromolecular Assemblies Measured Using Transverse-Relaxation-Optimized Pulsed Field Gradient NMR
Reto Horst, Arthur L. Horwich and Kurt Wu?thrich
http://pubs.acs.org/appl/literatum/publisher/achs/journals/content/jacsat/0/jacsat.ahead-of-print/ja206531c/aop/images/medium/ja-2011-06531c_0003.gif
Journal of the American Chemical Society
DOI: 10.1021/ja206531c
http://feeds.feedburner.com/~ff/acs/jacsat?d=yIl2AUoC8zA
http://feeds.feedburner.com/~r/acs/jacsat/~4/NWK45WCbths
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09-26-2011 06:54 PM
Translational diffusion of macromolecular assemblies measured using transverse relaxation-optimized PFG-NMR.
Translational diffusion of macromolecular assemblies measured using transverse relaxation-optimized PFG-NMR.
Translational diffusion of macromolecular assemblies measured using transverse relaxation-optimized PFG-NMR.
J Am Chem Soc. 2011 Sep 16;
Authors: Horst R, Horwich AL, Wüthrich K
Abstract
ABSTRACT In structural biology, pulsed field gradient (PFG) NMR for characterization of size and hydrodynamic parameters of macromolecular solutes has the advantage over other techniques that the measurements can be recorded with identical solution...
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09-17-2011 08:21 PM
Solid-state NMR spectroscopy on complex biomolecules.
Solid-state NMR spectroscopy on complex biomolecules.
Solid-state NMR spectroscopy on complex biomolecules.
Angew Chem Int Ed Engl. 2010 Nov 2;49(45):8346-57
Authors: Renault M, Cukkemane A, Baldus M
Biomolecular applications of NMR spectroscopy are often merely associated with soluble molecules or magnetic resonance imaging. However, since the late 1970s, solid-state NMR (ssNMR) spectroscopy has demonstrated its ability to provide atomic-level insight into complex biomolecular systems ranging from lipid bilayers to complex biomaterials. In the...
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02-15-2011 07:17 PM
[NMR paper] Relaxation-optimized NMR spectroscopy of methylene groups in proteins and nucleic aci
Relaxation-optimized NMR spectroscopy of methylene groups in proteins and nucleic acids.
Related Articles Relaxation-optimized NMR spectroscopy of methylene groups in proteins and nucleic acids.
J Am Chem Soc. 2004 Sep 1;126(34):10560-70
Authors: Miclet E, Williams Jr DC, Clore GM, Bryce DL, Boisbouvier J, Bax A
A large fraction of hydrogens in proteins and nucleic acids is of the methylene type. Their detailed study, however, in terms of structure and dynamics by NMR spectroscopy is hampered by their fast relaxation properties, which give...
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11-24-2010 10:01 PM
[NMR paper] Sensitivity enhancement in two-dimensional solid-state NMR spectroscopy by transverse
Sensitivity enhancement in two-dimensional solid-state NMR spectroscopy by transverse mixing.
Related Articles Sensitivity enhancement in two-dimensional solid-state NMR spectroscopy by transverse mixing.
Chemphyschem. 2004 Jun 21;5(6):863-8
Authors: Tycko R
The sensitivity of two-dimensional (2D) 13C-13C solid-state NMR spectroscopy under magic-angle spinning (MAS) is shown to be enhanced by the use of transverse polarization transfer in place of the conventional longitudinal polarization transfer. Experimental results are reported for 2D...
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11-24-2010 09:51 PM
[NMR paper] Hydration-optimized oriented phospholipid bilayer samples for solid-state NMR structu
Hydration-optimized oriented phospholipid bilayer samples for solid-state NMR structural studies of membrane proteins.
Related Articles Hydration-optimized oriented phospholipid bilayer samples for solid-state NMR structural studies of membrane proteins.
J Magn Reson. 2003 Mar;161(1):64-9
Authors: Marassi FM, Crowell KJ
The preparation of oriented, hydration-optimized lipid bilayer samples, for NMR structure determination of membrane proteins, is described. The samples consist of planar phospholipid bilayers, containing membrane proteins, that...
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11-24-2010 09:01 PM
[NMR paper] Transverse relaxation-optimized NMR spectroscopy with the outer membrane protein OmpX
Transverse relaxation-optimized NMR spectroscopy with the outer membrane protein OmpX in dihexanoyl phosphatidylcholine micelles.
Related Articles Transverse relaxation-optimized NMR spectroscopy with the outer membrane protein OmpX in dihexanoyl phosphatidylcholine micelles.
Proc Natl Acad Sci U S A. 2001 Feb 27;98(5):2358-63
Authors: Fernández C, Adeishvili K, Wüthrich K
The (2)H,(13)C,(15)N-labeled, 148-residue integral membrane protein OmpX from Escherichia coli was reconstituted with dihexanoyl phosphatidylcholine (DHPC) in mixed micelles...
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11-19-2010 08:32 PM
GFT projection NMR spectroscopy for proteins in the solid state.
GFT projection NMR spectroscopy for proteins in the solid state.
GFT projection NMR spectroscopy for proteins in the solid state.
J Biomol NMR. 2010 Oct 30;
Authors: Trent Franks W, Atreya HS, Szyperski T, Rienstra CM
Recording of four-dimensional (4D) spectra for proteins in the solid state has opened new avenues to obtain virtually complete resonance assignments and three-dimensional (3D) structures of proteins. As in solution state NMR, the sampling of three indirect dimensions leads per se to long minimal measurement time. Furthermore,...
Transverse dephasing optimized solid-state NMR spectroscopy.
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