[NMR paper] Translational Diffusion and Self-Association of an Intrinsically Disordered Protein kappa-Casein Using NMR with Ultra-High Pulsed-Field Gradient and Time-Resolved FRET
Translational Diffusion and Self-Association of an Intrinsically Disordered Protein kappa-Casein Using NMR with Ultra-High Pulsed-Field Gradient and Time-Resolved FRET
Much attention has been given to studying the translational diffusion of globular proteins, whereas the translational diffusion of intrinsically disordered proteins (IDPs) is less studied. In this study, we investigate the translational diffusion and how it is affected by the self-association of an IDP, ?-casein, using pulsed-field gradient nuclear magnetic resonance and time-resolved Förster resonance energy transfer. Using the analysis of the shape of diffusion attenuation and the...
[NMR paper] Surface Accessibility of an Intrinsically Disordered Protein Probed by 2D Time-Resolved Laser-Assisted NMR Spectroscopy
Surface Accessibility of an Intrinsically Disordered Protein Probed by 2D Time-Resolved Laser-Assisted NMR Spectroscopy
Probing the protein surface accessibility of different residues is a powerful way of characterizing the overall conformation of intrinsically disordered proteins (IDPs). We present a two-dimensional (2D) time-resolved photo-CIDNP (TR-CIDNP) experiment suitable for IDP analysis. Pulse stretching of high-power laser pulses, band-selective decoupling of ^(13)C^(?), and simultaneous application of radiofrequency and laser pulses were implemented to quantitatively analyze the...
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[NMR paper] Obtaining Hydrodynamic Radii of Intrinsically Disordered Protein Ensembles by Pulsed Field Gradient NMR Measurements.
Obtaining Hydrodynamic Radii of Intrinsically Disordered Protein Ensembles by Pulsed Field Gradient NMR Measurements.
Related Articles Obtaining Hydrodynamic Radii of Intrinsically Disordered Protein Ensembles by Pulsed Field Gradient NMR Measurements.
Methods Mol Biol. 2020;2141:285-302
Authors: Leeb S, Danielsson J
Abstract
In the disordered state, a protein exhibits a high degree of structural freedom, in both space and time. For an ensemble of disordered or unfolded proteins, this means that the ensemble comprises a high...
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[NMR paper] Protein structural changes characterized by high-pressure, pulsed field gradient diffusion NMR spectroscopy.
Protein structural changes characterized by high-pressure, pulsed field gradient diffusion NMR spectroscopy.
http://www.bionmr.com//www.ncbi.nlm.nih.gov/corehtml/query/egifs/https:--linkinghub.elsevier.com-ihub-images-PubMedLink.gif Related Articles Protein structural changes characterized by high-pressure, pulsed field gradient diffusion NMR spectroscopy.
J Magn Reson. 2020 Feb 19;312:106701
Authors: Ramanujam V, Alderson TR, Pritišanac I, Ying J, Bax A
Abstract
Pulsed-field gradient NMR spectroscopy is widely used to measure...
Translational Diffusion of Macromolecular Assemblies Measured Using Transverse-Relaxation-Optimized Pulsed Field Gradient NMR
Translational Diffusion of Macromolecular Assemblies Measured Using Transverse-Relaxation-Optimized Pulsed Field Gradient NMR
Reto Horst, Arthur L. Horwich and Kurt Wu?thrich
http://pubs.acs.org/appl/literatum/publisher/achs/journals/content/jacsat/0/jacsat.ahead-of-print/ja206531c/aop/images/medium/ja-2011-06531c_0003.gif
Journal of the American Chemical Society
DOI: 10.1021/ja206531c
http://feeds.feedburner.com/~ff/acs/jacsat?d=yIl2AUoC8zA
http://feeds.feedburner.com/~r/acs/jacsat/~4/NWK45WCbths
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[NMR paper] Diffusion of polyethyleneglycols in casein solutions and gels as studied by pulsed fi
Diffusion of polyethyleneglycols in casein solutions and gels as studied by pulsed field gradient NMR.
Related Articles Diffusion of polyethyleneglycols in casein solutions and gels as studied by pulsed field gradient NMR.
Magn Reson Imaging. 2005 Feb;23(2):347-8
Authors: Colsenet R, Soderman O, Mariette F
Molecular transport characterized by diffusion coefficients is a key feature of food processes and especially in dairy processes. Caseins represent 80% of the protein content in milk and are directly involved in the formation of dairy gels....
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11-24-2010 11:14 PM
[NMR paper] Slow diffusion of macromolecular assemblies by a new pulsed field gradient NMR method
Slow diffusion of macromolecular assemblies by a new pulsed field gradient NMR method.
Related Articles Slow diffusion of macromolecular assemblies by a new pulsed field gradient NMR method.
J Am Chem Soc. 2003 Mar 5;125(9):2541-5
Authors: Ferrage F, Zoonens M, Warschawski DE, Popot JL, Bodenhausen G
The translational diffusion coefficient of an integral membrane protein/surfactant complex has been measured using a novel pulsed field gradient NMR method. In this new approach, the information about the localization of the molecules is...
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[NMR paper] Measuring protein self-association using pulsed-field-gradient NMR spectroscopy: appl
Measuring protein self-association using pulsed-field-gradient NMR spectroscopy: application to myosin light chain 2.
Related Articles Measuring protein self-association using pulsed-field-gradient NMR spectroscopy: application to myosin light chain 2.
J Biomol NMR. 1995 Nov;6(3):321-8
Authors: Dingley AJ, Mackay JP, Chapman BE, Morris MB, Kuchel PW, Hambly BD, King GF
At the millimolar concentrations required for structural studies, NMR spectra of the calcium-binding protein myosin light chain 2 (MLC2) showed resonance line widths indicative...
Translational Diffusion and Self-Association of an Intrinsically Disordered Protein kappa-Casein Using NMR with Ultra-High Pulsed-Field Gradient and Time-Resolved FRET
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